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Protein

Tyrosinase

Gene

TYR

Organism
Pelophylax nigromaculatus (Black-spotted frog) (Rana nigromaculata)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
L-tyrosine + O2 = dopaquinone + H2O.

Cofactori

Cu2+Note: Binds 2 copper ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi184 – 1841Copper ABy similarity
Metal bindingi206 – 2061Copper ABy similarity
Metal bindingi215 – 2151Copper ABy similarity
Metal bindingi367 – 3671Copper BBy similarity
Metal bindingi371 – 3711Copper BBy similarity
Metal bindingi394 – 3941Copper BBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. monophenol monooxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. melanin biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosinase (EC:1.14.18.1)
Alternative name(s):
Monophenol monooxygenase
Gene namesi
Name:TYR
Synonyms:TYRS
OrganismiPelophylax nigromaculatus (Black-spotted frog) (Rana nigromaculata)
Taxonomic identifieri8409 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaRanoideaRanidaePelophylax

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 475456Lumenal, melanosomeSequence AnalysisAdd
BLAST
Transmembranei476 – 49924HelicalSequence AnalysisAdd
BLAST
Topological domaini500 – 53233CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. melanosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 532513TyrosinasePRO_0000035884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi234 – 2341N-linked (GlcNAc...)Sequence Analysis
Glycosylationi341 – 3411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ04604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG003553.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESTTVLLAT STLLLVLHAS YGQFPRACST AQVLLSKECC PVWPGDNSSC
60 70 80 90 100
GEVSGRGVCQ DVVPSNSPVG AQFPFSGIDD RENWPIVFYN RTCQCQGNFM
110 120 130 140 150
GYNCGECRFG YTGPNCTVRR NMIRKDIFRM TTAEKDKLIA YLNLAKHTIS
160 170 180 190 200
PDYVIATGTY EQMNNGSNPM FADISAYDLF VWIHYYASRD AFLEDGSVWA
210 220 230 240 250
DIDFAHEAPG FLPWHRFYLL LWEREIQKVT GDDNFTIPFW DWRDAQQCEL
260 270 280 290 300
CTDEFFGGTH PTSNNLLSPA SFFSSWQIIC SRPEEYNSLR IICNGTNEGP
310 320 330 340 350
LLRSPGRHDR NRTPRLPTSA DVEACLSLTD YETGAMDRSA NFSFRNTLEG
360 370 380 390 400
FAVPTSGIAN RSQSSMHNSL HVFLNGSMSS VQGSANDPIF VLHHAFVDSL
410 420 430 440 450
FEQWLRRHQP SLDVYPEANA PVGHNREYNM VPFIPLFTNG EFFVQSRDLG
460 470 480 490 500
YDYDYLAESG SIEDFLLPYL EQARQIWQWL LGAAVLGGLI TAVIATIISL
510 520 530
TCRRKRKTKI SEETRPLLME AEDYQATYQS NL
Length:532
Mass (Da):60,116
Last modified:September 30, 1993 - v1
Checksum:iB27D3080F0C74B3A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti10 – 101T → A.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12514 mRNA. Translation: BAA02077.1.
D37779 Genomic DNA. Translation: BAA07034.1.
PIRiJC1392.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12514 mRNA. Translation: BAA02077.1.
D37779 Genomic DNA. Translation: BAA07034.1.
PIRiJC1392.

3D structure databases

ProteinModelPortaliQ04604.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG003553.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of the cDNA encoding tyrosinase of the Japanese pond frog, Rana nigromaculata."
    Takase M., Miura I., Nakata A., Takeuchi T., Nishioka M.
    Gene 121:359-363(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Analysis of the tyrosinase gene of the Japanese pond frog, Rana nigromaculata: cloning and nucleotide sequence of the genomic DNA containing the tyrosinase gene and its flanking regions."
    Miura I., Okumoto H., Makino K., Nakata A., Nishioka M.
    Jpn. J. Genet. 70:79-92(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-277.
    Tissue: Blood.

Entry informationi

Entry nameiTYRO_PELNI
AccessioniPrimary (citable) accession number: Q04604
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1993
Last sequence update: September 30, 1993
Last modified: January 6, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.