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Q04477

- SPC24_YEAST

UniProt

Q04477 - SPC24_YEAST

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Protein

Kinetochore protein SPC24

Gene

SPC24

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.3 Publications

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  1. chromosome segregation Source: SGD
  2. microtubule nucleation Source: SGD
  3. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32812-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein SPC24
Gene namesi
Name:SPC24
Ordered Locus Names:YMR117C
ORF Names:YM9718.16C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR117c.
SGDiS000004723. SPC24.

Subcellular locationi

Nucleus. Chromosomecentromerekinetochore
Note: Associated with kinetochores.

GO - Cellular componenti

  1. condensed nuclear chromosome, centromeric region Source: SGD
  2. condensed nuclear chromosome kinetochore Source: SGD
  3. Ndc80 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 213212Kinetochore protein SPC24PRO_0000203294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ04477.
PaxDbiQ04477.

Expressioni

Gene expression databases

GenevestigatoriQ04477.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere. Can also interact with MPS2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-27228,EBI-27228
CDC12P324683EBI-27228,EBI-4182
MMR1Q063243EBI-27228,EBI-37386
MPS2P531598EBI-27228,EBI-23834
NUF2P338958EBI-27228,EBI-12377
SPC25P4001420EBI-27228,EBI-22458
TID3P4046019EBI-27228,EBI-25247

Protein-protein interaction databases

BioGridi35293. 186 interactions.
DIPiDIP-893N.
IntActiQ04477. 62 interactions.
MINTiMINT-404120.
STRINGi4932.YMR117C.

Structurei

Secondary structure

1
213
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi157 – 16711Combined sources
Beta strandi170 – 1734Combined sources
Helixi174 – 1763Combined sources
Beta strandi178 – 1814Combined sources
Beta strandi184 – 1874Combined sources
Beta strandi190 – 1934Combined sources
Turni197 – 1993Combined sources
Helixi200 – 21112Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FTXX-ray1.90B154-213[»]
2FV4NMR-B138-213[»]
4GEQX-ray2.01B/D155-213[»]
ProteinModelPortaliQ04477.
SMRiQ04477. Positions 155-213.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04477.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili54 – 12370Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the SPC24 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGieuNOG07929.
HOGENOMiHOG000000760.
InParanoidiQ04477.
KOiK11557.
OMAiARISKEH.
OrthoDBiEOG76MKMF.

Family and domain databases

InterProiIPR013252. Ndc80_Spc24.
[Graphical view]
PfamiPF08286. Spc24. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04477-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQKDNLLDN PVEFLKEVRE SFDIQQDVDA MKRIRHDLDV IKEESEARIS
60 70 80 90 100
KEHSKVSESN KKLNAERINV AKLEGDLEYT NEESNEFGSK DELVKLLKDL
110 120 130 140 150
DGLERNIVSL RSELDEKMKL YLKDSEIIST PNGSKIKAKV IEPELEEQSA
160 170 180 190 200
VTPEANENIL KLKLYRSLGV ILDLENDQVL INRKNDGNID ILPLDNNLSD
210
FYKTKYIWER LGK
Length:213
Mass (Da):24,604
Last modified:November 1, 1997 - v1
Checksum:i8BE4B8619DD4C8FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49702 Genomic DNA. Translation: CAA89755.1.
BK006946 Genomic DNA. Translation: DAA10014.1.
PIRiS54579.
RefSeqiNP_013835.1. NM_001182617.1.

Genome annotation databases

EnsemblFungiiYMR117C; YMR117C; YMR117C.
GeneIDi855144.
KEGGisce:YMR117C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49702 Genomic DNA. Translation: CAA89755.1 .
BK006946 Genomic DNA. Translation: DAA10014.1 .
PIRi S54579.
RefSeqi NP_013835.1. NM_001182617.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FTX X-ray 1.90 B 154-213 [» ]
2FV4 NMR - B 138-213 [» ]
4GEQ X-ray 2.01 B/D 155-213 [» ]
ProteinModelPortali Q04477.
SMRi Q04477. Positions 155-213.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35293. 186 interactions.
DIPi DIP-893N.
IntActi Q04477. 62 interactions.
MINTi MINT-404120.
STRINGi 4932.YMR117C.

Proteomic databases

MaxQBi Q04477.
PaxDbi Q04477.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR117C ; YMR117C ; YMR117C .
GeneIDi 855144.
KEGGi sce:YMR117C.

Organism-specific databases

CYGDi YMR117c.
SGDi S000004723. SPC24.

Phylogenomic databases

eggNOGi euNOG07929.
HOGENOMi HOG000000760.
InParanoidi Q04477.
KOi K11557.
OMAi ARISKEH.
OrthoDBi EOG76MKMF.

Enzyme and pathway databases

BioCyci YEAST:G3O-32812-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q04477.
NextBioi 978540.

Gene expression databases

Genevestigatori Q04477.

Family and domain databases

InterProi IPR013252. Ndc80_Spc24.
[Graphical view ]
Pfami PF08286. Spc24. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control."
    Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E.
    EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TID3 AND NUF2.
  4. "The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation."
    Wigge P.A., Kilmartin J.V.
    J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
  5. "Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication."
    Le Masson I., Saveanu C., Chevalier A., Namane A., Gobin R., Fromont-Racine M., Jacquier A., Mann C.
    Mol. Microbiol. 43:1431-1443(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MPS2; NDC80 AND SPC25, SUBCELLULAR LOCATION.
  6. "The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity."
    McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T.
    Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE NDC80 COMPLEX.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association."
    Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U.
    Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  11. "Molecular organization of the Ndc80 complex, an essential kinetochore component."
    Wei R.R., Sorger P.K., Harrison S.C.
    Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.

Entry informationi

Entry nameiSPC24_YEAST
AccessioniPrimary (citable) accession number: Q04477
Secondary accession number(s): D6VZU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1750 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3