Q04520 (BUDC_RAOTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacetyl reductase [(S)-acetoin forming]
EC=1.1.1.304

Alternative name(s):
Acetoin(diacetyl) reductase
Short name=AR
Meso-2,3-butanediol dehydrogenase

Gene names

Name:

budC

Organism

Raoultella terrigena (Klebsiella terrigena)

Taxonomic identifier

577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Raoultella

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH.
Catalytic activity	(S)-acetoin + NAD+ = diacetyl + NADH.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	acetoin catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	diacetyl reductase ((S)-acetoin forming) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Diacetyl reductase [(S)-acetoin forming]		PRO_0000054538
Regions
	Nucleotide binding	6 – 30	25	NAD By similarity
Sites
	Active site	152	1	Proton acceptor By similarity
	Active site	156	1	By similarity
	Binding site	139	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04520 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: D85801C56931BF04	FASTA	241	25,313
	10 20 30 40 50 60 MQKVALVTGA GQGIGKAIAL RLVKDGFAVA IADYNDATAT AVAAEINQAG GRAVAIKVDV 70 80 90 100 110 120 SRRDQVFAAV EQARKALGGF NVIVNNAGIA PSTPIESITE EIVDRVYNIN VKGVIWGMQA 130 140 150 160 170 180 AVEAFKKEGH GGKIVNACSQ AGHVGNPELA VYSSSKFAVR GLTQTAARDL APLGITVNGF 190 200 210 220 230 240 CPGIVKTPMW AEIDRQCRKR RANRWATARL NLPNASPLAA CRSLKTSPPA CRSSPARIPT I

References

[1]	"Characterization of the genes of the 2,3-butanediol operons from Klebsiella terrigena and Enterobacter aerogenes." Blomqvist K., Nikkola M., Lehtovaara P., Suihko M.-L., Airaksinen U., Straby K.B., Knowles J.K.C., Penttilae M.E. J. Bacteriol. 175:1392-1404(1993) [PubMed: 8444801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: VTT-E-74023.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L04507 Genomic DNA. Translation: AAA25056.1.
PIR	E47069.
3D structure databases
ProteinModelPortal	Q04520.
SMR	Q04520. Positions 1-228.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014007. 23BDH. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR02415. 23BDH. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUDC_RAOTE

Accession

Primary (citable) accession number: Q04520

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program