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Q04519

- ASM_MOUSE

UniProt

Q04519 - ASM_MOUSE

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Protein

Sphingomyelin phosphodiesterase

Gene

Smpd1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.

Catalytic activityi

Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.

GO - Molecular functioni

  1. hydrolase activity, acting on glycosyl bonds Source: UniProtKB-KW
  2. sphingomyelin phosphodiesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. ceramide biosynthetic process Source: Ensembl
  2. negative regulation of MAP kinase activity Source: Ensembl
  3. positive regulation of apoptotic process Source: Ensembl
  4. positive regulation of protein dephosphorylation Source: Ensembl
  5. response to cocaine Source: Ensembl
  6. response to drug Source: Ensembl
  7. sphingomyelin catabolic process Source: InterPro
  8. termination of signal transduction Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiREACT_199008. Glycosphingolipid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingomyelin phosphodiesterase (EC:3.1.4.12)
Alternative name(s):
Acid sphingomyelinase
Short name:
aSMase
Gene namesi
Name:Smpd1
Synonyms:Asm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:98325. Smpd1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: Ensembl
  2. extracellular vesicular exosome Source: Ensembl
  3. lamellar body Source: Ensembl
  4. lysosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4444Sequence AnalysisAdd
BLAST
Chaini45 – 627583Sphingomyelin phosphodiesterasePRO_0000002324Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi84 – 841N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi87 ↔ 163PROSITE-ProRule annotation
Disulfide bondi90 ↔ 155PROSITE-ProRule annotation
Disulfide bondi118 ↔ 129PROSITE-ProRule annotation
Glycosylationi173 – 1731N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi219 ↔ 224PROSITE-ProRule annotation
Disulfide bondi225 ↔ 248PROSITE-ProRule annotation
Glycosylationi333 – 3331N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi383 ↔ 429PROSITE-ProRule annotation
Glycosylationi393 – 3931N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi518 – 5181N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi582 ↔ 586PROSITE-ProRule annotation
Disulfide bondi592 ↔ 605PROSITE-ProRule annotation
Glycosylationi611 – 6111N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ04519.
PaxDbiQ04519.
PRIDEiQ04519.

PTM databases

PhosphoSiteiQ04519.

Expressioni

Gene expression databases

BgeeiQ04519.
CleanExiMM_SMPD1.
GenevestigatoriQ04519.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

MINTiMINT-1604473.

Structurei

3D structure databases

ProteinModelPortaliQ04519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini83 – 16785Saposin B-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the acid sphingomyelinase family.Curated
Contains 1 saposin B-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG303902.
GeneTreeiENSGT00530000063095.
HOGENOMiHOG000008599.
HOVERGENiHBG004288.
InParanoidiQ04519.
KOiK12350.
OMAiADPLCCR.
OrthoDBiEOG79PJP3.
PhylomeDBiQ04519.
TreeFamiTF313674.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR011001. Saposin-like.
IPR008139. SaposinB.
IPR011160. Sphingomy_PDE.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000948. Sphingomy_PDE. 1 hit.
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04519 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPHHRASSGQ DHLRAGWEQR LERSLPAPRV GLLWMGLGLA LVLALFDSTV
60 70 80 90 100
LWVPARAYPL PSEGHSVKFS AIAPPLQSAF GWQNLTCPAC KVLFTALNHG
110 120 130 140 150
LKKEPNVARV GSVAIKICKM LNIAPLDVCQ SAVHLFEDDV VEVWTRSVLS
160 170 180 190 200
PSEACGLLLG SSCGHWDIFS TWNISLPSVP KPPPKPPSPP APGAPVSRVL
210 220 230 240 250
FLTDLHWDHE YLEGTDPYCA DPLCCRRGSG WPPNSQKGAG FWGEYSKCDL
260 270 280 290 300
PLRTLESLLK GLGPAGPFEM VYWTGDIPAH DVWQQSRQDQ LRALTTITDL
310 320 330 340 350
VRKFLGPVPV YPAVGNHEST PVNGFPPPFI KGNQSSQWLY EAMAKAWEPW
360 370 380 390 400
LPADALHTLR IGGFYALTPR PGLRLISLNM NFCSRENFWL LINSTDPAGQ
410 420 430 440 450
LQWLVEELQA AENRGDKVHI IGHIPPGHCL KSWSWNYYKI IARYENTLAG
460 470 480 490 500
QFFGHTHVDE FEIFYDEETL SRPLAVAFLA PSATTFINLN PGYRVYQIDG
510 520 530 540 550
NYPGSSHVVL DHETYILNLT QANAAGGTPS WKRLYRARET YGLPDAMPAS
560 570 580 590 600
WHNLVYRMRD DEQLFQTFWF LYHKGHPPSE PCGTPCRLAT LCAQLSARAD
610 620
SPALCRHLMP NGSLPDANRL WSRPLLC
Length:627
Mass (Da):69,927
Last modified:February 1, 1996 - v2
Checksum:i0FFC7EA74EE71E91
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481S → T in AAH11304. (PubMed:15489334)Curated
Sequence conflicti450 – 4501G → S in AAH11304. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z14252 mRNA. Translation: CAA78619.1.
Z14132 Genomic DNA. Translation: CAA78506.1.
AK088147 mRNA. Translation: BAC40171.1.
AK145534 mRNA. Translation: BAE26489.1.
AK145702 mRNA. Translation: BAE26598.1.
AK164167 mRNA. Translation: BAE37659.1.
BC011304 mRNA. Translation: AAH11304.1.
CCDSiCCDS21653.1.
PIRiA58720. S27393.
RefSeqiNP_035551.1. NM_011421.2.
UniGeneiMm.4628.

