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Q04552 (CP6B1_PAPPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 6B1

EC=1.14.14.1
Alternative name(s):
CYP6B1v1/CYP6B1v2/CYP6B1v3
CYPVIB1
Gene names
Name:CYP6B1
OrganismPapilio polyxenes (Black swallowtail butterfly)
Taxonomic identifier7146 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPapilionoideaPapilionidaePapilioninaePapilio

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Midgut microsome.

Induction

By xanthotoxin, a secondary metabolite abundant in the host plants of this specialized herbivore.

Polymorphism

The sequence shown is that of 6B1-1, 6B1-2 seems to differ in 9 positions and is probably an allele.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityPolymorphism
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaromatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Cytochrome P450 6B1
PRO_0000051893

Sites

Metal binding4431Iron (heme axial ligand) By similarity

Natural variations

Natural variant241N → D in 6B1-2 and 6B1-3.
Natural variant155 – 1562NS → KC in 6B1-2 and 6B1-3.
Natural variant2431K → R in 6B1-2.
Natural variant2851A → S in 6B1-2.
Natural variant2931I → V in 6B1-2.
Natural variant4581M → V in 6B1-2.
Natural variant4751P → E in 6B1-2.
Natural variant4951L → I in 6B1-2.

Sequences

Sequence LengthMass (Da)Tools
Q04552 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 043A849CA0990153

FASTA49857,484
        10         20         30         40         50         60 
MLYLLALVTV LAGLLHYYFT RTFNYWKKRN VAGPKPVPFF GNLKDSVLRR KPQVMVYKSI 

        70         80         90        100        110        120 
YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VLIKDFESFA DRGVEFSLDG LGANIFHADG 

       130        140        150        160        170        180 
DRWRSLRNRF TPLFTSGKLK SMLPLMSQVG DRFINSIDEV SQTQPEQSIH NLVQKFTMTN 

       190        200        210        220        230        240 
IAACVFGLNL DEGMLKTLED LDKHIFTVNY SAELDMMYPG ILKKLNGSLF PKVVSKFFDN 

       250        260        270        280        290        300 
LTKNVLEMRK GTPSYQKDMI DLIQELREKK TLELSRKHEN EDVKALELTD GVISAQMFIF 

       310        320        330        340        350        360 
YMAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLS EMTYLSKVFD 

       370        380        390        400        410        420 
ETLRKYPVAD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW GIQNDPKYYP NPEKFDPERF 

       430        440        450        460        470        480 
NPENVKDRHP CAYLPFSAGP RNCLGMRFAK WQSEVCIMKV LSKYRVEPSM KSSGPFKFDP 

       490 
MRLFALPKGG IYVNLVRR 

« Hide

References

[1]"A host-inducible cytochrome P-450 from a host-specific caterpillar: molecular cloning and evolution."
Cohen M.B., Schuler M.A., Berenbaum M.R.
Proc. Natl. Acad. Sci. U.S.A. 89:10920-10924(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-25 AND 401-406.
Tissue: Midgut.
[2]"Transcriptional regulation of the Papilio polyxenes CYP6B1 gene."
Prapaipong H.H., Berenbaum M.M., Schuler M.M.
Nucleic Acids Res. 22:3210-3217(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CYP6B1V3).
Tissue: Midgut.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80828 mRNA. Translation: AAA29789.1.
M83117 mRNA. Translation: AAA29790.1.
Z29624 Genomic DNA. Translation: CAA82732.1.
U05037 Unassigned DNA. Translation: AAA16154.1.
PIRS48058.

3D structure databases

ProteinModelPortalQ04552.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP6B1_PAPPO
AccessionPrimary (citable) accession number: Q04552
Secondary accession number(s): Q04553, Q27878
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: December 11, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families