Reviewed, UniProtKB/Swiss-Prot Q04493 (PFD5_YEAST)

Names and origin

Protein names

Recommended name:
    Prefoldin subunit 5
Alternative name(s):
    Genes involved in microtubule biogenesis protein 5
    Gim complex subunit 5
      Short name=GimC subunit 5

Gene names

Name:	GIM5
Synonyms:	PFD5
Ordered Locus Names:	YML094W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Subunit structure	Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.
Miscellaneous	Present with 2900 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the prefoldin subunit alpha family.

Ontologies

Keywords
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro tubulin complex assembly Ref.2 Inferred from mutant phenotype. Source: SGD
Cellular component	prefoldin complex Ref.2 Inferred from physical interaction. Source: SGD
Molecular function	tubulin binding Ref.2 Inferred from direct assay. Source: SGD unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAC10	P48363	1	EBI-13253,EBI-13239
YKE2	P52553	1	EBI-13253,EBI-13260

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 163	163	Prefoldin subunit 5		PRO_0000153668

Sequences

	Sequence		Length	Mass (Da)
	Q04493-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 24C71A33885CEE63	FASTA	163	18,356
	10 20 30 40 50 60 MSSQKIDLTK LNPEQLNAVK QQFDQELQHF TQSLQALTMA KGKFTECIDD IKTVSQAGNE 70 80 90 100 110 120 GQKLLVPASA SLYIPGKIVD NKKFMVDIGT GYYVEKSAEA AIAFYQKKVD KLNKESVQIQ 130 140 150 160 DIIKEKTQYS LSIEAQIRQA AIRQHEAMSK QQQQQQKKES STA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A novel protein complex promoting formation of functional alpha- and gamma-tubulin." Geissler S., Siegers K., Schiebel E. EMBO J. 17:952-966(1998) [PubMed: 9463374] [Abstract] Cited for: CHARACTERIZATION.
[3]	"Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system." Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E., Hartl F.U. EMBO J. 18:75-84(1999) [PubMed: 9878052] [Abstract] Cited for: IDENTIFICATION IN THE PREFOLDIN COMPLEX.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46660 Genomic DNA. Translation: CAA86644.1.
PIR	S49633.
RefSeq	NP_013616.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1332N.
IntAct	Q04493. 13 interactions.
STRING	Q04493.
Proteomic databases
PeptideAtlas	Q04493.
Genome annotation databases
Ensembl	YML094W; YML094W; YML094W; Saccharomyces cerevisiae. [Genome view]
GeneID	854879.
KEGG	sce:YML094W.
NMPDR	fig|4932.3.peg.4653.
Organism-specific databases
CYGD	YML094w.
SGD	S000004559. GIM5.
Phylogenomic databases
HOGENOM	HBG522331.
OMA	KRRTRAY.
OrthoDB	EOG9WWT1X.
Gene expression databases
ArrayExpress	Q04493.
Genevestigator	Q04493.
GermOnline	YML094W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011599. PFD_alpha. IPR004127. PFD_alpha-like. IPR009053. Prefoldin. [Graphical view]
Pfam	PF02996. Prefoldin. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977825.

Entry information

Entry name

PFD5_YEAST

Accession

Primary (citable) accession number: Q04493

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 15, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)