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Q04515

- DYR10_ECOLX

UniProt

Q04515 - DYR10_ECOLX

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Protein

Dihydrofolate reductase type A10

Gene

dfrA10

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. response to antibiotic Source: UniProtKB-KW
  5. response to methotrexate Source: UniProtKB-KW
  6. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, Methotrexate resistance, One-carbon metabolism, Trimethoprim resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase type A10 (EC:1.5.1.3)
Alternative name(s):
Dihydrofolate reductase type X
Short name:
DHFRX
Gene namesi
Name:dfrA10
Synonyms:dfr10
Encoded oniPlasmid pDGO1000 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 187187Dihydrofolate reductase type A10PRO_0000186428Add
BLAST

Proteomic databases

PRIDEiQ04515.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ04515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 174173DHFRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04515-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNISLIFANE LITRAFGNQG KLPWQFIKED MQFFQKTTEN SVVVMGLNTW
60 70 80 90 100
RSLPKMKKLG RDFIVISSTI TEHEVLNNNI QIFKSFESFL EAFRDTTKPI
110 120 130 140 150
NVIGGVGLLS EAIEHASTVY MSSIHMVKPV HADVYVPVEL MNKLYSDFKY
160 170 180
PENILWVGDP IDSVYSLSID KFVRPASLVG VPNDINT
Length:187
Mass (Da):21,220
Last modified:November 1, 1996 - v1
Checksum:iD3E8D81B7AC6E571
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06418 Genomic DNA. Translation: AAA92749.1.
PIRiA49790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06418 Genomic DNA. Translation: AAA92749.1 .
PIRi A49790.

3D structure databases

ProteinModelPortali Q04515.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q04515.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A new trimethoprim resistance gene, dhfrX, in the In7 integron of plasmid pDGO100."
    Parsons Y., Hall R.M., Stokes H.W.
    Antimicrob. Agents Chemother. 35:2436-2439(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: VA292.
  2. "The partial 3'-conserved segment duplications in the integrons In6 from pSa and In7 from pDGO100 have a common origin."
    Stokes H.W., Tomaras C., Parsons Y., Hall R.M.
    Plasmid 30:39-50(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "In34, a complex In5 family class 1 integron containing orf513 and dfrA10."
    Partridge S.R., Hall R.M.
    Antimicrob. Agents Chemother. 47:342-349(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDYR10_ECOLX
AccessioniPrimary (citable) accession number: Q04515
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Confers trimethoprim resistance.

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3