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Protein

Putative cystathionine gamma-synthase YML082W

Gene

YML082W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Miscellaneous

Present with 2810 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative cystathionine gamma-synthase YML082W (YML082W), Cystathionine gamma-synthase (STR2), Putative cystathionine gamma-synthase YLL058W (YLL058W)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • carbon-sulfur lyase activity Source: SGD
  • cystathionine gamma-synthase activity Source: GO_Central
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • methionine biosynthetic process Source: UniProtKB-KW
  • sulfur compound metabolic process Source: SGD
  • transsulfuration Source: GO_Central

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YML082W-MONOMER
UniPathwayiUPA00051; UER00077

Names & Taxonomyi

Protein namesi
Recommended name:
Putative cystathionine gamma-synthase YML082W (EC:2.5.1.48)
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Gene namesi
Ordered Locus Names:YML082W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML082W
SGDiS000004547 YML082W

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001147831 – 649Putative cystathionine gamma-synthase YML082WAdd BLAST649

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei287PhosphoserineCombined sources1
Modified residuei451N6-(pyridoxal phosphate)lysineSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04533
PaxDbiQ04533
PRIDEiQ04533

PTM databases

iPTMnetiQ04533

Interactioni

Protein-protein interaction databases

BioGridi35058, 41 interactors
DIPiDIP-5313N
IntActiQ04533, 3 interactors
STRINGi4932.YML082W

Structurei

3D structure databases

ProteinModelPortaliQ04533
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016853
HOGENOMiHOG000193384
InParanoidiQ04533
KOiK01739
OMAiFGFPYGD
OrthoDBiEOG092C18EA

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR000277 Cys/Met-Metab_PyrdxlP-dep_enz
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF01053 Cys_Met_Meta_PP, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit

Sequencei

Sequence statusi: Complete.

Q04533-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSAQVATEL GQPIPLDTQH AVSVCFPTWK SVISYVEKDP KVLGCLKSGY
60 70 80 90 100
PRFWIHPSIQ KLRDILIEKY AKENETCFCF PSYRVAKRCR EYVRRKCAHR
110 120 130 140 150
NGKVRILQLA TAKPINEEQK TWKRECKIAV VFVDGAYENI LKQYWQYTGE
160 170 180 190 200
IISSRLAEYV LHELFMVEKK SSPAEEKEYI EMRYGRNLNF AFADRAKELI
210 220 230 240 250
KKRIATKVID KDEHDEEENY HFLAGNQDEQ DFQDTFLDSS LNEANHGEDH
260 270 280 290 300
DGGISGEVDS QEEPHNGLVS TIPPEPIEMS TIEEEQSVEE DAGRCALRVC
310 320 330 340 350
PERDVFLFPS GMASIFTAHR LLLQWDSLRL NRSRNGSDVT SSPPNKKTVI
360 370 380 390 400
FGFPYADTLH VLQEFNETYF LGEGDESSMK ELTKILHSGE QILAVFIETP
410 420 430 440 450
SNPLLKMGNL LELKRLSELF GFFIIIDETV GGIVNIDGLP FADIVCSSLT
460 470 480 490 500
KTFSGDSNVI GGSMVLNPQS RVYEFASRFM QLEDEYEDLL WCEDAIYLER
510 520 530 540 550
NSRDFIARTI RINYSTEYLL DKILKPHVGE NKLFKKIYYP NLTSKETLTN
560 570 580 590 600
YDMVRCKKEG GYGGLFSLTF HDEDHAAAFY DNLKLNKGPS LGTNFTLAFP
610 620 630 640
YTLMTYYHEL DMAEKFGVER NLLRISVGLE SQSILGKIFQ EAIDKTVEI
Length:649
Mass (Da):74,313
Last modified:November 1, 1997 - v1
Checksum:iFA952FBA0500BF6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46660 Genomic DNA Translation: CAA86656.1
BK006946 Genomic DNA Translation: DAA09814.1
PIRiS49644
RefSeqiNP_013628.1, NM_001182441.1

Genome annotation databases

EnsemblFungiiYML082W; YML082W; YML082W
GeneIDi854892
KEGGisce:YML082W

Similar proteinsi

Entry informationi

Entry nameiMETX_YEAST
AccessioniPrimary (citable) accession number: Q04533
Secondary accession number(s): D6W0K0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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