Q04549 (ARP10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Actin-like protein ARP10

Gene names

Name:	ARP10
Ordered Locus Names:	YDR106W
ORF Names:	YD9727.02, YD8557.15

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pointed-end-associated component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding By similarity. The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint. May regulate the association of the dynactin complex with the plasma membrane. Ref.5
Subunit structure	Self-associates. Component of the dynactin complex composed of at least ARP1, JNM1, NIP100 and ARP10. Dynactin comprises a short rod of the ARP1 filament attached to ARP10 at its pointed-end and probably associated with the capping protein at its barbed-end. The rod is implicated in dynein cargo binding. A sidearm formed by NIP100 projects from the ARP1 filament and is implicated in motor binding By similarity. Interacts with ARP1 and JNM1. Ref.5
Subcellular location	Cytoplasm › cytoskeleton Probable.
Sequence similarities	Belongs to the actin family. ARP10 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Dynein Microtubule
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitosis Inferred from genetic interaction Ref.5. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD dynein complex Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	motor activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARP1	P38696	5	EBI-2977,EBI-2920
JNM1	P36224	3	EBI-2977,EBI-9415

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Actin-like protein ARP10		PRO_0000089130

Sequences

	Sequence		Length	Mass (Da)
	Q04549 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C2EDD2B6D4BE48BD	FASTA	284	33,018
	10 20 30 40 50 60 MSNTIVIVYL GANRIEIGRS ADACPQEIIA WKTGSINEKN REELKKIFEH YFQICNILGN 70 80 90 100 110 120 REVQVLILED IFISVVEKRI ICSILFKEFD CAHVSFVPRA IVHCLSCNTR NAIVIDIGTN 130 140 150 160 170 180 YTTCVPIFDL RPLQQFIKYS KRGKRQVESR IPLPGSLYMP IFFDEEYNSK NCEVDETPVI 190 200 210 220 230 240 NLVKNIVESL PIDLRRPLRE NIIIVNIEEA YETVIRNLFK LKMDTSKIQF PKNYWQAGSA 250 260 270 280 CAKILLHYKG SNIVGIERDE FYNNPHIAPD WFDYYFRTGV KRLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Who's who among the Saccharomyces cerevisiae actin-related proteins? A classification and nomenclature proposal for a large family." Poch O., Winsor B. Yeast 13:1053-1058(1997) [PubMed: 9290209] [Abstract] Cited for: GENE NAME.
[5]	"Arp10p is a pointed-end-associated component of yeast dynactin." Clark S.W., Rose M.D. Mol. Biol. Cell 17:738-748(2006) [PubMed: 16291862] [Abstract] Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH ARP1 AND JNM1, IDENTIFICATION IN THE DYNACTIN COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48758 Genomic DNA. Translation: CAA88660.1. Z47746 Genomic DNA. Translation: CAA87682.1. AY557682 Genomic DNA. Translation: AAS56008.1. BK006938 Genomic DNA. Translation: DAA11952.1.
PIR	S52672.
RefSeq	NP_010391.1. NM_001180414.1.
3D structure databases
ProteinModelPortal	Q04549.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1204N.
IntAct	Q04549. 7 interactions.
MINT	MINT-389749.
STRING	Q04549.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR106W; YDR106W; YDR106W.
GeneID	851683.
KEGG	sce:YDR106W.
NMPDR	fig|4932.3.peg.1139.
Organism-specific databases
CYGD	YDR106w.
SGD	S000002513. ARP10.
Phylogenomic databases
eggNOG	fuNOG16110.
OMA	VENCIVE.
OrthoDB	EOG47SWQN.
Gene expression databases
ArrayExpress	Q04549.
Genevestigator	Q04549.
GermOnline	YDR106W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004000. Actin-like. [Graphical view]
PANTHER	PTHR11937. Actin_like. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969324.

Entry information

Entry name

ARP10_YEAST

Accession

Primary (citable) accession number: Q04549
Secondary accession number(s): D6VS92, Q03869

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program