Reviewed, UniProtKB/Swiss-Prot Q04604 (TYRO_RANNI)

Names and origin

Protein names

Recommended name:
    Tyrosinase
    EC=1.14.18.1
Alternative name(s):
    Monophenol monooxygenase

Gene names

Name:	TYR
Synonyms:	TYRS

Organism

Rana nigromaculata (Japanese pond frog)

Taxonomic identifier

8409 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Neobatrachia › Ranoidea › Ranidae › Raninae › Rana › Pelophylax

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.
Catalytic activity	L-tyrosine + L-dopa + O2 = L-dopa + dopaquinone + H2O.
Cofactor	Binds 2 copper ions per subunit.
Subcellular location	Melanosome membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the tyrosinase family.

Ontologies

Keywords
Biological process	Melanin biosynthesis
Cellular component	Membrane
Domain	Signal Transmembrane
Ligand	Copper Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	melanin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW monophenol monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 532	513	Tyrosinase		PRO_0000035884
Regions
	Topological domain	20 – 475	456	Lumenal, melanosome Potential
	Transmembrane	476 – 499	24	Potential
	Topological domain	500 – 532	33	Cytoplasmic Potential
Sites
	Metal binding	184	1	Copper A By similarity
	Metal binding	206	1	Copper A By similarity
	Metal binding	215	1	Copper A By similarity
	Metal binding	367	1	Copper B By similarity
	Metal binding	371	1	Copper B By similarity
	Metal binding	394	1	Copper B By similarity
Amino acid modifications
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	165	1	N-linked (GlcNAc...) Potential
	Glycosylation	234	1	N-linked (GlcNAc...) Potential
	Glycosylation	341	1	N-linked (GlcNAc...) Potential
	Glycosylation	375	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	10	1	T → A

Sequences

	Sequence		Length	Mass (Da)
	Q04604-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: B27D3080F0C74B3A	FASTA	532	60,116
	10 20 30 40 50 60 MESTTVLLAT STLLLVLHAS YGQFPRACST AQVLLSKECC PVWPGDNSSC GEVSGRGVCQ 70 80 90 100 110 120 DVVPSNSPVG AQFPFSGIDD RENWPIVFYN RTCQCQGNFM GYNCGECRFG YTGPNCTVRR 130 140 150 160 170 180 NMIRKDIFRM TTAEKDKLIA YLNLAKHTIS PDYVIATGTY EQMNNGSNPM FADISAYDLF 190 200 210 220 230 240 VWIHYYASRD AFLEDGSVWA DIDFAHEAPG FLPWHRFYLL LWEREIQKVT GDDNFTIPFW 250 260 270 280 290 300 DWRDAQQCEL CTDEFFGGTH PTSNNLLSPA SFFSSWQIIC SRPEEYNSLR IICNGTNEGP 310 320 330 340 350 360 LLRSPGRHDR NRTPRLPTSA DVEACLSLTD YETGAMDRSA NFSFRNTLEG FAVPTSGIAN 370 380 390 400 410 420 RSQSSMHNSL HVFLNGSMSS VQGSANDPIF VLHHAFVDSL FEQWLRRHQP SLDVYPEANA 430 440 450 460 470 480 PVGHNREYNM VPFIPLFTNG EFFVQSRDLG YDYDYLAESG SIEDFLLPYL EQARQIWQWL 490 500 510 520 530 LGAAVLGGLI TAVIATIISL TCRRKRKTKI SEETRPLLME AEDYQATYQS NL

References

[1]	"Cloning and sequencing of the cDNA encoding tyrosinase of the Japanese pond frog, Rana nigromaculata." Takase M., Miura I., Nakata A., Takeuchi T., Nishioka M. Gene 121:359-363(1992) [PubMed: 1446833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Analysis of the tyrosinase gene of the Japanese pond frog, Rana nigromaculata: cloning and nucleotide sequence of the genomic DNA containing the tyrosinase gene and its flanking regions." Miura I., Okumoto H., Makino K., Nakata A., Nishioka M. Jpn. J. Genet. 70:79-92(1995) [PubMed: 7772385] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-277. Tissue: Blood.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12514 mRNA. Translation: BAA02077.1. D37779 Genomic DNA. Translation: BAA07034.1.
PIR	JC1392.
3D structure databases
SMR	Q04604. Positions 122-491.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q04604.
Enzyme and pathway databases
BRENDA	1.14.18.1. 2485.
Family and domain databases
InterPro	IPR008922. Di-copper_centre. IPR002227. Tyrosinase. [Graphical view]
Gene3D	G3DSA:1.10.1280.10. Di-copper_centre. 1 hit.
Pfam	PF00264. Tyrosinase. 1 hit. [Graphical view]
PRINTS	PR00092. TYROSINASE.
PROSITE	PS00497. TYROSINASE_1. 1 hit. PS00498. TYROSINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYRO_RANNI

Accession

Primary (citable) accession number: Q04604

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 19, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)