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Protein

Endoglucanase 1

Gene

CMC1

Organism
Cryptococcus flavus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Has endoglucanase activity on carboxymethyl-cellulose (CMC).

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661Proton donorBy similarity
Active sitei275 – 2751NucleophileBy similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.
mycoCLAPiEGL5A_CRYFL.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 1 (EC:3.2.1.4)
Alternative name(s):
Carboxymethyl-cellulase 1
Short name:
CMCase 1
Short name:
Cellulase 1
Endo-1,4-beta-glucanase 1
Gene namesi
Name:CMC1
OrganismiCryptococcus flavus
Taxonomic identifieri5416 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesmitosporic TremellalesCryptococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Propeptidei17 – 3014Sequence AnalysisPRO_0000007855Add
BLAST
Chaini31 – 341311Endoglucanase 1PRO_0000007856Add
BLAST

Keywords - PTMi

Cleavage on pair of basic residues

Structurei

3D structure databases

ProteinModelPortaliQ04469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTATLLAAL SVLAGALAAP LAGDSALHRR SLPRLGGVNL AGCDFGIDIY
60 70 80 90 100
GNSGTPACPG TEQVGHFIAD GANLFRLPAG WQYLVGNNQA STSLAPDFFA
110 120 130 140 150
QYDALVQAVI SKGAYAIIDV HNYARWNGAI IGQGGPSNQD FANLWTLLAT
160 170 180 190 200
KVTSNDPNVI FGLMNEPHDL DVSTWAGSVQ AAVNAIRAAG ATSQYILIPG
210 220 230 240 250
TGFTNANAWF QGQDNALLGV TDPVGGTDKL LLDVHRYNDV DFSGTHAECT
260 270 280 290 300
TNSLDVLSSL DSWLKGNGRK AIVSETGGGH TTSCETDLGE FLNGIKEDYP
310 320 330 340
SVLGFAVWAA GSFDPSYVLS ITPTNGVDNQ LFDIAVKPNL P
Length:341
Mass (Da):35,717
Last modified:October 1, 1996 - v1
Checksum:i4A62E45C952DD120
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13967 mRNA. Translation: BAA03070.1.
S45137 Genomic DNA. Translation: AAC60541.1.
PIRiJC1201.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13967 mRNA. Translation: BAA03070.1.
S45137 Genomic DNA. Translation: AAC60541.1.
PIRiJC1201.

3D structure databases

ProteinModelPortaliQ04469.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.
mycoCLAPiEGL5A_CRYFL.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and molecular analysis of cDNA encoding a carboxymethylcellulase of the yeast Cryptococcus flavus."
    Cui Z., Mochizuki D., Matsuno Y., Nakamura T., Liu Y., Hatano T., Fukui S., Miyakawa T.
    Biosci. Biotechnol. Biochem. 56:1230-1235(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiGUN1_CRYFL
AccessioniPrimary (citable) accession number: Q04469
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.