Q04516 (AIM33_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized oxidoreductase AIM33
EC=1.-.-.-

Alternative name(s):
Altered inheritance of mitochondria protein 33

Gene names

Name:	AIM33
Ordered Locus Names:	YML087C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	FAD By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Disruption phenotype	Increases frequency of mitochondrial genome loss. Ref.4
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Uncharacterized oxidoreductase AIM33		PRO_0000167629
Regions
	Topological domain	1 – 14	14	Extracellular Potential
	Transmembrane	15 – 35	21	Helical; Potential
	Topological domain	36 – 41	6	Cytoplasmic Potential
	Transmembrane	42 – 62	21	Helical; Potential
	Topological domain	63 – 179	117	Extracellular Potential
	Transmembrane	180 – 200	21	Helical; Potential
	Topological domain	201 – 312	112	Cytoplasmic Potential
	Domain	70 – 173	104	FAD-binding FR-type

Sequences

	Sequence		Length	Mass (Da)
	Q04516 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: F64DEA3033124EE4	FASTA	312	35,764
	10 20 30 40 50 60 MSIVETCISF VSTNPFYPFC TGLLLNCVVT PLYFWKTQNG RIVVVSLLQF VVLYATAFIS 70 80 90 100 110 120 IGTDKSLYRN KWVALPLSKK TRISRNTSLY CFKLKYPFER LHIPMGYHLA VRVTINGERL 130 140 150 160 170 180 VRYYTPVNVP NTEGHLELVV KTYKHGVVSK YFDKLKIRQY VEFKGPLGEL EYDQDTATEL 190 200 210 220 230 240 GIIAGGSGIT PVLQVLQEII PSPEDLTHIS LIYANETEDD ILMKSQLDHM AKEYPHFKVH 250 260 270 280 290 300 YVIHKPNGKW NGDVGYVTLE EMKRYLPKQA EDHRLLICGP PKMNEMVLNY AKELGWSNGF 310 HKGNGTDKVF VF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[4]	"Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis." Hess D.C., Myers C.L., Huttenhower C., Hibbs M.A., Hayes A.P., Paw J., Clore J.J., Mendoza R.M., Luis B.S., Nislow C., Giaever G., Costanzo M., Troyanskaya O.G., Caudy A.A. PLoS Genet. 5:E1000407-E1000407(2009) [PubMed: 19300474] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46660 Genomic DNA. Translation: CAA86651.1. BK006946 Genomic DNA. Translation: DAA09810.1.
PIR	S49640.
RefSeq	NP_013623.1. NM_001182446.1.
3D structure databases
ProteinModelPortal	Q04516.
SMR	Q04516. Positions 63-312.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML087C; YML087C; YML087C.
GeneID	854887.
KEGG	sce:YML087C.
NMPDR	fig|4932.3.peg.4660.
Organism-specific databases
CYGD	YML087c.
SGD	S000004552. YML087C.
Phylogenomic databases
eggNOG	fuNOG07852.
GeneTree	EFGT00050000001026.
HOGENOM	HBG591994.
OMA	YANETED.
OrthoDB	EOG4BCHX2.
Gene expression databases
ArrayExpress	Q04516.
Genevestigator	Q04516.
GermOnline	YML087C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977840.

Entry information

Entry name

AIM33_YEAST

Accession

Primary (citable) accession number: Q04516
Secondary accession number(s): D6W0J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program