Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q04552

- CP6B1_PAPPO

UniProt

Q04552 - CP6B1_PAPPO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Cytochrome P450 6B1

Gene

CYP6B1

Organism
Papilio polyxenes (Black swallowtail butterfly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi443 – 4431Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 6B1 (EC:1.14.14.1)
Alternative name(s):
CYP6B1v1/CYP6B1v2/CYP6B1v3
CYPVIB1
Gene namesi
Name:CYP6B1
OrganismiPapilio polyxenes (Black swallowtail butterfly)
Taxonomic identifieri7146 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPapilionoideaPapilionidaePapilioninaePapilio

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Cytochrome P450 6B1PRO_0000051893Add
BLAST

Expressioni

Tissue specificityi

Midgut microsome.

Inductioni

By xanthotoxin, a secondary metabolite abundant in the host plants of this specialized herbivore.

Structurei

3D structure databases

ProteinModelPortaliQ04552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04552-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLYLLALVTV LAGLLHYYFT RTFNYWKKRN VAGPKPVPFF GNLKDSVLRR
60 70 80 90 100
KPQVMVYKSI YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VLIKDFESFA
110 120 130 140 150
DRGVEFSLDG LGANIFHADG DRWRSLRNRF TPLFTSGKLK SMLPLMSQVG
160 170 180 190 200
DRFINSIDEV SQTQPEQSIH NLVQKFTMTN IAACVFGLNL DEGMLKTLED
210 220 230 240 250
LDKHIFTVNY SAELDMMYPG ILKKLNGSLF PKVVSKFFDN LTKNVLEMRK
260 270 280 290 300
GTPSYQKDMI DLIQELREKK TLELSRKHEN EDVKALELTD GVISAQMFIF
310 320 330 340 350
YMAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLS
360 370 380 390 400
EMTYLSKVFD ETLRKYPVAD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW
410 420 430 440 450
GIQNDPKYYP NPEKFDPERF NPENVKDRHP CAYLPFSAGP RNCLGMRFAK
460 470 480 490
WQSEVCIMKV LSKYRVEPSM KSSGPFKFDP MRLFALPKGG IYVNLVRR
Length:498
Mass (Da):57,484
Last modified:October 1, 1993 - v1
Checksum:i043A849CA0990153
GO

Polymorphismi

The sequence shown is that of 6B1-1, 6B1-2 seems to differ in 9 positions and is probably an allele.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti24 – 241N → D in 6B1-2 and 6B1-3.
Natural varianti155 – 1562NS → KC in 6B1-2 and 6B1-3.
Natural varianti243 – 2431K → R in 6B1-2.
Natural varianti285 – 2851A → S in 6B1-2.
Natural varianti293 – 2931I → V in 6B1-2.
Natural varianti458 – 4581M → V in 6B1-2.
Natural varianti475 – 4751P → E in 6B1-2.
Natural varianti495 – 4951L → I in 6B1-2.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80828 mRNA. Translation: AAA29789.1.
M83117 mRNA. Translation: AAA29790.1.
Z29624 Genomic DNA. Translation: CAA82732.1.
U05037 Unassigned DNA. Translation: AAA16154.1.
PIRiS48058.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80828 mRNA. Translation: AAA29789.1 .
M83117 mRNA. Translation: AAA29790.1 .
Z29624 Genomic DNA. Translation: CAA82732.1 .
U05037 Unassigned DNA. Translation: AAA16154.1 .
PIRi S48058.

3D structure databases

ProteinModelPortali Q04552.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A host-inducible cytochrome P-450 from a host-specific caterpillar: molecular cloning and evolution."
    Cohen M.B., Schuler M.A., Berenbaum M.R.
    Proc. Natl. Acad. Sci. U.S.A. 89:10920-10924(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-25 AND 401-406.
    Tissue: Midgut.
  2. "Transcriptional regulation of the Papilio polyxenes CYP6B1 gene."
    Prapaipong H.H., Berenbaum M.M., Schuler M.M.
    Nucleic Acids Res. 22:3210-3217(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CYP6B1V3).
    Tissue: Midgut.

Entry informationi

Entry nameiCP6B1_PAPPO
AccessioniPrimary (citable) accession number: Q04552
Secondary accession number(s): Q04553, Q27878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3