Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytochrome P450 6B1

Gene

CYP6B1

Organism
Papilio polyxenes (Black swallowtail butterfly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi443Iron (heme axial ligand)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 6B1 (EC:1.14.14.1)
Alternative name(s):
CYP6B1v1/CYP6B1v2/CYP6B1v3
CYPVIB1
Gene namesi
Name:CYP6B1
OrganismiPapilio polyxenes (Black swallowtail butterfly)
Taxonomic identifieri7146 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaLepidopteraGlossataDitrysiaPapilionoideaPapilionidaePapilioninaePapilio

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000518931 – 498Cytochrome P450 6B1Add BLAST498

Expressioni

Tissue specificityi

Midgut microsome.

Inductioni

By xanthotoxin, a secondary metabolite abundant in the host plants of this specialized herbivore.

Structurei

3D structure databases

ProteinModelPortaliQ04552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYLLALVTV LAGLLHYYFT RTFNYWKKRN VAGPKPVPFF GNLKDSVLRR
60 70 80 90 100
KPQVMVYKSI YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VLIKDFESFA
110 120 130 140 150
DRGVEFSLDG LGANIFHADG DRWRSLRNRF TPLFTSGKLK SMLPLMSQVG
160 170 180 190 200
DRFINSIDEV SQTQPEQSIH NLVQKFTMTN IAACVFGLNL DEGMLKTLED
210 220 230 240 250
LDKHIFTVNY SAELDMMYPG ILKKLNGSLF PKVVSKFFDN LTKNVLEMRK
260 270 280 290 300
GTPSYQKDMI DLIQELREKK TLELSRKHEN EDVKALELTD GVISAQMFIF
310 320 330 340 350
YMAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLS
360 370 380 390 400
EMTYLSKVFD ETLRKYPVAD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW
410 420 430 440 450
GIQNDPKYYP NPEKFDPERF NPENVKDRHP CAYLPFSAGP RNCLGMRFAK
460 470 480 490
WQSEVCIMKV LSKYRVEPSM KSSGPFKFDP MRLFALPKGG IYVNLVRR
Length:498
Mass (Da):57,484
Last modified:October 1, 1993 - v1
Checksum:i043A849CA0990153
GO

Polymorphismi

The sequence shown is that of 6B1-1, 6B1-2 seems to differ in 9 positions and is probably an allele.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti24N → D in 6B1-2 and 6B1-3. 1
Natural varianti155 – 156NS → KC in 6B1-2 and 6B1-3. 2
Natural varianti243K → R in 6B1-2. 1
Natural varianti285A → S in 6B1-2. 1
Natural varianti293I → V in 6B1-2. 1
Natural varianti458M → V in 6B1-2. 1
Natural varianti475P → E in 6B1-2. 1
Natural varianti495L → I in 6B1-2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80828 mRNA. Translation: AAA29789.1.
M83117 mRNA. Translation: AAA29790.1.
Z29624 Genomic DNA. Translation: CAA82732.1.
U05037 Unassigned DNA. Translation: AAA16154.1.
PIRiS48058.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCP6B1_PAPPO
AccessioniPrimary (citable) accession number: Q04552
Secondary accession number(s): Q04553, Q27878
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: August 30, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families