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Q04477

- SPC24_YEAST

UniProt

Q04477 - SPC24_YEAST

Protein

Kinetochore protein SPC24

Gene

SPC24

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.3 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct
    3. structural constituent of cytoskeleton Source: SGD

    GO - Biological processi

    1. chromosome segregation Source: SGD
    2. microtubule nucleation Source: SGD
    3. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32812-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinetochore protein SPC24
    Gene namesi
    Name:SPC24
    Ordered Locus Names:YMR117C
    ORF Names:YM9718.16C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR117c.
    SGDiS000004723. SPC24.

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Associated with kinetochores.

    GO - Cellular componenti

    1. condensed nuclear chromosome, centromeric region Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD
    3. Ndc80 complex Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 213212Kinetochore protein SPC24PRO_0000203294Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ04477.
    PaxDbiQ04477.

    Expressioni

    Gene expression databases

    GenevestigatoriQ04477.

    Interactioni

    Subunit structurei

    Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere. Can also interact with MPS2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-27228,EBI-27228
    CDC12P324683EBI-27228,EBI-4182
    MMR1Q063243EBI-27228,EBI-37386
    MPS2P531598EBI-27228,EBI-23834
    NUF2P338958EBI-27228,EBI-12377
    SPC25P4001420EBI-27228,EBI-22458
    TID3P4046019EBI-27228,EBI-25247

    Protein-protein interaction databases

    BioGridi35293. 186 interactions.
    DIPiDIP-893N.
    IntActiQ04477. 62 interactions.
    MINTiMINT-404120.
    STRINGi4932.YMR117C.

    Structurei

    Secondary structure

    1
    213
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi157 – 16711
    Beta strandi170 – 1734
    Helixi174 – 1763
    Beta strandi178 – 1814
    Beta strandi184 – 1874
    Beta strandi190 – 1934
    Turni197 – 1993
    Helixi200 – 21112

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FTXX-ray1.90B154-213[»]
    2FV4NMR-B138-213[»]
    4GEQX-ray2.01B/D155-213[»]
    ProteinModelPortaliQ04477.
    SMRiQ04477. Positions 155-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ04477.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili54 – 12370Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SPC24 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGieuNOG07929.
    HOGENOMiHOG000000760.
    KOiK11557.
    OMAiARISKEH.
    OrthoDBiEOG76MKMF.

    Family and domain databases

    InterProiIPR013252. Ndc80_Spc24.
    [Graphical view]
    PfamiPF08286. Spc24. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04477-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQKDNLLDN PVEFLKEVRE SFDIQQDVDA MKRIRHDLDV IKEESEARIS    50
    KEHSKVSESN KKLNAERINV AKLEGDLEYT NEESNEFGSK DELVKLLKDL 100
    DGLERNIVSL RSELDEKMKL YLKDSEIIST PNGSKIKAKV IEPELEEQSA 150
    VTPEANENIL KLKLYRSLGV ILDLENDQVL INRKNDGNID ILPLDNNLSD 200
    FYKTKYIWER LGK 213
    Length:213
    Mass (Da):24,604
    Last modified:November 1, 1997 - v1
    Checksum:i8BE4B8619DD4C8FB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49702 Genomic DNA. Translation: CAA89755.1.
    BK006946 Genomic DNA. Translation: DAA10014.1.
    PIRiS54579.
    RefSeqiNP_013835.1. NM_001182617.1.

    Genome annotation databases

    EnsemblFungiiYMR117C; YMR117C; YMR117C.
    GeneIDi855144.
    KEGGisce:YMR117C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49702 Genomic DNA. Translation: CAA89755.1 .
    BK006946 Genomic DNA. Translation: DAA10014.1 .
    PIRi S54579.
    RefSeqi NP_013835.1. NM_001182617.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FTX X-ray 1.90 B 154-213 [» ]
    2FV4 NMR - B 138-213 [» ]
    4GEQ X-ray 2.01 B/D 155-213 [» ]
    ProteinModelPortali Q04477.
    SMRi Q04477. Positions 155-213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35293. 186 interactions.
    DIPi DIP-893N.
    IntActi Q04477. 62 interactions.
    MINTi MINT-404120.
    STRINGi 4932.YMR117C.

    Proteomic databases

    MaxQBi Q04477.
    PaxDbi Q04477.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR117C ; YMR117C ; YMR117C .
    GeneIDi 855144.
    KEGGi sce:YMR117C.

    Organism-specific databases

    CYGDi YMR117c.
    SGDi S000004723. SPC24.

    Phylogenomic databases

    eggNOGi euNOG07929.
    HOGENOMi HOG000000760.
    KOi K11557.
    OMAi ARISKEH.
    OrthoDBi EOG76MKMF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32812-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q04477.
    NextBioi 978540.

    Gene expression databases

    Genevestigatori Q04477.

    Family and domain databases

    InterProi IPR013252. Ndc80_Spc24.
    [Graphical view ]
    Pfami PF08286. Spc24. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control."
      Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E.
      EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TID3 AND NUF2.
    4. "The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation."
      Wigge P.A., Kilmartin J.V.
      J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
    5. "Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication."
      Le Masson I., Saveanu C., Chevalier A., Namane A., Gobin R., Fromont-Racine M., Jacquier A., Mann C.
      Mol. Microbiol. 43:1431-1443(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MPS2; NDC80 AND SPC25, SUBCELLULAR LOCATION.
    6. "The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity."
      McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T.
      Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NDC80 COMPLEX.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association."
      Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U.
      Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    11. "Molecular organization of the Ndc80 complex, an essential kinetochore component."
      Wei R.R., Sorger P.K., Harrison S.C.
      Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.

    Entry informationi

    Entry nameiSPC24_YEAST
    AccessioniPrimary (citable) accession number: Q04477
    Secondary accession number(s): D6VZU0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1750 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3