U3 small nucleolar RNA-associated protein 14 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q04500 (UTP14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
U3 small nucleolar RNA-associated protein 14
Short name=U3 snoRNA-associated protein 14

Alternative name(s):
U three protein 14

Gene names

Name:	UTP14
Ordered Locus Names:	YML093W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	899 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA. Ref.3
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.3
Subcellular location	Nucleus › nucleolus Ref.3.
Miscellaneous	Present with 1470 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the UTP14 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 15489292. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 15489292. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 15489292. Source: SGD
Cellular_component	nucleolus Inferred from direct assay Ref.3. Source: SGD small-subunit processome Inferred from direct assay Ref.3. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 899

899

U3 small nucleolar RNA-associated protein 14

PRO_0000065742

Regions

Nucleotide binding

260 – 267

ATP Potential

Compositional bias

162 – 168

Poly-Ser

Compositional bias

179 – 182

Poly-Glu

Compositional bias

704 – 712

Poly-Lys

Amino acid modifications

Modified residue

Phosphoserine Ref.5 Ref.7 Ref.8

Modified residue

Phosphoserine Ref.5 Ref.7

Modified residue

151

Phosphoserine Ref.5 Ref.7 Ref.8

Modified residue

423

Phosphoserine Ref.5 Ref.7 Ref.8

Modified residue

424

Phosphoserine Ref.5 Ref.7

Modified residue

488

Phosphoserine Ref.7 Ref.8

Modified residue

500

Phosphoserine Ref.7 Ref.8

Modified residue

562

Phosphoserine Ref.5 Ref.7 Ref.8

Modified residue

668

Phosphoserine Ref.6 Ref.7

Modified residue

738

Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

Q04500 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 0D4FC90D1CB3CFF1
Show »

FASTA

899

103,023

        10         20         30         40         50         60 
MAKKKSKSRS KSSRRVLDAL QLAEREINGE FDNSSDNDKR HDARRNGTVV NLLKRSKGDT 

        70         80         90        100        110        120 
NSDEDDIDSE SFEDEELNSD EALGSDDDYD ILNSKFSQTI RDKKENANYQ EEEDEGGYTS 

       130        140        150        160        170        180 
IDEEDLMPLS QVWDMDEKTA QSNGNDDEDA SPQLKLQDTD ISSESSSSEE SESESEDDEE 

       190        200        210        220        230        240 
EEDPFDEISE DEEDIELNTI TSKLIDETKS KAPKRLDTYG SGEANEYVLP SANAASGASG 

       250        260        270        280        290        300 
KLSLTDMMNV IDDRQVIENA NLLKGKSSTY EVPLPQRIQQ RHDRKAAYEI SRQEVSKWND 

       310        320        330        340        350        360 
IVQQNRRADH LIFPLNKPTE HNHASAFTRT QDVPQTELQE KVDQVLQESN LANPEKDSKF 

       370        380        390        400        410        420 
EELSTAKMTP EEMRKRTTEM RLMRELMFRE ERKARRLKKI KSKTYRKIKK KELMKNRELA 

       430        440        450        460        470        480 
AVSSDEDNED HDIARAKERM TLKHKTNSKW AKDMIKHGMT NDAETREEME EMLRQGERLK 

       490        500        510        520        530        540 
AKMLDRNSDD EEDGRVQTLS DVENEEKENI DSEALKSKLG KTGVMNMAFM KNGEAREREA 

       550        560        570        580        590        600 
NKETLRQLRA VENGDDIKLF ESDEEETNGE NIQINKGRRI YTPGSLESNK DMNELNDHTR 

       610        620        630        640        650        660 
KENKVDESRS LENRLRAKNS GQSKNARTNA EGAIIVEEES DGEPLQDGQN NQQDEEAKDV 

       670        680        690        700        710        720 
NPWLANESDE EHTVKKQSSK VNVIDKDSSK NVKAMNKMEK AELKQKKKKK GKSNDDEDLL 

       730        740        750        760        770        780 
LTADDSTRLK IVDPYGGSDD EQGDNVFMFK QQDVIAEAFA GDDVVAEFQE EKKRVIDDED 

       790        800        810        820        830        840 
DKEVDTTLPG WGEWAGAGSK PKNKKRKFIK KVKGVVNKDK RRDKNLQNVI INEKVNKKNL 

       850        860        870        880        890 
KYQSSAVPFP FENREQYERS LRMPIGQEWT SRASHQELIK PRIMTKPGQV IDPLKAPFK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; SER-35; SER-151; SER-423; SER-424; SER-562 AND SER-738, MASS SPECTROMETRY. Strain: ADR376.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-668 AND SER-738, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; SER-35; SER-151; SER-423; SER-424; SER-488; SER-500; SER-562; SER-668 AND SER-738, MASS SPECTROMETRY.
[8]	"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution." Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O. Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; SER-151; SER-423; SER-488; SER-500; SER-562 AND SER-738, MASS SPECTROMETRY.
[9]	"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB." Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R. Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46660 Genomic DNA. Translation: CAA86645.1. BK006946 Genomic DNA. Translation: DAA09806.1.
PIR	S49634.
RefSeq	NP_013617.1. NM_001182452.1.
3D structure databases
ProteinModelPortal	Q04500.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	35050. 34 interactions.
DIP	DIP-6410N.
IntAct	Q04500. 3 interactions.
MINT	MINT-8285383.
STRING	4932.YML093W.
Proteomic databases
PaxDb	Q04500.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML093W; YML093W; YML093W.
GeneID	854881.
KEGG	sce:YML093W.
Organism-specific databases
CYGD	YML093w.
SGD	S000004558. UTP14.
Phylogenomic databases
eggNOG	COG5644.
GeneTree	ENSGT00390000008142.
HOGENOM	HOG000074497.
KO	K14567.
OMA	ICIEAND.
OrthoDB	EOG7HQNJB.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32678-MONOMER.
Gene expression databases
Genevestigator	Q04500.
Family and domain databases
InterPro	IPR006709. SSU_processome_Utp14. [Graphical view]
PANTHER	PTHR14150. PTHR14150. 1 hit.
Pfam	PF04615. Utp14. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977828.
PRO	Q04500.

Entry information

Entry name

UTP14_YEAST

Accession

Primary (citable) accession number: Q04500
Secondary accession number(s): D6W0J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 19, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents