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Protein

Translation machinery-associated protein 64

Gene

TMA64

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • RNA binding Source: SGD
  • translation initiation factor activity Source: InterPro

GO - Biological processi

  • rRNA processing Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29717-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation machinery-associated protein 64
Gene namesi
Name:TMA64
Ordered Locus Names:YDR117C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR117C.
SGDiS000002524. TMA64.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Translation machinery-associated protein 64PRO_0000244640Add
BLAST

Proteomic databases

MaxQBiQ04600.
PeptideAtlasiQ04600.

PTM databases

iPTMnetiQ04600.

Interactioni

Subunit structurei

Interacts with the 40S ribosomal subunit.1 Publication

Protein-protein interaction databases

BioGridi32173. 22 interactions.
IntActiQ04600. 6 interactions.
MINTiMINT-2492923.

Structurei

3D structure databases

ProteinModelPortaliQ04600.
SMRiQ04600. Positions 1-182, 368-425, 473-556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini89 – 17082PUAPROSITE-ProRule annotationAdd
BLAST
Domaini363 – 44684SWIBAdd
BLAST
Domaini475 – 54773SUI1PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi558 – 5658Poly-Lys

Domaini

The PUA and the SUI1 domains may be involved in RNA binding.1 Publication

Sequence similaritiesi

Belongs to the eIF2D family.Curated
Contains 1 PUA domain.PROSITE-ProRule annotation
Contains 1 SUI1 domain.PROSITE-ProRule annotation
Contains 1 SWIB domain.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074865.
HOGENOMiHOG000248777.
InParanoidiQ04600.
KOiK15027.
OMAiVKMLHLF.
OrthoDBiEOG7PP5J9.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
2.30.130.10. 1 hit.
InterProiIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR003121. SWIB_MDM2_domain.
IPR001950. TIF_SUI1.
[Graphical view]
PfamiPF01253. SUI1. 1 hit.
PF02201. SWIB. 1 hit.
[Graphical view]
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF55159. SSF55159. 1 hit.
SSF88697. SSF88697. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
PS50296. SUI1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04600-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKKEPHIKA LSNLKNSERK KLLQTFQKQT NNEEYSFRTS TIKQTNFNGQ
60 70 80 90 100
KSVGTVYTDE NNTPILFKEK HKEQLFPTVY SCWEYPALLP IVLTHGFVIE
110 120 130 140 150
EHLFNGANLM ISGSIPPFDP RCKIGTLCGI ASKQAPETVL AIGIVELDLP
160 170 180 190 200
SFDKVIGETG VAVKIIHHFN DGLSKVFKMK LEPPFVLSTQ SKDNNISSKQ
210 220 230 240 250
IESSEQIKAV EKEQEDVKEA SVDVEEIAEV LDHFTVSDVD YFITRALYYT
260 270 280 290 300
LTQDKGLELP ISASNFISNH IMRNLPPIDH NEVNVKKTSW KKSAKFLKHF
310 320 330 340 350
EKEGFLKLKG KGDDLTIVGK NTDKDELKNF VPYKLGCSKS ATESRESTTS
360 370 380 390 400
KEKTSGMMYS LTLYKPFNLA KDLLKEVNLA SHTYYTSQDI RSAVSQYISV
410 420 430 440 450
KNLADTKDKG KVIMDDLLFD MVNKKKKVLN ASRIIARGEI LHPLLTNNFT
460 470 480 490 500
EFYQIFKSDD TLLFKAPMKG SLPHIKIITE MKIGRKVITR VSNFEVFQVD
510 520 530 540 550
PESLAADLRK ICSGSTTISE SQTFKCAEVQ VQGPHGQSII DHLNKLGIPS
560
KWIDFENKLK KKKRK
Length:565
Mass (Da):63,992
Last modified:November 1, 1996 - v1
Checksum:i2ED921EFABEF3739
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48758 Genomic DNA. Translation: CAA88670.1.
BK006938 Genomic DNA. Translation: DAA11962.1.
PIRiS52682.
RefSeqiNP_010402.3. NM_001180425.3.

Genome annotation databases

EnsemblFungiiYDR117C; YDR117C; YDR117C.
GeneIDi851695.
KEGGisce:YDR117C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48758 Genomic DNA. Translation: CAA88670.1.
BK006938 Genomic DNA. Translation: DAA11962.1.
PIRiS52682.
RefSeqiNP_010402.3. NM_001180425.3.

3D structure databases

ProteinModelPortaliQ04600.
SMRiQ04600. Positions 1-182, 368-425, 473-556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32173. 22 interactions.
IntActiQ04600. 6 interactions.
MINTiMINT-2492923.

PTM databases

iPTMnetiQ04600.

Proteomic databases

MaxQBiQ04600.
PeptideAtlasiQ04600.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR117C; YDR117C; YDR117C.
GeneIDi851695.
KEGGisce:YDR117C.

Organism-specific databases

EuPathDBiFungiDB:YDR117C.
SGDiS000002524. TMA64.

Phylogenomic databases

GeneTreeiENSGT00550000074865.
HOGENOMiHOG000248777.
InParanoidiQ04600.
KOiK15027.
OMAiVKMLHLF.
OrthoDBiEOG7PP5J9.

Enzyme and pathway databases

BioCyciYEAST:G3O-29717-MONOMER.

Miscellaneous databases

NextBioi969356.
PROiQ04600.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
2.30.130.10. 1 hit.
InterProiIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR003121. SWIB_MDM2_domain.
IPR001950. TIF_SUI1.
[Graphical view]
PfamiPF01253. SUI1. 1 hit.
PF02201. SWIB. 1 hit.
[Graphical view]
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF55159. SSF55159. 1 hit.
SSF88697. SSF88697. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
PS50296. SUI1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Novel predicted RNA-binding domains associated with the translation machinery."
    Aravind L., Koonin E.V.
    J. Mol. Evol. 48:291-302(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAINS.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes."
    Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J.
    Genes Dev. 20:1294-1307(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.

Entry informationi

Entry nameiTMA64_YEAST
AccessioniPrimary (citable) accession number: Q04600
Secondary accession number(s): D6VSA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3870 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.