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Protein

Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR3

Gene

MGR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the mitochondrial inner membrane i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins. Together with MGR1, functions in an adapter complex that targets substrates to the i-AAA protease for degradation. Required for growth of cells lacking the mitochondrial genome.1 Publication

GO - Molecular functioni

  • misfolded protein binding Source: SGD

GO - Biological processi

  • misfolded or incompletely synthesized protein catabolic process Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32810-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR3
Alternative name(s):
Mitochondrial genome-required protein 3
Gene namesi
Name:MGR3
Synonyms:FMP24
Ordered Locus Names:YMR115W
ORF Names:YM9718.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR115W.
SGDiS000004721. MGR3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 77Mitochondrial matrix1 PublicationAdd BLAST77
Transmembranei78 – 95HelicalSequence analysisAdd BLAST18
Topological domaini96 – 501Mitochondrial intermembrane1 PublicationAdd BLAST406

GO - Cellular componenti

  • i-AAA complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Reduces proteolysis by YME1.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002032931 – 501Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR3Add BLAST501

Proteomic databases

MaxQBiQ04472.
PRIDEiQ04472.

Interactioni

Subunit structurei

Component of the mitochondrial inner membrane i-AAA protease supercomplex composed of MGR1, MGR3 and YME1. With MGR1, forms a subcomplex that binds to YME1 and to substrates to facilitate proteolysis.1 Publication

GO - Molecular functioni

  • misfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi35291. 88 interactors.
DIPiDIP-7624N.
IntActiQ04472. 1 interactor.
MINTiMINT-4497955.

Structurei

3D structure databases

ProteinModelPortaliQ04472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati109 – 144TPR 1Add BLAST36
Repeati154 – 187TPR 2Add BLAST34
Repeati386 – 420TPR 3Add BLAST35
Repeati440 – 473TPR 4Add BLAST34

Sequence similaritiesi

Belongs to the MGR3 family.Curated
Contains 4 TPR repeats.Curated

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000067630.
HOGENOMiHOG000248851.
InParanoidiQ04472.
OMAiRKGLWAE.
OrthoDBiEOG092C3NE2.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR011990. TPR-like_helical_dom.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04472-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLQGMRLSQ RLHKRHLFAS KILTWTTNPA HIRHLHDIRP PASNFNTQES
60 70 80 90 100
APIPESPANS PTRPQMAPKP NLKKKNRSLM YSIIGVSIVG LYFWFKSNSR
110 120 130 140 150
KQKLPLSAQK VWKEAIWQES DKMDFNYKEA LRRYIEALDE CDRSHVDLLS
160 170 180 190 200
DDYTRIELKI AEMYEKLNML EEAQNLYQEL LSRFFEALNV PGKVDESERG
210 220 230 240 250
EVLRKDLRIL IKSLEINKDI ESGKRKLLQH LLLAQEEILS KSPELKEFFE
260 270 280 290 300
NRKKKLSMVK DINRDPNDDF KTFVSEENIK FDEQGYMILD LEKNSSAWEP
310 320 330 340 350
FKEEFFTARD LYTAYCLSSK DIAAALSCKI TSVEWMVMAD MPPGQILLSQ
360 370 380 390 400
ANLGSLFYLQ AEKLEADLNQ LEQKKSKESN QELDMGTYIK AVRFVRKNRD
410 420 430 440 450
LCLERAQKCY DSVIAFAKRN RKIRFHVKDQ LDPSIAQSIA LSTYGMGVLS
460 470 480 490 500
LHEGVLAKAE KLFKDSITMA KETEFNELLA EAEKELEKTT VLKAAKKEGL

N
Length:501
Mass (Da):58,004
Last modified:November 1, 1997 - v1
Checksum:i994613B288C8DB7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49702 Genomic DNA. Translation: CAA89752.1.
BK006946 Genomic DNA. Translation: DAA10012.1.
PIRiS54576.
RefSeqiNP_013833.1. NM_001182615.1.

Genome annotation databases

EnsemblFungiiYMR115W; YMR115W; YMR115W.
GeneIDi855142.
KEGGisce:YMR115W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49702 Genomic DNA. Translation: CAA89752.1.
BK006946 Genomic DNA. Translation: DAA10012.1.
PIRiS54576.
RefSeqiNP_013833.1. NM_001182615.1.

3D structure databases

ProteinModelPortaliQ04472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35291. 88 interactors.
DIPiDIP-7624N.
IntActiQ04472. 1 interactor.
MINTiMINT-4497955.

Proteomic databases

MaxQBiQ04472.
PRIDEiQ04472.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR115W; YMR115W; YMR115W.
GeneIDi855142.
KEGGisce:YMR115W.

Organism-specific databases

EuPathDBiFungiDB:YMR115W.
SGDiS000004721. MGR3.

Phylogenomic databases

GeneTreeiENSGT00530000067630.
HOGENOMiHOG000248851.
InParanoidiQ04472.
OMAiRKGLWAE.
OrthoDBiEOG092C3NE2.

Enzyme and pathway databases

BioCyciYEAST:G3O-32810-MONOMER.

Miscellaneous databases

PROiQ04472.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR011990. TPR-like_helical_dom.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGR3_YEAST
AccessioniPrimary (citable) accession number: Q04472
Secondary accession number(s): D6VZT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.