Kinetochore protein SPC24 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q04477 (SPC24_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Kinetochore protein SPC24

Gene names

Name:	SPC24
Ordered Locus Names:	YMR117C
ORF Names:	YM9718.16C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity. Ref.4 Ref.5 Ref.6
Subunit structure	Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere. Can also interact with MPS2. Ref.4 Ref.8
Subcellular location	Nucleus. Chromosome › centromere › kinetochore. Note: Associated with kinetochores. Ref.3 Ref.4 Ref.5
Miscellaneous	Present with 1750 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SPC24 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Centromere Chromosome Kinetochore Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from genetic interaction Ref.4. Source: SGD microtubule nucleation Inferred from physical interaction PubMed 9153752. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Ndc80 complex Inferred from direct assay Ref.3 Ref.4. Source: SGD condensed nuclear chromosome kinetochore Inferred from direct assay PubMed 11511347. Source: SGD
Molecular_function	structural constituent of cytoskeleton Inferred from physical interaction PubMed 9585415. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
itself		3	EBI-27228,EBI-27228
CDC12	P32468	3	EBI-27228,EBI-4182
MMR1	Q06324	3	EBI-27228,EBI-37386
MPS2	P53159	8	EBI-27228,EBI-23834
NUF2	P33895	6	EBI-27228,EBI-12377
SPC25	P40014	18	EBI-27228,EBI-22458
TID3	P40460	17	EBI-27228,EBI-25247

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 213

213

Kinetochore protein SPC24

PRO_0000203294

Regions

Coiled coil

54 – 123

Potential

Amino acid modifications

Modified residue

130

Phosphothreonine Ref.9

Secondary structure

213

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q04477 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 8BE4B8619DD4C8FB
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FASTA

213

24,604

        10         20         30         40         50         60 
MSQKDNLLDN PVEFLKEVRE SFDIQQDVDA MKRIRHDLDV IKEESEARIS KEHSKVSESN 

        70         80         90        100        110        120 
KKLNAERINV AKLEGDLEYT NEESNEFGSK DELVKLLKDL DGLERNIVSL RSELDEKMKL 

       130        140        150        160        170        180 
YLKDSEIIST PNGSKIKAKV IEPELEEQSA VTPEANENIL KLKLYRSLGV ILDLENDQVL 

       190        200        210 
INRKNDGNID ILPLDNNLSD FYKTKYIWER LGK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control." Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E. EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TID3 AND NUF2.
[4]	"The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation." Wigge P.A., Kilmartin J.V. J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
[5]	"Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication." Le Masson I., Saveanu C., Chevalier A., Namane A., Gobin R., Fromont-Racine M., Jacquier A., Mann C. Mol. Microbiol. 43:1431-1443(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPS2; NDC80 AND SPC25, SUBCELLULAR LOCATION.
[6]	"The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity." McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T. Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE NDC80 COMPLEX.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association." Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U. Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-130, MASS SPECTROMETRY.
[10]	"Molecular organization of the Ndc80 complex, an essential kinetochore component." Wei R.R., Sorger P.K., Harrison S.C. Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z49702 Genomic DNA. Translation: CAA89755.1.
BK006946 Genomic DNA. Translation: DAA10014.1.

PIR

S54579.

RefSeq

NP_013835.1. NM_001182617.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FTX	X-ray	1.90	B	154-213	[»]
2FV4	NMR	-	B	138-213	[»]
4GEQ	X-ray	2.01	B/D	155-213	[»]

ProteinModelPortal

Q04477.

SMR

Q04477. Positions 155-213.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-893N.

IntAct

Q04477. 64 interactions.

MINT

MINT-404120.

STRING

4932.YMR117C.

Proteomic databases

PaxDb

Q04477.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YMR117C; YMR117C; YMR117C.

GeneID

855144.

KEGG

sce:YMR117C.

Organism-specific databases

CYGD

YMR117c.

SGD

S000004723. SPC24.

Phylogenomic databases

eggNOG

euNOG07929.

HOGENOM

HOG000000760.

K11557.

OMA

ARISKEH.

OrthoDB

EOG4K0TZ2.

Gene expression databases

Genevestigator

Q04477.

GermOnline

YMR117C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR013252. Ndc80_Spc24.
[Graphical view]

Pfam

PF08286. Spc24. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q04477.

NextBio

978540.

Entry information

Entry name

SPC24_YEAST

Accession

Primary (citable) accession number: Q04477
Secondary accession number(s): D6VZU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents