Q04493 (PFD5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prefoldin subunit 5

Alternative name(s):
Genes involved in microtubule biogenesis protein 5
Gim complex subunit 5
Short name=GimC subunit 5

Gene names

Name:	GIM5
Synonyms:	PFD5
Ordered Locus Names:	YML094W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Subunit structure	Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.
Miscellaneous	Present with 2900 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the prefoldin subunit alpha family.

Ontologies

Keywords
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro tubulin complex assembly Inferred from mutant phenotype Ref.3. Source: SGD
Cellular component	prefoldin complex Inferred from electronic annotation. Source: InterPro
Molecular function	tubulin binding Inferred from direct assay Ref.3. Source: SGD unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAC10	P48363	6	EBI-13253,EBI-13239
YKE2	P52553	3	EBI-13253,EBI-13260

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 163	163	Prefoldin subunit 5		PRO_0000153668

Sequences

	Sequence		Length	Mass (Da)
	Q04493 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 24C71A33885CEE63	FASTA	163	18,356
	10 20 30 40 50 60 MSSQKIDLTK LNPEQLNAVK QQFDQELQHF TQSLQALTMA KGKFTECIDD IKTVSQAGNE 70 80 90 100 110 120 GQKLLVPASA SLYIPGKIVD NKKFMVDIGT GYYVEKSAEA AIAFYQKKVD KLNKESVQIQ 130 140 150 160 DIIKEKTQYS LSIEAQIRQA AIRQHEAMSK QQQQQQKKES STA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A novel protein complex promoting formation of functional alpha- and gamma-tubulin." Geissler S., Siegers K., Schiebel E. EMBO J. 17:952-966(1998) [PubMed: 9463374] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system." Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E., Hartl F.U. EMBO J. 18:75-84(1999) [PubMed: 9878052] [Abstract] Cited for: IDENTIFICATION IN THE PREFOLDIN COMPLEX.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46660 Genomic DNA. Translation: CAA86644.1. BK006946 Genomic DNA. Translation: DAA09805.1.
PIR	S49633.
RefSeq	NP_013616.1. NM_001182453.1.
3D structure databases
ProteinModelPortal	Q04493.
SMR	Q04493. Positions 12-147.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1332N.
IntAct	Q04493. 12 interactions.
MINT	MINT-406816.
STRING	Q04493.
Proteomic databases
PeptideAtlas	Q04493.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML094W; YML094W; YML094W.
GeneID	854879.
KEGG	sce:YML094W.
NMPDR	fig|4932.3.peg.4653.
Organism-specific databases
CYGD	YML094w.
SGD	S000004559. GIM5.
Phylogenomic databases
eggNOG	fuNOG09881.
GeneTree	EFGT00050000005734.
HOGENOM	HBG522331.
OMA	KDYFKRR.
OrthoDB	EOG4TJ03P.
Gene expression databases
ArrayExpress	Q04493.
Genevestigator	Q04493.
GermOnline	YML094W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011599. PFD_alpha. IPR009053. Prefoldin. IPR004127. Prefoldin_subunit. [Graphical view]
KO	K04797.
Pfam	PF02996. Prefoldin. 1 hit. [Graphical view]
SUPFAM	SSF46579. Prefoldin. 1 hit.
TIGRFAMs	TIGR00293. TIGR00293. 1 hit.
ProtoNet	Search...
Other
NextBio	977825.

Entry information

Entry name

PFD5_YEAST

Accession

Primary (citable) accession number: Q04493
Secondary accession number(s): D6W0J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program