Reviewed, UniProtKB/Swiss-Prot Q045S8 (GUAC_LACGA)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	LGAS_0389

Organism

Lactobacillus gasseri (strain ATCC 33323 / DSM 20243) [Complete proteome] [HAMAP]

Taxonomic identifier

324831 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH(3) + NADP(+) = guanosine 5'-phosphate + NADPH.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: InterPro purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	GMP reductase		PRO_0000294275
Regions
	Nucleotide binding	209 – 232	24	NADP Potential
Sites
	Active site	180	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q045S8-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: C20266C30D19BDDE	FASTA	330	36,644
	10 20 30 40 50 60 MSNYFSMEAF DYDDIQLVPN KCIIKSRKEA DTSVKFGSRT FKIPVVPANM ESVIDDDLAI 70 80 90 100 110 120 WLAENGYYYV MHRFHPEKRA NFIKMMHDKG LFASISVGIK DSEYDFIDYL AKEKIIPEYI 130 140 150 160 170 180 TIDVAHGHSD YVIKMIKYIK DKLPDTFLTA GNIATPEAVR ELENAGADAT KVGVGPGRAC 190 200 210 220 230 240 ITKLKTGFGT GGWQLAALRM CSKAARKPLI ADGGIRHNGD IAKSVRFGAS MVMIGSLFAG 250 260 270 280 290 300 HEESPGNLIT IDGKRYKQYW GSASEVQKGA YRNVEGKQML VPYRGSIKDT LREMQEDLQS 310 320 330 SISYAGGKDL SAIRVVDYVI VKSSIFDGDK

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000413 Genomic DNA. Translation: ABJ59794.1.
RefSeq	YP_814232.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4440320.
GenomeReviews	Gene locus LGAS_0389 in contig CP000413_GR.
KEGG	lga:LGAS_0389.
NMPDR	fig|1596.1.peg.1138.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q045S8.
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DHase_GMPRtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_LACGA

Accession

Primary (citable) accession number: Q045S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	November 14, 2006
Last modified:	November 25, 2008
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)