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Protein

Ribulose-phosphate 3-epimerase, plasmid

Gene

cbbEP

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.By similarity

Catalytic activityi

D-ribulose 5-phosphate = D-xylulose 5-phosphate.

Cofactori

Co2+By similarity, Fe2+By similarity, Mn2+By similarity, Zn2+By similarityNote: Binds 1 divalent metal cation per subunit. Active with Co(2+), Fe(2+), Mn(2+) and Zn2+.By similarity

Pathway:iCalvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211SubstrateBy similarity
Metal bindingi46 – 461Divalent metal cationBy similarity
Active sitei48 – 481Proton acceptorBy similarity
Metal bindingi48 – 481Divalent metal cationBy similarity
Metal bindingi79 – 791Divalent metal cationBy similarity
Binding sitei79 – 791SubstrateBy similarity
Active sitei192 – 1921Proton donorBy similarity
Metal bindingi192 – 1921Divalent metal cationBy similarity
Binding sitei194 – 1941Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Calvin cycle, Carbohydrate metabolism

Keywords - Ligandi

Cobalt, Iron, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCNEC381666:GJUJ-6697-MONOMER.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose-phosphate 3-epimerase, plasmid (EC:5.1.3.1)
Alternative name(s):
Pentose-5-phosphate 3-epimerase
Short name:
PPE
R5P3E
Gene namesi
Name:cbbEP
Synonyms:cfxE
Ordered Locus Names:PHG423
Encoded oniPlasmid megaplasmid pHG10 Publication
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000008210 Componenti: Plasmid megaplasmid pHG1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 241241Ribulose-phosphate 3-epimerase, plasmidPRO_0000171557Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ04539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni155 – 1584Substrate bindingBy similarity
Regioni214 – 2152Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0036.
HOGENOMiHOG000259347.
KOiK01783.
OMAiHATEPNT.
OrthoDBiEOG67HK17.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR026019. Ribul_P_3_epim.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
PIRSFiPIRSF001461. RPE. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHATEPNTGH GSQRAIRLAP SILSADFARL GEEVCAIEAG GADLVHFDVM
60 70 80 90 100
DNHYVSNLTI GPLVCEAIRP LVSIPIDVHL MVEPVDALIP MFAKAGANLI
110 120 130 140 150
SFHPEASRHV DRTIGLIRDH GCKAGLVLNP ATPLSWLDHT LDKLDLVLLM
160 170 180 190 200
SVNPGFGGQA FIPGVLDKVR QARARIDRQV AAGGRPVWLE IDGGVKADNI
210 220 230 240
TEIARAGADT FVAGSAVFGA PDADGGYRGI LHRLREAATI T
Length:241
Mass (Da):25,594
Last modified:February 1, 1995 - v1
Checksum:i6FACA5B81D6734CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64172 Genomic DNA. Translation: AAA98231.1.
AY305378 Genomic DNA. Translation: AAP86172.1.
RefSeqiWP_011154335.1. NC_005241.1.

Genome annotation databases

EnsemblBacteriaiAAP86172; AAP86172; PHG423.
GeneIDi2656542.
KEGGireh:PHG423.
PATRICi35229358. VBIRalEut6770_0351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64172 Genomic DNA. Translation: AAA98231.1.
AY305378 Genomic DNA. Translation: AAP86172.1.
RefSeqiWP_011154335.1. NC_005241.1.

3D structure databases

ProteinModelPortaliQ04539.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP86172; AAP86172; PHG423.
GeneIDi2656542.
KEGGireh:PHG423.
PATRICi35229358. VBIRalEut6770_0351.

Phylogenomic databases

eggNOGiCOG0036.
HOGENOMiHOG000259347.
KOiK01783.
OMAiHATEPNT.
OrthoDBiEOG67HK17.

Enzyme and pathway databases

UniPathwayiUPA00116.
BioCyciCNEC381666:GJUJ-6697-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR026019. Ribul_P_3_epim.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
PIRSFiPIRSF001461. RPE. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Calvin cycle enzyme pentose-5-phosphate 3-epimerase is encoded within the cfx operons of the chemoautotroph Alcaligenes eutrophus."
    Kusian B., Yoo J.-G., Bednarski R., Bowien B.
    J. Bacteriol. 174:7337-7344(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis."
    Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G.
    J. Mol. Biol. 332:369-383(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Entry informationi

Entry nameiRPEP_CUPNH
AccessioniPrimary (citable) accession number: Q04539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 22, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.