Q04511 (UFO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin ligase complex F-box protein UFO1

Gene names

Name:	UFO1
Ordered Locus Names:	YML088W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins By similarity. Ref.3
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1. Component of the probable SCF(UFO1) complex containing CDC53, SKP1, RBX1 and UFO1. Ref.4 Ref.6
Miscellaneous	Present with 639 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 1 F-box domain. Contains 3 UIM (ubiquitin-interacting motif) repeats.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from mutant phenotype Ref.3. Source: SGD cellular response to methylmercury Inferred from mutant phenotype. Source: SGD protein ubiquitination Inferred by curator Ref.6. Source: GOC response to DNA damage stimulus Inferred from mutant phenotype Ref.3. Source: SGD
Cellular component	SCF ubiquitin ligase complex Inferred from direct assay Ref.4Ref.6. Source: SGD cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1	P52286	4	EBI-20020,EBI-4090

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 668	668	Ubiquitin ligase complex F-box protein UFO1		PRO_0000119970
Regions
	Domain	5 – 51	47	F-box
	Repeat	547 – 566	20	UIM 1
	Repeat	583 – 602	20	UIM 2
	Repeat	651 – 668	18	UIM 3
Amino acid modifications
	Modified residue	514	1	Phosphothreonine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q04511 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 10CA467CF9417FBC	FASTA	668	76,860
	10 20 30 40 50 60 MERPGLVLQD LPPEILINIF SHLDEKDLFT LQELSTHFRN LIHDEELWKN LFKSRVHTTH 70 80 90 100 110 120 FPTFSQSSKF SVEYIERTRG LHHWQHNKAI RTKYTIIPTR NWDQPSIERI VFDYPRVAAY 130 140 150 160 170 180 NDGTITILQL QNHKRQKKFK KLIYIPCTTP QGCSTMDFNI NAAVFGRFDG RVFGKLLSNK 190 200 210 220 230 240 SYLTPVMEFT GRHSAGVTAI CNSESWDTSR EDWSVSGSEN GEIIWWCENK LVKMWKVSNR 250 260 270 280 290 300 VIWKLAFFKD WTLIMDDEKL YIIHQMQELH SIDIPKDLDE QPMRVRFFKM DFGSMTLVLA 310 320 330 340 350 360 DLNNVYTISV NPNGNFGNLR KLEMPEQICA VEIDEKTSQR EQNWQFAGDD GCYISLLTTQ 370 380 390 400 410 420 NTLYIINIRD LSSSGLKVQC KISFDEQVYV SQVTNLIVVV ALPNVLQILN AMTGELIKTV 430 440 450 460 470 480 LKTEKFPEFL KVSQDKIIMG SGNVLNYLKF VSSDSKKHHH STKGKNTVSN KWNETLNTEL 490 500 510 520 530 540 QYYDEDEDLR RKRQSEISRL IDAYGGDLEL SGDTDEENDI QLRIALLESQ EAQARNQAEA 550 560 570 580 590 600 GEPVGDDEDE QLRRALEESQ LIYETQTNSS ANHGNNTNDE IDEDDEEFLR AIRQSRVEDE 610 620 630 640 650 660 RRRHLRNHTT GRRNGPLSDD NFATYGAAES SERTSTENTI GSSVGVDASN NVDEDLQLAI ALSLSEIN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Functions of the DNA damage response pathway target Ho endonuclease of yeast for degradation via the ubiquitin-26S proteasome system." Kaplun L., Ivantsiv Y., Kornitzer D., Raveh D. Proc. Natl. Acad. Sci. U.S.A. 97:10077-10082(2000) [PubMed: 10963670] [Abstract] Cited for: FUNCTION IN HO DEGRADATION.
[4]	"Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly." Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J. Nat. Cell Biol. 3:384-391(2001) [PubMed: 11283612] [Abstract] Cited for: INTERACTION WITH SKP1.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro." Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M. Proteins 54:455-467(2004) [PubMed: 14747994] [Abstract] Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(UFO1) COMPLEX.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-514, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46660 Genomic DNA. Translation: CAA86650.1. BK006946 Genomic DNA. Translation: DAA09809.1.
PIR	S49639.
RefSeq	NP_013622.1. NM_001182447.1.
3D structure databases
ProteinModelPortal	Q04511.
SMR	Q04511. Positions 6-51, 348-421.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1953N.
IntAct	Q04511. 2 interactions.
MINT	MINT-405291.
STRING	Q04511.
Proteomic databases
PeptideAtlas	Q04511.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML088W; YML088W; YML088W.
GeneID	854886.
KEGG	sce:YML088W.
NMPDR	fig|4932.3.peg.4659.
Organism-specific databases
CYGD	YML088w.
SGD	S000004553. UFO1.
Phylogenomic databases
eggNOG	fuNOG04296.
HOGENOM	HBG203530.
OMA	DEELWKN.
OrthoDB	EOG405W8Z.
Gene expression databases
ArrayExpress	Q04511.
Genevestigator	Q04511.
GermOnline	YML088W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. IPR022364. F-box_dom_Skp2-like. IPR003903. Ubiquitin-int_motif. IPR015943. WD40/YVTN_repeat-like_dom. IPR011046. WD40_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 3 hits.
KO	K15073.
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. SM00726. UIM. 4 hits. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit. SSF50978. WD40_like. 1 hit.
PROSITE	PS50181. FBOX. 1 hit. PS50330. UIM. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	977837.

Entry information

Entry name

UFO1_YEAST

Accession

Primary (citable) accession number: Q04511
Secondary accession number(s): D6W0J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program