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Protein

Putative cystathionine gamma-synthase YML082W

Gene

YML082W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Catalytic activityi

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative cystathionine gamma-synthase YML082W (YML082W), Cystathionine gamma-synthase (STR2), Putative cystathionine gamma-synthase YLL058W (YLL058W)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • carbon-sulfur lyase activity Source: SGD
  • cystathionine gamma-synthase activity Source: UniProtKB-EC
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • methionine biosynthetic process Source: UniProtKB-KW
  • sulfur compound metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00051; UER00077.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative cystathionine gamma-synthase YML082W (EC:2.5.1.48)
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Gene namesi
Ordered Locus Names:YML082W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML082W.
SGDiS000004547. YML082W.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 649649Putative cystathionine gamma-synthase YML082WPRO_0000114783Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei287 – 2871PhosphoserineCombined sources
Modified residuei451 – 4511N6-(pyridoxal phosphate)lysineSequence analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04533.

PTM databases

iPTMnetiQ04533.

Interactioni

Protein-protein interaction databases

BioGridi35058. 27 interactions.
DIPiDIP-5313N.
IntActiQ04533. 3 interactions.
MINTiMINT-494142.

Structurei

3D structure databases

ProteinModelPortaliQ04533.
SMRiQ04533. Positions 306-645.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016853.
HOGENOMiHOG000193384.
InParanoidiQ04533.
KOiK01739.
OMAiKENETCF.
OrthoDBiEOG7GFBDM.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 2 hits.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04533-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSAQVATEL GQPIPLDTQH AVSVCFPTWK SVISYVEKDP KVLGCLKSGY
60 70 80 90 100
PRFWIHPSIQ KLRDILIEKY AKENETCFCF PSYRVAKRCR EYVRRKCAHR
110 120 130 140 150
NGKVRILQLA TAKPINEEQK TWKRECKIAV VFVDGAYENI LKQYWQYTGE
160 170 180 190 200
IISSRLAEYV LHELFMVEKK SSPAEEKEYI EMRYGRNLNF AFADRAKELI
210 220 230 240 250
KKRIATKVID KDEHDEEENY HFLAGNQDEQ DFQDTFLDSS LNEANHGEDH
260 270 280 290 300
DGGISGEVDS QEEPHNGLVS TIPPEPIEMS TIEEEQSVEE DAGRCALRVC
310 320 330 340 350
PERDVFLFPS GMASIFTAHR LLLQWDSLRL NRSRNGSDVT SSPPNKKTVI
360 370 380 390 400
FGFPYADTLH VLQEFNETYF LGEGDESSMK ELTKILHSGE QILAVFIETP
410 420 430 440 450
SNPLLKMGNL LELKRLSELF GFFIIIDETV GGIVNIDGLP FADIVCSSLT
460 470 480 490 500
KTFSGDSNVI GGSMVLNPQS RVYEFASRFM QLEDEYEDLL WCEDAIYLER
510 520 530 540 550
NSRDFIARTI RINYSTEYLL DKILKPHVGE NKLFKKIYYP NLTSKETLTN
560 570 580 590 600
YDMVRCKKEG GYGGLFSLTF HDEDHAAAFY DNLKLNKGPS LGTNFTLAFP
610 620 630 640
YTLMTYYHEL DMAEKFGVER NLLRISVGLE SQSILGKIFQ EAIDKTVEI
Length:649
Mass (Da):74,313
Last modified:November 1, 1997 - v1
Checksum:iFA952FBA0500BF6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46660 Genomic DNA. Translation: CAA86656.1.
BK006946 Genomic DNA. Translation: DAA09814.1.
PIRiS49644.
RefSeqiNP_013628.1. NM_001182441.1.

Genome annotation databases

EnsemblFungiiYML082W; YML082W; YML082W.
GeneIDi854892.
KEGGisce:YML082W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46660 Genomic DNA. Translation: CAA86656.1.
BK006946 Genomic DNA. Translation: DAA09814.1.
PIRiS49644.
RefSeqiNP_013628.1. NM_001182441.1.

3D structure databases

ProteinModelPortaliQ04533.
SMRiQ04533. Positions 306-645.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35058. 27 interactions.
DIPiDIP-5313N.
IntActiQ04533. 3 interactions.
MINTiMINT-494142.

PTM databases

iPTMnetiQ04533.

Proteomic databases

MaxQBiQ04533.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML082W; YML082W; YML082W.
GeneIDi854892.
KEGGisce:YML082W.

Organism-specific databases

EuPathDBiFungiDB:YML082W.
SGDiS000004547. YML082W.

Phylogenomic databases

GeneTreeiENSGT00390000016853.
HOGENOMiHOG000193384.
InParanoidiQ04533.
KOiK01739.
OMAiKENETCF.
OrthoDBiEOG7GFBDM.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00077.

Miscellaneous databases

PROiQ04533.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 2 hits.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  4. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMETX_YEAST
AccessioniPrimary (citable) accession number: Q04533
Secondary accession number(s): D6W0K0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2810 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.