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Protein

5-aminolevulinate synthase 1

Gene

hemA

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathway:iprotoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase 1 (hemA), 5-aminolevulinate synthase 2 (hemT)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211SubstrateBy similarity
Binding sitei137 – 1371SubstrateBy similarity
Binding sitei156 – 1561SubstrateBy similarity
Binding sitei189 – 1891Pyridoxal phosphateBy similarity
Binding sitei217 – 2171Pyridoxal phosphateBy similarity
Binding sitei245 – 2451Pyridoxal phosphateBy similarity
Active sitei248 – 2481By similarity
Binding sitei277 – 2771Pyridoxal phosphateBy similarity
Binding sitei278 – 2781Pyridoxal phosphateBy similarity
Binding sitei363 – 3631SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-1613-MONOMER.
UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase 1 (EC:2.3.1.37)
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene namesi
Name:hemA
Ordered Locus Names:RHOS4_15770
ORF Names:RSP_2984
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002703 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4074075-aminolevulinate synthase 1PRO_0000163829Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei248 – 2481N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Protein-protein interaction databases

STRINGi272943.RSP_2984.

Structurei

3D structure databases

ProteinModelPortaliQ04512.
SMRiQ04512. Positions 1-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0156.
HOGENOMiHOG000221020.
KOiK00643.
OMAiKEITVWC.
OrthoDBiEOG6Q8HZD.
PhylomeDBiQ04512.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04512-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYNLALDTA LNRLHTEGRY RTFIDIERRK GAFPKAMWRK PDGSEKEITV
60 70 80 90 100
WCGNDYLGMG QHPVVLGAMH EALDSTGAGS GGTRNISGTT LYHKRLEAEL
110 120 130 140 150
ADLHGKEAAL VFSSAYIAND ATLSTLPQLI PGLVIVSDKL NHASMIEGIR
160 170 180 190 200
RSGTEKHIFK HNDLDDLRRI LTSIGKDRPI LVAFESVYSM DGDFGRIEEI
210 220 230 240 250
CDIADEFGAL KYIDEVHAVG MYGPRGGGVA ERDGLMDRID IINGTLGKAY
260 270 280 290 300
GVFGGYIAAS SKMCDAVRSY APGFIFSTSL PPVVAAGAAA SVRHLKGDVE
310 320 330 340 350
LREKHQTQAR ILKMRLKGLG LPIIDHGSHI VPVHVGDPVH CKMISDMLLE
360 370 380 390 400
HFGIYVQPIN FPTVPRGTER LRFTPSPVHD SGMIDHLVKA MDVLWQHCAL

NRAEVVA
Length:407
Mass (Da):44,616
Last modified:October 1, 1993 - v1
Checksum:iDCDE2484BFACC7F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07490 Genomic DNA. Translation: AAA72325.1.
CP000143 Genomic DNA. Translation: ABA79145.1.
RefSeqiWP_011337894.1. NZ_AKVW01000001.1.
YP_353046.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA79145; ABA79145; RSP_2984.
GeneIDi3720398.
KEGGirsp:RSP_2984.
PATRICi23153077. VBIRhoSph57909_1924.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07490 Genomic DNA. Translation: AAA72325.1.
CP000143 Genomic DNA. Translation: ABA79145.1.
RefSeqiWP_011337894.1. NZ_AKVW01000001.1.
YP_353046.1. NC_007493.2.

3D structure databases

ProteinModelPortaliQ04512.
SMRiQ04512. Positions 1-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_2984.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA79145; ABA79145; RSP_2984.
GeneIDi3720398.
KEGGirsp:RSP_2984.
PATRICi23153077. VBIRhoSph57909_1924.

Phylogenomic databases

eggNOGiCOG0156.
HOGENOMiHOG000221020.
KOiK00643.
OMAiKEITVWC.
OrthoDBiEOG6Q8HZD.
PhylomeDBiQ04512.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.
BioCyciRSPH272943:GJAS-1613-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of the Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthase isozymes."
    Neidle E.L., Kaplan S.
    J. Bacteriol. 175:2292-2303(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Entry informationi

Entry nameiHEM1_RHOS4
AccessioniPrimary (citable) accession number: Q04512
Secondary accession number(s): Q3J239
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 22, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.