Reviewed, UniProtKB/Swiss-Prot Q04524 (ILVB_KLETE)

Names and origin

Protein names

Recommended name:
    Acetolactate synthase, catabolic
      Short name=ALS
    EC=2.2.1.6

Gene names

Name:

budB

Organism

Klebsiella terrigena (Raoultella terrigena)

Taxonomic identifier

577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Raoultella

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Pathway	Polyol metabolism; 2,3-butanediol biosynthesis; (R,R)-2,3-butanediol from pyruvate: step 1/3.
Subunit structure	Homodimer.
Miscellaneous	Does not seem to require thiamine pyrophosphate By similarity.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Gene Ontology (GO)
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acetolactate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Acetolactate synthase, catabolic		PRO_0000090798
Regions
	Nucleotide binding	263 – 284	22	FAD By similarity
	Nucleotide binding	304 – 323	20	FAD By similarity
Sites
	Metal binding	447	1	Magnesium By similarity
	Binding site	159	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04524-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: C463407DF98B6F3F	FASTA	559	60,153
	10 20 30 40 50 60 MDKPRHERQW AHGADLIVSQ LEAQGVRQVF GIPGAKIDKV FDSLLDSSIR IIPVRHEANA 70 80 90 100 110 120 AFMAAAVGRI TGKAGVALVT SGPGCSNLIT GMATANSEGD PVVALGGAVK RADKAKLVHQ 130 140 150 160 170 180 SMDTVAMFSP VTKYAVEVTA SDALAEVVSN AFRAAEQGRP GSAFVSLPQD IVDGPASGST 190 200 210 220 230 240 LPASRAPQMG AAPDGAVDSV AQAIAAAKNP IFLLGLMASQ PENSRALHRH AGKKPYSGHQ 250 260 270 280 290 300 HLSGAGAVNQ DNFARFAGRV GLFNNQAGDR LLRQADLIIC IGYSPVEYEP AMWNSGTATL 310 320 330 340 350 360 VHIDVLPAYE ERNYVPDIEL VGDIAATLEK LAQRIEHRLV LTPQAADILA DRQRQRELLD 370 380 390 400 410 420 RRGAQLNQFA LHPLRIVRAM QDIVNSDVTL TVDMGSFHIW IARYLYSFRA RQVMISNGQQ 430 440 450 460 470 480 TMGVALPWAI GAWLVNPQRK VVSVSGDGGF LQSSMELETA VRLHANILHI IWVDNGYNMV 490 500 510 520 530 540 AIQEQKKYQR LSGVEFGPVD FKVYAEAFGA CGFAVESAEA LEPTLRAAMD VDGPAVVAIP 550 VDYRDNPLLM GQLHLSQIL

References

[1]

"Characterization of the genes of the 2,3-butanediol operons from Klebsiella terrigena and Enterobacter aerogenes." Blomqvist K., Nikkola M., Lehtovaara P., Suihko M.-L., Airaksinen U., Straby K.B., Knowles J.K.C., Penttilae M.E. J. Bacteriol. 175:1392-1404(1993) [PubMed: 8444801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: VTT-E-74023.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L04507 Genomic DNA. Translation: AAA25055.1.
PIR	D47069.
3D structure databases
HSSP	HSSP built from PDB template 1JSC based on UniProtKB P07342.
SMR	Q04524. Positions 8-558.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.2.1.6. 3066.
Family and domain databases
InterPro	IPR012782. Acetolactate_synth_catblc. IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02418. acolac_catab. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVB_KLETE

Accession

Primary (citable) accession number: Q04524

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)