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Q045L5 (SYL_LACGA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LGAS_0459
OrganismLactobacillus gasseri (strain ATCC 33323 / DSM 20243) [Complete proteome] [HAMAP]
Taxonomic identifier324831 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091327

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif573 – 5775"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5761ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q045L5 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: C0F075BFFE9FFE06

FASTA80492,302
        10         20         30         40         50         60 
MYNHKTVEKK WQKYWAEHDT FKTGNDPKKK NYYALDMFPF PSGKGLHVGH PEGYTATDII 

        70         80         90        100        110        120 
SRMKRAQGYN VLHPMGWDAF GLPTEQYALK TGEDPEKVTK ENIANFKKQL NKLGFSYDWD 

       130        140        150        160        170        180 
REVTTSDPNY YKWTQWVFEQ MYKKGLAYEA EVPVNWSPDL GTVVANEEIV DGKTERGGYP 

       190        200        210        220        230        240 
VYRRNMRQWM LKMTAYADRL LEDLDDLDWP EPVKEMQRNW IGRSEGAQVT FKVKDSDKTF 

       250        260        270        280        290        300 
DVFTTRPDTL FGVSYTVLAP ESKLVQEITT PEQKAAVDSY IKKIESKSDL ERTDLNKDKT 

       310        320        330        340        350        360 
GVFTGAYAIN PVNGKEVPVW ISDYVLASYG TGAVMAVPAH DDRDYAFATK FGLPINRVIE 

       370        380        390        400        410        420 
GGDLEKEAFT GNGKHINSDF LDGLDNEEAK KRMIEWLEDH NAGQKKVNYK LRDWDFSRQR 

       430        440        450        460        470        480 
YWGEPIPVIH WEDGTTSLVP EDELPLRLPH ATDIKPSGTP ESPLANLTDW VNVVDKNGRK 

       490        500        510        520        530        540 
GKRETNTMPN WAGSSWYYLR YIDPHNDKEL ADYDLLKKWL PVDLYIGGAE HAVRHLLYAR 

       550        560        570        580        590        600 
FWHKVLYDLG VVPTKEPFQR LYNQGLILKN HEKMSKSKGN VVNPDDVIDE YGADSLRMYE 

       610        620        630        640        650        660 
MFMGPLDASI DWDDNGPAST KKFLDRVWRL FVNDLDLKAI PQERIVDKND GELDKVYAET 

       670        680        690        700        710        720 
VKKVTEDFDA LHFNTAISQM MVFINAAQKA KTIPREYAEG FVKLLAPVAP HMMEEIWQVF 

       730        740        750        760        770        780 
GHDESISYAK WPEYDPAKLV ESTVEIMVQV NGKLRGKFKA AKDSAKDALE KEALSLDHVQ 

       790        800 
KFLDGKDVKK VIVIPNKIVN IVAK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000413 Genomic DNA. Translation: ABJ59857.1.
RefSeqYP_814295.1. NC_008530.1.

3D structure databases

ProteinModelPortalQ045L5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING324831.LGAS_0459.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ59857; ABJ59857; LGAS_0459.
GeneID4440355.
KEGGlga:LGAS_0459.
PATRIC22229062. VBILacGas62225_0446.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycLGAS324831:GHTY-459-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACGA
AccessionPrimary (citable) accession number: Q045L5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 14, 2006
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries