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Protein

Kinetochore protein SPC24

Gene

SPC24

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.3 Publications

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • microtubule nucleation Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32812-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein SPC24
Gene namesi
Name:SPC24
Ordered Locus Names:YMR117C
ORF Names:YM9718.16C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR117C.
SGDiS000004723. SPC24.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome, centromeric region Source: SGD
  • condensed nuclear chromosome kinetochore Source: SGD
  • Ndc80 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002032942 – 213Kinetochore protein SPC24Add BLAST212

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ04477.
PRIDEiQ04477.

PTM databases

iPTMnetiQ04477.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere. Can also interact with MPS2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-27228,EBI-27228
CDC12P324683EBI-27228,EBI-4182
MMR1Q063243EBI-27228,EBI-37386
MPS2P531598EBI-27228,EBI-23834
NDC80P4046019EBI-27228,EBI-25247
NUF2P338958EBI-27228,EBI-12377
SPC25P4001420EBI-27228,EBI-22458

Protein-protein interaction databases

BioGridi35293. 185 interactors.
DIPiDIP-893N.
IntActiQ04477. 62 interactors.
MINTiMINT-404120.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi157 – 167Combined sources11
Beta strandi170 – 173Combined sources4
Helixi174 – 176Combined sources3
Beta strandi178 – 181Combined sources4
Beta strandi184 – 187Combined sources4
Beta strandi190 – 193Combined sources4
Turni197 – 199Combined sources3
Helixi200 – 211Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FTXX-ray1.90B154-213[»]
2FV4NMR-B138-213[»]
4GEQX-ray2.01B/D155-213[»]
ProteinModelPortaliQ04477.
SMRiQ04477.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04477.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili54 – 123Sequence analysisAdd BLAST70

Sequence similaritiesi

Belongs to the SPC24 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000000760.
InParanoidiQ04477.
KOiK11557.
OMAiTDEATIM.
OrthoDBiEOG092C5OR0.

Family and domain databases

InterProiIPR013252. Ndc80_Spc24.
[Graphical view]
PfamiPF08286. Spc24. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKDNLLDN PVEFLKEVRE SFDIQQDVDA MKRIRHDLDV IKEESEARIS
60 70 80 90 100
KEHSKVSESN KKLNAERINV AKLEGDLEYT NEESNEFGSK DELVKLLKDL
110 120 130 140 150
DGLERNIVSL RSELDEKMKL YLKDSEIIST PNGSKIKAKV IEPELEEQSA
160 170 180 190 200
VTPEANENIL KLKLYRSLGV ILDLENDQVL INRKNDGNID ILPLDNNLSD
210
FYKTKYIWER LGK
Length:213
Mass (Da):24,604
Last modified:November 1, 1997 - v1
Checksum:i8BE4B8619DD4C8FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49702 Genomic DNA. Translation: CAA89755.1.
BK006946 Genomic DNA. Translation: DAA10014.1.
PIRiS54579.
RefSeqiNP_013835.1. NM_001182617.1.

Genome annotation databases

EnsemblFungiiYMR117C; YMR117C; YMR117C.
GeneIDi855144.
KEGGisce:YMR117C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49702 Genomic DNA. Translation: CAA89755.1.
BK006946 Genomic DNA. Translation: DAA10014.1.
PIRiS54579.
RefSeqiNP_013835.1. NM_001182617.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FTXX-ray1.90B154-213[»]
2FV4NMR-B138-213[»]
4GEQX-ray2.01B/D155-213[»]
ProteinModelPortaliQ04477.
SMRiQ04477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35293. 185 interactors.
DIPiDIP-893N.
IntActiQ04477. 62 interactors.
MINTiMINT-404120.

PTM databases

iPTMnetiQ04477.

Proteomic databases

MaxQBiQ04477.
PRIDEiQ04477.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR117C; YMR117C; YMR117C.
GeneIDi855144.
KEGGisce:YMR117C.

Organism-specific databases

EuPathDBiFungiDB:YMR117C.
SGDiS000004723. SPC24.

Phylogenomic databases

HOGENOMiHOG000000760.
InParanoidiQ04477.
KOiK11557.
OMAiTDEATIM.
OrthoDBiEOG092C5OR0.

Enzyme and pathway databases

BioCyciYEAST:G3O-32812-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ04477.
PROiQ04477.

Family and domain databases

InterProiIPR013252. Ndc80_Spc24.
[Graphical view]
PfamiPF08286. Spc24. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPC24_YEAST
AccessioniPrimary (citable) accession number: Q04477
Secondary accession number(s): D6VZU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1750 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.