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Q04604

- TYRO_PELNI

UniProt

Q04604 - TYRO_PELNI

Protein

Tyrosinase

Gene

TYR

Organism
Pelophylax nigromaculatus (Black-spotted frog) (Rana nigromaculata)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.

    Catalytic activityi

    2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
    L-tyrosine + O2 = dopaquinone + H2O.

    Cofactori

    Binds 2 copper ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi184 – 1841Copper ABy similarity
    Metal bindingi206 – 2061Copper ABy similarity
    Metal bindingi215 – 2151Copper ABy similarity
    Metal bindingi367 – 3671Copper BBy similarity
    Metal bindingi371 – 3711Copper BBy similarity
    Metal bindingi394 – 3941Copper BBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. monophenol monooxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. melanin biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Melanin biosynthesis

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosinase (EC:1.14.18.1)
    Alternative name(s):
    Monophenol monooxygenase
    Gene namesi
    Name:TYR
    Synonyms:TYRS
    OrganismiPelophylax nigromaculatus (Black-spotted frog) (Rana nigromaculata)
    Taxonomic identifieri8409 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaRanoideaRanidaePelophylax

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. melanosome membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 532513TyrosinasePRO_0000035884Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi234 – 2341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi341 – 3411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ04604.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 475456Lumenal, melanosomeSequence AnalysisAdd
    BLAST
    Topological domaini500 – 53233CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei476 – 49924HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tyrosinase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG003553.

    Family and domain databases

    Gene3Di1.10.1280.10. 1 hit.
    InterProiIPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view]
    PfamiPF00264. Tyrosinase. 1 hit.
    [Graphical view]
    PRINTSiPR00092. TYROSINASE.
    SUPFAMiSSF48056. SSF48056. 1 hit.
    PROSITEiPS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04604-1 [UniParc]FASTAAdd to Basket

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    MESTTVLLAT STLLLVLHAS YGQFPRACST AQVLLSKECC PVWPGDNSSC    50
    GEVSGRGVCQ DVVPSNSPVG AQFPFSGIDD RENWPIVFYN RTCQCQGNFM 100
    GYNCGECRFG YTGPNCTVRR NMIRKDIFRM TTAEKDKLIA YLNLAKHTIS 150
    PDYVIATGTY EQMNNGSNPM FADISAYDLF VWIHYYASRD AFLEDGSVWA 200
    DIDFAHEAPG FLPWHRFYLL LWEREIQKVT GDDNFTIPFW DWRDAQQCEL 250
    CTDEFFGGTH PTSNNLLSPA SFFSSWQIIC SRPEEYNSLR IICNGTNEGP 300
    LLRSPGRHDR NRTPRLPTSA DVEACLSLTD YETGAMDRSA NFSFRNTLEG 350
    FAVPTSGIAN RSQSSMHNSL HVFLNGSMSS VQGSANDPIF VLHHAFVDSL 400
    FEQWLRRHQP SLDVYPEANA PVGHNREYNM VPFIPLFTNG EFFVQSRDLG 450
    YDYDYLAESG SIEDFLLPYL EQARQIWQWL LGAAVLGGLI TAVIATIISL 500
    TCRRKRKTKI SEETRPLLME AEDYQATYQS NL 532
    Length:532
    Mass (Da):60,116
    Last modified:October 1, 1993 - v1
    Checksum:iB27D3080F0C74B3A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti10 – 101T → A.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D12514 mRNA. Translation: BAA02077.1.
    D37779 Genomic DNA. Translation: BAA07034.1.
    PIRiJC1392.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D12514 mRNA. Translation: BAA02077.1 .
    D37779 Genomic DNA. Translation: BAA07034.1 .
    PIRi JC1392.

    3D structure databases

    ProteinModelPortali Q04604.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG003553.

    Family and domain databases

    Gene3Di 1.10.1280.10. 1 hit.
    InterProi IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view ]
    Pfami PF00264. Tyrosinase. 1 hit.
    [Graphical view ]
    PRINTSi PR00092. TYROSINASE.
    SUPFAMi SSF48056. SSF48056. 1 hit.
    PROSITEi PS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the cDNA encoding tyrosinase of the Japanese pond frog, Rana nigromaculata."
      Takase M., Miura I., Nakata A., Takeuchi T., Nishioka M.
      Gene 121:359-363(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Analysis of the tyrosinase gene of the Japanese pond frog, Rana nigromaculata: cloning and nucleotide sequence of the genomic DNA containing the tyrosinase gene and its flanking regions."
      Miura I., Okumoto H., Makino K., Nakata A., Nishioka M.
      Jpn. J. Genet. 70:79-92(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-277.
      Tissue: Blood.

    Entry informationi

    Entry nameiTYRO_PELNI
    AccessioniPrimary (citable) accession number: Q04604
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3