Q04545 (TDA9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable transcription factor TDA9

Alternative name(s):
Topoisomerase I damage affected protein 9

Gene names

Name:	TDA9
Ordered Locus Names:	YML081W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1251 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-binding protein that acts probably as a transcription factor. Ref.7
Subcellular location	Nucleus Ref.3.
Disruption phenotype	Leads to cell death when overexpressing the camptothecin mimetic TOP1-T(722)A mutant. Ref.9
Miscellaneous	Present with 672 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the RSF2/TDA9 family. Contains 2 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.3. Source: SGD
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 16785442Ref.7PubMed 19158363. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1251	1251	Probable transcription factor TDA9		PRO_0000046864
Regions
	Zinc finger	61 – 83	23	C2H2-type 1
	Zinc finger	89 – 112	24	C2H2-type 2
Amino acid modifications
	Modified residue	180	1	Phosphoserine Ref.8
	Modified residue	270	1	Phosphothreonine Ref.8
	Modified residue	527	1	Phosphoserine Ref.5 Ref.6 Ref.8
	Modified residue	603	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q04545 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 612F064177D6FF3B	FASTA	1,251	141,465
	10 20 30 40 50 60 MSSEEFKGLP IKRDISSTIY ADRPPALSAP PCVGATGNDK IQVLPIPKKS RTIKTDKPRP 70 80 90 100 110 120 FLCHICTRGF VRQEHLKRHQ RAHTNEKPFL CVFCGRCFAR RDLVLRHQHK LHSALVSKES 130 140 150 160 170 180 INSKDKTEID AINDKNIIQI QGNKQTILPT PSNPLAKTAV QLKKAAKEKK NGKQGKLDLS 190 200 210 220 230 240 PSYGANNHST DVSPSVGNSS TPAVIEETDS SSHFPLPDTN IPTKSKRHAS FSASSAFTYS 250 260 270 280 290 300 SDNFQKLHQQ AKSDFDELQE SIPHQVGFST PQLTAQQLIE NAIESGVVDL ETLDLPPFLS 310 320 330 340 350 360 LDGLPPASSS AAVAASEQID ICPSSATDTI SGANSTPNQA ATAPPFQLPI ARESSSLFLA 370 380 390 400 410 420 NTPYLSDFLT MGSSYGGSGG FAKSITADPS LDYFNYKNHS HPDSRHNNSS SGINYSNNKN 430 440 450 460 470 480 NNESIEKSQN NSNVINETID HTDIHAHHAD AHDDSFIESE EWLSKFIMDS QIDNDLKLNI 490 500 510 520 530 540 NHFNDIGFNN LHPQNPTTHS EPRNMHNENR DMHRSASKFQ SVSENISPRE QMSLFKTKQN 550 560 570 580 590 600 KAISKFLSDE KIPSTASPSS SASPVQFDKK NVDINEFLLD ESVSNLFTTR QIDLFKKNVN 610 620 630 640 650 660 LYSPLFQNQK DAVSSTSLTP SLTTQTATTQ SGPGWTDSSQ KLTFFTEQLR NLIIKENNLK 670 680 690 700 710 720 SNLFPTVDEL NHYVDLYQVE FHKYFPFIHL YSIIPSSENY PLVISISMIG ALYGFHSTHA 730 740 750 760 770 780 LLLSKIARTR VRMFLENTRS NHDKTPIWLM QSLVLLTFTS IFSNDMNAFR TVNTQIMILV 790 800 810 820 830 840 QLIKISKLNY PLENFIKPPI ESDHVLEYQD NPAVLNQFKA QYNTREQINR NFKYFILAQS 850 860 870 880 890 900 RIRICHIVLL ISNLFKSLVD FDCCFHSIDL KCGVPCYNEV LFFCENSRTW NENLTRFNIV 910 920 930 940 950 960 LDSKFSLIEV SNGESNYEKC LMYLSNGNPY LYKNAKISFK TLLSLLISIH EKINIERDAL 970 980 990 1000 1010 1020 KDSYESDFHA KNVQWRMHSR PLVATMLKHW ELLYIKNGGI LALSDENLPI INTNPSFRLI 1030 1040 1050 1060 1070 1080 IPLYFFAKLR KCLDIAPTLR CIWNQDWNSM NSSLEKVCYE RESLREATEY AVSVITFWID 1090 1100 1110 1120 1130 1140 TVSVMKGKST QTPIFTITCI FVSILVIAGY MRRLEDFAQN KNSDCMIGSL KSTDRILWLK 1150 1160 1170 1180 1190 1200 AFKTLKRIES HLSEREYKLQ TFAEFLRVPD NGSLDIESLD SSLIENTLNS HDVTNQALDI 1210 1220 1230 1240 1250 ITRTRLSSRT LYCGARILGD TPVWPVSLLF AHALQSRAIY NINHRKSVNS V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527, MASS SPECTROMETRY. Strain: YAL6B.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527, MASS SPECTROMETRY. Strain: ADR376.
[7]	"A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters." Badis G., Chan E.T., van Bakel H., Pena-Castillo L., Tillo D., Tsui K., Carlson C.D., Gossett A.J., Hasinoff M.J., Warren C.L., Gebbia M., Talukder S., Yang A., Mnaimneh S., Terterov D., Coburn D., Li Yeo A., Yeo Z.X. , Clarke N.D., Lieb J.D., Ansari A.Z., Nislow C., Hughes T.R. Mol. Cell 32:878-887(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING, PREDICTION OF FUNCTION.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180; THR-270; SER-527 AND SER-603, MASS SPECTROMETRY.
[9]	"Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage." Reid R.J., Gonzalez-Barrera S., Sunjevaric I., Alvaro D., Ciccone S., Wagner M., Rothstein R. Genome Res. 21:477-486(2011) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46660 Genomic DNA. Translation: CAA86657.1. Z46373 Genomic DNA. Translation: CAA86497.1. BK006946 Genomic DNA. Translation: DAA09816.1.
PIR	S49645.
RefSeq	NP_013630.1. NM_001182440.1.
3D structure databases
ProteinModelPortal	Q04545.
SMR	Q04545. Positions 59-114.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q04545. 2 interactions.
Proteomic databases
PaxDb	Q04545.
PeptideAtlas	Q04545.
PRIDE	Q04545.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML081W; YML081W; YML081W.
GeneID	854894.
KEGG	sce:YML081W.
Organism-specific databases
CYGD	YML081w.
SGD	S000004546. TDA9.
Phylogenomic databases
eggNOG	COG5048.
GeneTree	ENSGT00530000068350.
OMA	DYFIMAQ.
OrthoDB	EOG4TJ027.
Gene expression databases
Genevestigator	Q04545.
GermOnline	YML081W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.160.60. 2 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 2 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 2 hits. PS50157. ZINC_FINGER_C2H2_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	977858.

Entry information

Entry name

TDA9_YEAST

Accession

Primary (citable) accession number: Q04545
Secondary accession number(s): D6W0K2, Q03621

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program