Genome annotation databases

EnsembliENSMUST00000046983; ENSMUSP00000042187; ENSMUSG00000037049.
GeneIDi20597.
KEGGimmu:20597.
UCSCiuc009iyf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z14252 mRNA. Translation: CAA78619.1 .
Z14132 Genomic DNA. Translation: CAA78506.1 .
AK088147 mRNA. Translation: BAC40171.1 .
AK145534 mRNA. Translation: BAE26489.1 .
AK145702 mRNA. Translation: BAE26598.1 .
AK164167 mRNA. Translation: BAE37659.1 .
BC011304 mRNA. Translation: AAH11304.1 .
CCDSi CCDS21653.1.
PIRi A58720. S27393.
RefSeqi NP_035551.1. NM_011421.2.
UniGenei Mm.4628.

3D structure databases

ProteinModelPortali Q04519.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1604473.

PTM databases

PhosphoSitei Q04519.

Proteomic databases

MaxQBi Q04519.
PaxDbi Q04519.
PRIDEi Q04519.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000046983 ; ENSMUSP00000042187 ; ENSMUSG00000037049 .
GeneIDi 20597.
KEGGi mmu:20597.
UCSCi uc009iyf.2. mouse.

Organism-specific databases

CTDi 6609.
MGIi MGI:98325. Smpd1.

Phylogenomic databases

eggNOGi NOG303902.
GeneTreei ENSGT00530000063095.
HOGENOMi HOG000008599.
HOVERGENi HBG004288.
InParanoidi Q04519.
KOi K12350.
OMAi ADPLCCR.
OrthoDBi EOG79PJP3.
PhylomeDBi Q04519.
TreeFami TF313674.

Enzyme and pathway databases

Reactomei REACT_199008. Glycosphingolipid metabolism.

Miscellaneous databases

ChiTaRSi SMPD1. mouse.
NextBioi 298915.
PROi Q04519.
SOURCEi Search...

Gene expression databases

Bgeei Q04519.
CleanExi MM_SMPD1.
Genevestigatori Q04519.

Family and domain databases

Gene3Di 1.10.225.10. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR011001. Saposin-like.
IPR008139. SaposinB.
IPR011160. Sphingomy_PDE.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PIRSFi PIRSF000948. Sphingomy_PDE. 1 hit.
SMARTi SM00741. SapB. 1 hit.
[Graphical view ]
SUPFAMi SSF47862. SSF47862. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEi PS50015. SAP_B. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the acid sphingomyelinase of the mouse and the organization and complete nucleotide sequence of the gene."
    Newrzella D., Stoffel W.
    Biol. Chem. Hoppe-Seyler 373:1233-1238(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  2. Hofmann K.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 224-225 AND 384.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Hippocampus and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiASM_MOUSE
AccessioniPrimary (citable) accession number: Q04519
Secondary accession number(s): Q3UL52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two types of sphingomyelinases: ASM (acid), and NSM (neutral).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3