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Protein

Vacuolar basic amino acid transporter 4

Gene

VBA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transporter required for vacuolar uptake of basic amino acids.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29719-MONOMER.

Protein family/group databases

TCDBi2.A.1.48.5. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar basic amino acid transporter 4
Gene namesi
Name:VBA4
Ordered Locus Names:YDR119W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR119W.
SGDiS000002526. VBA4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 252252CytoplasmicSequence analysisAdd
BLAST
Transmembranei253 – 27321HelicalSequence analysisAdd
BLAST
Topological domaini274 – 2829VacuolarSequence analysis
Transmembranei283 – 30523HelicalSequence analysisAdd
BLAST
Topological domaini306 – 3116CytoplasmicSequence analysis
Transmembranei312 – 33120HelicalSequence analysisAdd
BLAST
Topological domaini332 – 3343VacuolarSequence analysis
Transmembranei335 – 35723HelicalSequence analysisAdd
BLAST
Topological domaini358 – 37518CytoplasmicSequence analysisAdd
BLAST
Transmembranei376 – 39621HelicalSequence analysisAdd
BLAST
Topological domaini397 – 40610VacuolarSequence analysis
Transmembranei407 – 42721HelicalSequence analysisAdd
BLAST
Topological domaini428 – 44720CytoplasmicSequence analysisAdd
BLAST
Transmembranei448 – 46821HelicalSequence analysisAdd
BLAST
Topological domaini469 – 48113VacuolarSequence analysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence analysisAdd
BLAST
Topological domaini503 – 52220CytoplasmicSequence analysisAdd
BLAST
Transmembranei523 – 54321HelicalSequence analysisAdd
BLAST
Topological domaini544 – 56219VacuolarSequence analysisAdd
BLAST
Transmembranei563 – 58321HelicalSequence analysisAdd
BLAST
Topological domaini584 – 5874CytoplasmicSequence analysis
Transmembranei588 – 60821HelicalSequence analysisAdd
BLAST
Topological domaini609 – 6179VacuolarSequence analysis
Transmembranei618 – 63821HelicalSequence analysisAdd
BLAST
Topological domaini639 – 65315CytoplasmicSequence analysisAdd
BLAST
Transmembranei654 – 67421HelicalSequence analysisAdd
BLAST
Topological domaini675 – 73460VacuolarSequence analysisAdd
BLAST
Transmembranei735 – 75521HelicalSequence analysisAdd
BLAST
Topological domaini756 – 76813CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 768768Vacuolar basic amino acid transporter 4PRO_0000252272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621PhosphoserineCombined sources
Modified residuei99 – 991PhosphoserineCombined sources
Modified residuei106 – 1061PhosphoserineCombined sources
Modified residuei160 – 1601PhosphoserineCombined sources
Modified residuei192 – 1921PhosphoserineCombined sources
Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence analysis
Glycosylationi553 – 5531N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ04602.
PeptideAtlasiQ04602.

PTM databases

iPTMnetiQ04602.

Interactioni

Protein-protein interaction databases

BioGridi32175. 16 interactions.
DIPiDIP-8662N.
MINTiMINT-4480900.

Structurei

3D structure databases

ProteinModelPortaliQ04602.
SMRiQ04602. Positions 372-418, 736-761.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili9 – 4032Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ04602.
OrthoDBiEOG70ZZWV.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04602-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKDRQRKK LREFAKLKNR QRNLRKSVQT LKNEVQREAK VPRTSNQIAL
60 70 80 90 100
GNDKIEEINE NSPLLSAPSK QEEVSIPKAV DIDTIDAQPL HEGPKIDDSP
110 120 130 140 150
QDEVNSIKGK PADKANEDDL KPPSQHEACG NSALQSSITD FSDRSVSPLQ
160 170 180 190 200
SITSCNTPMS EHELPVSSSN SFERADDMPV VQADNQTSSS KSLHIVAPSP
210 220 230 240 250
EVPVSGDEIT SYGYGSIPQS IGDVENGLNP PYVENTSSDE LVHDLTRRRI
260 270 280 290 300
FSSCMCTYLF FIAMDSSIIL VIASKIASEF HELWRLSLVI SAYLLSNAIG
310 320 330 340 350
QLVFLKLSLI SSVKLLLCIA QFSFILGGYL SWSSAHFWTF IFARCVTGFG
360 370 380 390 400
GGSLIALKST IMNRFSQKND SRYSLSASMI TFAMGVVIGP FMMNLFDSSH
410 420 430 440 450
GSGWRNAFLI PVPFCLVNAS IMLADMYSVK STLYGRPTPT LWKRFKNTLL
460 470 480 490 500
SPDLYEILTL TLFLLCFVQV TSLDLTGLKN NTMIQALLFS VIIVCGILFF
510 520 530 540 550
LIETSDTYMN SVISMSLQGD KRLIWTMIGI SFCFAALMCI IPFGTTYFII
560 570 580 590 600
VLNLSTLQLA ERLSPFFFSI VLGYFSVSYF WKSKGQNFLL KFVLSGATLL
610 620 630 640 650
LYVALMGVSL NLPVWKQYIC LSLPFLGSSM ILTLLSNLYH EYHEQRKSPI
660 670 680 690 700
SGSIVYCFGA VGGTVGISLG GYVFHKTLIK LMHEKVMPFS KQGYLKKDLL
710 720 730 740 750
KIIKHATESS DWVHESAPKF VFQTLIECYL QACRNVFKLS TLFFTITVVA
760
IFIFNRIHCR SQNCLSLS
Length:768
Mass (Da):85,683
Last modified:November 1, 1996 - v1
Checksum:i08495E43E011C099
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48758 Genomic DNA. Translation: CAA88672.1.
BK006938 Genomic DNA. Translation: DAA11964.1.
PIRiS52684.
RefSeqiNP_010404.1. NM_001180427.1.

Genome annotation databases

EnsemblFungiiYDR119W; YDR119W; YDR119W.
GeneIDi851697.
KEGGisce:YDR119W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48758 Genomic DNA. Translation: CAA88672.1.
BK006938 Genomic DNA. Translation: DAA11964.1.
PIRiS52684.
RefSeqiNP_010404.1. NM_001180427.1.

3D structure databases

ProteinModelPortaliQ04602.
SMRiQ04602. Positions 372-418, 736-761.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32175. 16 interactions.
DIPiDIP-8662N.
MINTiMINT-4480900.

Protein family/group databases

TCDBi2.A.1.48.5. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ04602.

Proteomic databases

MaxQBiQ04602.
PeptideAtlasiQ04602.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR119W; YDR119W; YDR119W.
GeneIDi851697.
KEGGisce:YDR119W.

Organism-specific databases

EuPathDBiFungiDB:YDR119W.
SGDiS000002526. VBA4.

Phylogenomic databases

InParanoidiQ04602.
OrthoDBiEOG70ZZWV.

Enzyme and pathway databases

BioCyciYEAST:G3O-29719-MONOMER.

Miscellaneous databases

NextBioi969362.
PROiQ04602.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. "A family of basic amino acid transporters of the vacuolar membrane from Saccharomyces cerevisiae."
    Shimazu M., Sekito T., Akiyama K., Ohsumi Y., Kakinuma Y.
    J. Biol. Chem. 280:4851-4857(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION PREDICTION.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99; SER-106 AND SER-192, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-99; SER-106 AND SER-160, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVBA4_YEAST
AccessioniPrimary (citable) accession number: Q04602
Secondary accession number(s): D6VSA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: November 1, 1996
Last modified: April 13, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.