Reviewed, UniProtKB/Swiss-Prot Q04515 (DYR10_ECOLX)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase type A10
    EC=1.5.1.3
Alternative name(s):
    Dihydrofolate reductase type X
      Short name=DHFRX

Gene names

Name:	dfrA10
Synonyms:	dfr10

Encoded on

Plasmid pDGO100

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Confers trimethoprim resistance.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Subunit structure	Homodimer By similarity.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	Antibiotic resistance Methotrexate resistance One-carbon metabolism Trimethoprim resistance
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to methotrexate Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Dihydrofolate reductase type A10		PRO_0000186428
Regions
	Domain	2 – 174	173	DHFR

Sequences

	Sequence		Length	Mass (Da)
	Q04515-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D3E8D81B7AC6E571	FASTA	187	21,220
	10 20 30 40 50 60 MNISLIFANE LITRAFGNQG KLPWQFIKED MQFFQKTTEN SVVVMGLNTW RSLPKMKKLG 70 80 90 100 110 120 RDFIVISSTI TEHEVLNNNI QIFKSFESFL EAFRDTTKPI NVIGGVGLLS EAIEHASTVY 130 140 150 160 170 180 MSSIHMVKPV HADVYVPVEL MNKLYSDFKY PENILWVGDP IDSVYSLSID KFVRPASLVG VPNDINT

References

[1]	"A new trimethoprim resistance gene, dhfrX, in the In7 integron of plasmid pDGO100." Parsons Y., Hall R.M., Stokes H.W. Antimicrob. Agents Chemother. 35:2436-2439(1991) [PubMed: 1804022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: VA292.
[2]	"The partial 3'-conserved segment duplications in the integrons In6 from pSa and In7 from pDGO100 have a common origin." Stokes H.W., Tomaras C., Parsons Y., Hall R.M. Plasmid 30:39-50(1993) [PubMed: 8378445] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"In34, a complex In5 family class 1 integron containing orf513 and dfrA10." Partridge S.R., Hall R.M. Antimicrob. Agents Chemother. 47:342-349(2003) [PubMed: 12499211] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L06418 Genomic DNA. Translation: AAA92749.1.
PIR	A49790.
3D structure databases
HSSP	HSSP built from PDB template 1DYR based on UniProtKB P16184.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.5.1.3. 246.
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. IPR017925. Dihydrofolate_reductase_CS. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR10_ECOLX

Accession

Primary (citable) accession number: Q04515

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)