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Q04472 (MGR3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR3
Alternative name(s):
Mitochondrial genome-required protein 3
Gene names
Name:MGR3
Synonyms:FMP24
Ordered Locus Names:YMR115W
ORF Names:YM9718.14
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the mitochondrial inner membrane i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins. Together with MGR1, functions in an adapter complex that targets substrates to the i-AAA protease for degradation. Required for growth of cells lacking the mitochondrial genome. Ref.7

Subunit structure

Component of the mitochondrial inner membrane i-AAA protease supercomplex composed of MGR1, MGR3 and YME1. With MGR1, forms a subcomplex that binds to YME1 and to substrates to facilitate proteolysis. Ref.7

Subcellular location

Mitochondrion inner membrane; Single-pass membrane protein Potential Ref.3 Ref.5 Ref.6 Ref.7.

Disruption phenotype

Reduces proteolysis by YME1. Ref.7

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the MGR3 family.

Contains 4 TPR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR3
PRO_0000203293

Regions

Topological domain1 – 7777Mitochondrial matrix Ref.7
Transmembrane78 – 9518Helical; Potential
Topological domain96 – 501406Mitochondrial intermembrane Ref.7
Repeat109 – 14436TPR 1
Repeat154 – 18734TPR 2
Repeat386 – 42035TPR 3
Repeat440 – 47334TPR 4

Sequences

Sequence LengthMass (Da)Tools
Q04472 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 994613B288C8DB7C

FASTA50158,004
        10         20         30         40         50         60 
MLLQGMRLSQ RLHKRHLFAS KILTWTTNPA HIRHLHDIRP PASNFNTQES APIPESPANS 

        70         80         90        100        110        120 
PTRPQMAPKP NLKKKNRSLM YSIIGVSIVG LYFWFKSNSR KQKLPLSAQK VWKEAIWQES 

       130        140        150        160        170        180 
DKMDFNYKEA LRRYIEALDE CDRSHVDLLS DDYTRIELKI AEMYEKLNML EEAQNLYQEL 

       190        200        210        220        230        240 
LSRFFEALNV PGKVDESERG EVLRKDLRIL IKSLEINKDI ESGKRKLLQH LLLAQEEILS 

       250        260        270        280        290        300 
KSPELKEFFE NRKKKLSMVK DINRDPNDDF KTFVSEENIK FDEQGYMILD LEKNSSAWEP 

       310        320        330        340        350        360 
FKEEFFTARD LYTAYCLSSK DIAAALSCKI TSVEWMVMAD MPPGQILLSQ ANLGSLFYLQ 

       370        380        390        400        410        420 
AEKLEADLNQ LEQKKSKESN QELDMGTYIK AVRFVRKNRD LCLERAQKCY DSVIAFAKRN 

       430        440        450        460        470        480 
RKIRFHVKDQ LDPSIAQSIA LSTYGMGVLS LHEGVLAKAE KLFKDSITMA KETEFNELLA 

       490        500 
EAEKELEKTT VLKAAKKEGL N 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[6]"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Mgr3p and Mgr1p are adaptors for the mitochondrial i-AAA protease complex."
Dunn C.D., Tamura Y., Sesaki H., Jensen R.E.
Mol. Biol. Cell 19:5387-5397(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49702 Genomic DNA. Translation: CAA89752.1.
BK006946 Genomic DNA. Translation: DAA10012.1.
PIRS54576.
RefSeqNP_013833.1. NM_001182615.1.

3D structure databases

ProteinModelPortalQ04472.
SMRQ04472. Positions 405-489.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35291. 89 interactions.
IntActQ04472. 1 interaction.
MINTMINT-4497955.
STRING4932.YMR115W.

Proteomic databases

PaxDbQ04472.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR115W; YMR115W; YMR115W.
GeneID855142.
KEGGsce:YMR115W.

Organism-specific databases

CYGDYMR115w.
SGDS000004721. MGR3.

Phylogenomic databases

eggNOGNOG275821.
GeneTreeENSGT00530000067630.
HOGENOMHOG000248851.
OMARKGLWAE.
OrthoDBEOG7712HV.

Enzyme and pathway databases

BioCycYEAST:G3O-32810-MONOMER.

Gene expression databases

GenevestigatorQ04472.

Family and domain databases

Gene3D1.25.40.10. 1 hit.
InterProIPR011990. TPR-like_helical.
[Graphical view]
ProtoNetSearch...

Other

NextBio978534.

Entry information

Entry nameMGR3_YEAST
AccessionPrimary (citable) accession number: Q04472
Secondary accession number(s): D6VZT8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families