Q04552 (CP6B1_PAPPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 6B1
EC=1.14.14.1

Alternative name(s):
CYP6B1v1/CYP6B1v2/CYP6B1v3
CYPVIB1

Gene names

Name:

CYP6B1

Organism

Papilio polyxenes (Black swallowtail butterfly)

Taxonomic identifier

7146 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Amphiesmenoptera › Lepidoptera › Glossata › Neolepidoptera › Heteroneura › Ditrysia › Obtectomera › Papilionoidea › Papilionidae › Papilioninae › Papilionini › Papilio

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Enables the insect to feed on furanocoumarin-producing plants and evolved as an adaptation for detoxification of xanthotoxin and other furanocoumarins.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Midgut microsome.
Induction	By xanthotoxin, a secondary metabolite abundant in the host plants of this specialized herbivore.
Polymorphism	The sequence shown is that of 6B1-1, 6B1-2 seems to differ in 9 positions and is probably an allele.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Cytochrome P450 6B1		PRO_0000051893
Sites
	Metal binding	443	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	24	1	N → D in 6B1-2 and 6B1-3.
	Natural variant	155 – 156	2	NS → KC in 6B1-2 and 6B1-3.
	Natural variant	243	1	K → R in 6B1-2.
	Natural variant	285	1	A → S in 6B1-2.
	Natural variant	293	1	I → V in 6B1-2.
	Natural variant	458	1	M → V in 6B1-2.
	Natural variant	475	1	P → E in 6B1-2.
	Natural variant	495	1	L → I in 6B1-2.

Sequences

	Sequence		Length	Mass (Da)
	Q04552 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 043A849CA0990153	FASTA	498	57,484
	10 20 30 40 50 60 MLYLLALVTV LAGLLHYYFT RTFNYWKKRN VAGPKPVPFF GNLKDSVLRR KPQVMVYKSI 70 80 90 100 110 120 YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VLIKDFESFA DRGVEFSLDG LGANIFHADG 130 140 150 160 170 180 DRWRSLRNRF TPLFTSGKLK SMLPLMSQVG DRFINSIDEV SQTQPEQSIH NLVQKFTMTN 190 200 210 220 230 240 IAACVFGLNL DEGMLKTLED LDKHIFTVNY SAELDMMYPG ILKKLNGSLF PKVVSKFFDN 250 260 270 280 290 300 LTKNVLEMRK GTPSYQKDMI DLIQELREKK TLELSRKHEN EDVKALELTD GVISAQMFIF 310 320 330 340 350 360 YMAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLS EMTYLSKVFD 370 380 390 400 410 420 ETLRKYPVAD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW GIQNDPKYYP NPEKFDPERF 430 440 450 460 470 480 NPENVKDRHP CAYLPFSAGP RNCLGMRFAK WQSEVCIMKV LSKYRVEPSM KSSGPFKFDP 490 MRLFALPKGG IYVNLVRR

References

[1]	"A host-inducible cytochrome P-450 from a host-specific caterpillar: molecular cloning and evolution." Cohen M.B., Schuler M.A., Berenbaum M.R. Proc. Natl. Acad. Sci. U.S.A. 89:10920-10924(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-25 AND 401-406. Tissue: Midgut.
[2]	"Transcriptional regulation of the Papilio polyxenes CYP6B1 gene." Prapaipong H.H., Berenbaum M.M., Schuler M.M. Nucleic Acids Res. 22:3210-3217(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CYP6B1V3). Tissue: Midgut.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80828 mRNA. Translation: AAA29789.1. M83117 mRNA. Translation: AAA29790.1. Z29624 Genomic DNA. Translation: CAA82732.1. U05037 Unassigned DNA. Translation: AAA16154.1.
PIR	S48058.
3D structure databases
ProteinModelPortal	Q04552.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP6B1_PAPPO

Accession

Primary (citable) accession number: Q04552
Secondary accession number(s): Q04553, Q27878

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)