Reviewed, UniProtKB/Swiss-Prot Q04489 (ASND1_YEAST)

Names and origin

Protein names

Recommended name:
    Asparagine synthetase domain-containing protein YML096W

Gene names

Ordered Locus Names:

YML096W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cytoplasm.
Miscellaneous	Present with 3750 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Contains 1 asparagine synthetase domain. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: SGD
Molecular function	asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-27924,EBI-27924

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 525	524	Asparagine synthetase domain-containing protein YML096W		PRO_0000056940
Regions
	Domain	2 – 209	208	Glutamine amidotransferase type-2
	Domain	210 – 523	314	Asparagine synthetase
Sites
	Active site	2	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04489-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 862705A8C789AA6C	FASTA	525	59,469
	10 20 30 40 50 60 MCGILLHYCP NNNYLNDELI EFPEGTEFGD TTCTNESSIF NKIIPYIAAR GPNYSSLRAV 70 80 90 100 110 120 KAYRISWFSS VLSLRQPFTK QSINVDDRYF LQFNGELYNK EISQGDNDSL YIASMLQNLK 130 140 150 160 170 180 EGMGVIDVIK SLEGEYAYTI YDVNSSKLYF GRDPIGRRSL SYSVTPDNEL YVASVTGSAG 190 200 210 220 230 240 SFQDCIGGVI YEYDTRTKLL NSNQRSHLPY EVTSEIDLNF TSLSEVSKNL YAVLRDSVKK 250 260 270 280 290 300 RVESIHPRHI ENSPIAVLFS GGIDCSVIVA LICEVLQEND YKCGKPVIEL LNVSFENPRT 310 320 330 340 350 360 GLFPSDTPDR KLSINSAKTL QNLYPNVDIK LVEVDVPYDE YLKWKPFVIN LMYPKQTEMD 370 380 390 400 410 420 LSIAIAFFFA SRGRGFLTSL NGERTPYQRH GIVLFSGLGA DELYGGYHKF ANKPPHELVE 430 440 450 460 470 480 ELTRQINNIY DRNLNRDDKV IAHNGVEVRY PFLDEYVIKL STAEIPINFK VNKLILRKVA 490 500 510 520 SQYLKLDGIS SEPKRAIQFG AKSAKMTKDG NKHGTDLLKE NRNCS

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46660 Genomic DNA. Translation: CAA86641.1.
PIR	S49630.
RefSeq	NP_013613.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q04489. 1 interaction.
Proteomic databases
PeptideAtlas	Q04489.
Genome annotation databases
Ensembl	YML096W; YML096W; YML096W; Saccharomyces cerevisiae. [Genome view]
GeneID	854877.
KEGG	sce:YML096W.
NMPDR	fig|4932.3.peg.4650.
Organism-specific databases
CYGD	YML096w.
SGD	S000004562. YML096W.
Phylogenomic databases
HOGENOM	Q04489.
OMA	ECEQAPD
OrthoDB	EOG9WSXSZ
Gene expression databases
ArrayExpress	Q04489.
Genevestigator	Q04489.
GermOnline	YML096W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001962. Asn_synthase. IPR017932. GATase_II. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00733. Asn_synthase. 1 hit. [Graphical view]
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977819.

Entry information

Entry name

ASND1_YEAST

Accession

Primary (citable) accession number: Q04489

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)