Q04512 (HEM1_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-aminolevulinate synthase 1
EC=2.3.1.37

Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase

Gene names

Name:	hemA
Ordered Locus Names:	RHOS4_15770
ORF Names:	RSP_2984

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	5-aminolevulinate synthase 1		PRO_0000163829
Amino acid modifications
	Modified residue	248	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04512 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: DCDE2484BFACC7F0	FASTA	407	44,616
	10 20 30 40 50 60 MDYNLALDTA LNRLHTEGRY RTFIDIERRK GAFPKAMWRK PDGSEKEITV WCGNDYLGMG 70 80 90 100 110 120 QHPVVLGAMH EALDSTGAGS GGTRNISGTT LYHKRLEAEL ADLHGKEAAL VFSSAYIAND 130 140 150 160 170 180 ATLSTLPQLI PGLVIVSDKL NHASMIEGIR RSGTEKHIFK HNDLDDLRRI LTSIGKDRPI 190 200 210 220 230 240 LVAFESVYSM DGDFGRIEEI CDIADEFGAL KYIDEVHAVG MYGPRGGGVA ERDGLMDRID 250 260 270 280 290 300 IINGTLGKAY GVFGGYIAAS SKMCDAVRSY APGFIFSTSL PPVVAAGAAA SVRHLKGDVE 310 320 330 340 350 360 LREKHQTQAR ILKMRLKGLG LPIIDHGSHI VPVHVGDPVH CKMISDMLLE HFGIYVQPIN 370 380 390 400 FPTVPRGTER LRFTPSPVHD SGMIDHLVKA MDVLWQHCAL NRAEVVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of the Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthase isozymes." Neidle E.L., Kaplan S. J. Bacteriol. 175:2292-2303(1993) [PubMed: 8468290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07490 Genomic DNA. Translation: AAA72325.1. CP000143 Genomic DNA. Translation: ABA79145.1.
RefSeq	YP_353046.1. NC_007493.1.
3D structure databases
ProteinModelPortal	Q04512.
SMR	Q04512. Positions 1-395.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04512.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3720398.
GenomeReviews	Gene locus RHOS4_15770 in contig CP000143_GR.
KEGG	rsp:RSP_2984.
NMPDR	fig|272943.3.peg.1662.
PATRIC	23153077. VBIRhoSph57909_1924.
Phylogenomic databases
eggNOG	COG0156.
HOGENOM	HBG649839.
OMA	HLVKAMD.
PhylomeDB	Q04512.
ProtClustDB	PRK09064.
Enzyme and pathway databases
BioCyc	RSPH272943:RSP_2984-MONOMER.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00643.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_RHOS4

Accession

Primary (citable) accession number: Q04512
Secondary accession number(s): Q3J239

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program