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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactobacillus gasseri (strain ATCC 33323 / DSM 20243 / JCM 1131 / NCIMB 11718 / AM63)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei576 – 5761ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLGAS324831:GHTY-459-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:LGAS_0459
OrganismiLactobacillus gasseri (strain ATCC 33323 / DSM 20243 / JCM 1131 / NCIMB 11718 / AM63)
Taxonomic identifieri324831 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000664 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Leucine--tRNA ligasePRO_1000091327Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi324831.LGAS_0459.

Structurei

3D structure databases

ProteinModelPortaliQ045L5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionAdd
BLAST
Motifi573 – 5775"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q045L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNHKTVEKK WQKYWAEHDT FKTGNDPKKK NYYALDMFPF PSGKGLHVGH
60 70 80 90 100
PEGYTATDII SRMKRAQGYN VLHPMGWDAF GLPTEQYALK TGEDPEKVTK
110 120 130 140 150
ENIANFKKQL NKLGFSYDWD REVTTSDPNY YKWTQWVFEQ MYKKGLAYEA
160 170 180 190 200
EVPVNWSPDL GTVVANEEIV DGKTERGGYP VYRRNMRQWM LKMTAYADRL
210 220 230 240 250
LEDLDDLDWP EPVKEMQRNW IGRSEGAQVT FKVKDSDKTF DVFTTRPDTL
260 270 280 290 300
FGVSYTVLAP ESKLVQEITT PEQKAAVDSY IKKIESKSDL ERTDLNKDKT
310 320 330 340 350
GVFTGAYAIN PVNGKEVPVW ISDYVLASYG TGAVMAVPAH DDRDYAFATK
360 370 380 390 400
FGLPINRVIE GGDLEKEAFT GNGKHINSDF LDGLDNEEAK KRMIEWLEDH
410 420 430 440 450
NAGQKKVNYK LRDWDFSRQR YWGEPIPVIH WEDGTTSLVP EDELPLRLPH
460 470 480 490 500
ATDIKPSGTP ESPLANLTDW VNVVDKNGRK GKRETNTMPN WAGSSWYYLR
510 520 530 540 550
YIDPHNDKEL ADYDLLKKWL PVDLYIGGAE HAVRHLLYAR FWHKVLYDLG
560 570 580 590 600
VVPTKEPFQR LYNQGLILKN HEKMSKSKGN VVNPDDVIDE YGADSLRMYE
610 620 630 640 650
MFMGPLDASI DWDDNGPAST KKFLDRVWRL FVNDLDLKAI PQERIVDKND
660 670 680 690 700
GELDKVYAET VKKVTEDFDA LHFNTAISQM MVFINAAQKA KTIPREYAEG
710 720 730 740 750
FVKLLAPVAP HMMEEIWQVF GHDESISYAK WPEYDPAKLV ESTVEIMVQV
760 770 780 790 800
NGKLRGKFKA AKDSAKDALE KEALSLDHVQ KFLDGKDVKK VIVIPNKIVN

IVAK
Length:804
Mass (Da):92,302
Last modified:November 14, 2006 - v1
Checksum:iC0F075BFFE9FFE06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000413 Genomic DNA. Translation: ABJ59857.1.
RefSeqiYP_814295.1. NC_008530.1.

Genome annotation databases

EnsemblBacteriaiABJ59857; ABJ59857; LGAS_0459.
KEGGilga:LGAS_0459.
PATRICi22229062. VBILacGas62225_0446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000413 Genomic DNA. Translation: ABJ59857.1.
RefSeqiYP_814295.1. NC_008530.1.

3D structure databases

ProteinModelPortaliQ045L5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi324831.LGAS_0459.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ59857; ABJ59857; LGAS_0459.
KEGGilga:LGAS_0459.
PATRICi22229062. VBILacGas62225_0446.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciLGAS324831:GHTY-459-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33323 / DSM 20243 / JCM 1131 / NCIMB 11718 / AM63.

Entry informationi

Entry nameiSYL_LACGA
AccessioniPrimary (citable) accession number: Q045L5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 14, 2006
Last modified: April 1, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.