Q04533 (METX_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative cystathionine gamma-synthase YML082W
EC=2.5.1.48

Alternative name(s):
O-succinylhomoserine (thiol)-lyase

Gene names

Ordered Locus Names:

YML082W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.
Miscellaneous	Present with 2810 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the trans-sulfuration enzymes family. MET7 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	cystathionine gamma-synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	Putative cystathionine gamma-synthase YML082W		PRO_0000114783
Amino acid modifications
	Modified residue	287	1	Phosphoserine Ref.4
	Modified residue	451	1	N6-(pyridoxal phosphate)lysine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q04533 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: FA952FBA0500BF6E	FASTA	649	74,313
	10 20 30 40 50 60 MVSAQVATEL GQPIPLDTQH AVSVCFPTWK SVISYVEKDP KVLGCLKSGY PRFWIHPSIQ 70 80 90 100 110 120 KLRDILIEKY AKENETCFCF PSYRVAKRCR EYVRRKCAHR NGKVRILQLA TAKPINEEQK 130 140 150 160 170 180 TWKRECKIAV VFVDGAYENI LKQYWQYTGE IISSRLAEYV LHELFMVEKK SSPAEEKEYI 190 200 210 220 230 240 EMRYGRNLNF AFADRAKELI KKRIATKVID KDEHDEEENY HFLAGNQDEQ DFQDTFLDSS 250 260 270 280 290 300 LNEANHGEDH DGGISGEVDS QEEPHNGLVS TIPPEPIEMS TIEEEQSVEE DAGRCALRVC 310 320 330 340 350 360 PERDVFLFPS GMASIFTAHR LLLQWDSLRL NRSRNGSDVT SSPPNKKTVI FGFPYADTLH 370 380 390 400 410 420 VLQEFNETYF LGEGDESSMK ELTKILHSGE QILAVFIETP SNPLLKMGNL LELKRLSELF 430 440 450 460 470 480 GFFIIIDETV GGIVNIDGLP FADIVCSSLT KTFSGDSNVI GGSMVLNPQS RVYEFASRFM 490 500 510 520 530 540 QLEDEYEDLL WCEDAIYLER NSRDFIARTI RINYSTEYLL DKILKPHVGE NKLFKKIYYP 550 560 570 580 590 600 NLTSKETLTN YDMVRCKKEG GYGGLFSLTF HDEDHAAAFY DNLKLNKGPS LGTNFTLAFP 610 620 630 640 YTLMTYYHEL DMAEKFGVER NLLRISVGLE SQSILGKIFQ EAIDKTVEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46660 Genomic DNA. Translation: CAA86656.1. BK006946 Genomic DNA. Translation: DAA09814.1.
PIR	S49644.
RefSeq	NP_013628.1. NM_001182441.1.
3D structure databases
ProteinModelPortal	Q04533.
SMR	Q04533. Positions 278-647.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5313N.
IntAct	Q04533. 3 interactions.
MINT	MINT-494142.
STRING	Q04533.
Proteomic databases
PeptideAtlas	Q04533.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML082W; YML082W; YML082W.
GeneID	854892.
KEGG	sce:YML082W.
NMPDR	fig|4932.3.peg.4665.
Organism-specific databases
CYGD	YML082w.
SGD	S000004547. YML082W.
Phylogenomic databases
eggNOG	fuNOG05525.
GeneTree	EFGT00050000003427.
HOGENOM	HBG634482.
OrthoDB	EOG4C8C1T.
Gene expression databases
ArrayExpress	Q04533.
Genevestigator	Q04533.
GermOnline	YML082W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K01739.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...
Other
NextBio	977852.

Entry information

Entry name

METX_YEAST

Accession

Primary (citable) accession number: Q04533
Secondary accession number(s): D6W0K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program