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Q04552

- CP6B1_PAPPO

UniProt

Q04552 - CP6B1_PAPPO

Protein

Cytochrome P450 6B1

Gene

CYP6B1

Organism
Papilio polyxenes (Black swallowtail butterfly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi443 – 4431Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 6B1 (EC:1.14.14.1)
    Alternative name(s):
    CYP6B1v1/CYP6B1v2/CYP6B1v3
    CYPVIB1
    Gene namesi
    Name:CYP6B1
    OrganismiPapilio polyxenes (Black swallowtail butterfly)
    Taxonomic identifieri7146 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPapilionoideaPapilionidaePapilioninaePapilio

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 498498Cytochrome P450 6B1PRO_0000051893Add
    BLAST

    Expressioni

    Tissue specificityi

    Midgut microsome.

    Inductioni

    By xanthotoxin, a secondary metabolite abundant in the host plants of this specialized herbivore.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04552.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04552-1 [UniParc]FASTAAdd to Basket

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    MLYLLALVTV LAGLLHYYFT RTFNYWKKRN VAGPKPVPFF GNLKDSVLRR    50
    KPQVMVYKSI YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VLIKDFESFA 100
    DRGVEFSLDG LGANIFHADG DRWRSLRNRF TPLFTSGKLK SMLPLMSQVG 150
    DRFINSIDEV SQTQPEQSIH NLVQKFTMTN IAACVFGLNL DEGMLKTLED 200
    LDKHIFTVNY SAELDMMYPG ILKKLNGSLF PKVVSKFFDN LTKNVLEMRK 250
    GTPSYQKDMI DLIQELREKK TLELSRKHEN EDVKALELTD GVISAQMFIF 300
    YMAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLS 350
    EMTYLSKVFD ETLRKYPVAD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW 400
    GIQNDPKYYP NPEKFDPERF NPENVKDRHP CAYLPFSAGP RNCLGMRFAK 450
    WQSEVCIMKV LSKYRVEPSM KSSGPFKFDP MRLFALPKGG IYVNLVRR 498
    Length:498
    Mass (Da):57,484
    Last modified:October 1, 1993 - v1
    Checksum:i043A849CA0990153
    GO

    Polymorphismi

    The sequence shown is that of 6B1-1, 6B1-2 seems to differ in 9 positions and is probably an allele.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti24 – 241N → D in 6B1-2 and 6B1-3.
    Natural varianti155 – 1562NS → KC in 6B1-2 and 6B1-3.
    Natural varianti243 – 2431K → R in 6B1-2.
    Natural varianti285 – 2851A → S in 6B1-2.
    Natural varianti293 – 2931I → V in 6B1-2.
    Natural varianti458 – 4581M → V in 6B1-2.
    Natural varianti475 – 4751P → E in 6B1-2.
    Natural varianti495 – 4951L → I in 6B1-2.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80828 mRNA. Translation: AAA29789.1.
    M83117 mRNA. Translation: AAA29790.1.
    Z29624 Genomic DNA. Translation: CAA82732.1.
    U05037 Unassigned DNA. Translation: AAA16154.1.
    PIRiS48058.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80828 mRNA. Translation: AAA29789.1 .
    M83117 mRNA. Translation: AAA29790.1 .
    Z29624 Genomic DNA. Translation: CAA82732.1 .
    U05037 Unassigned DNA. Translation: AAA16154.1 .
    PIRi S48058.

    3D structure databases

    ProteinModelPortali Q04552.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A host-inducible cytochrome P-450 from a host-specific caterpillar: molecular cloning and evolution."
      Cohen M.B., Schuler M.A., Berenbaum M.R.
      Proc. Natl. Acad. Sci. U.S.A. 89:10920-10924(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-25 AND 401-406.
      Tissue: Midgut.
    2. "Transcriptional regulation of the Papilio polyxenes CYP6B1 gene."
      Prapaipong H.H., Berenbaum M.M., Schuler M.M.
      Nucleic Acids Res. 22:3210-3217(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CYP6B1V3).
      Tissue: Midgut.

    Entry informationi

    Entry nameiCP6B1_PAPPO
    AccessioniPrimary (citable) accession number: Q04552
    Secondary accession number(s): Q04553, Q27878
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3