Reviewed, UniProtKB/Swiss-Prot Q04469 (GUN1_CRYFL)

Names and origin

Protein names

Recommended name:
    Endoglucanase 1
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase 1
    Carboxymethyl-cellulase 1
      Short name=CMCase 1
      Short name=Cellulase 1

Gene names

Name:

CMC1

Organism

Cryptococcus flavus

Taxonomic identifier

5416 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › mitosporic Tremellales › Cryptococcus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has endoglucanase activity on carboxymethyl-cellulose (CMC).
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Cleavage on pair of basic residues
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 30	14	Potential		PRO_0000007855
	Chain	31 – 341	311	Endoglucanase 1		PRO_0000007856
Sites
	Active site	166	1	Proton donor By similarity
	Active site	275	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04469-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4A62E45C952DD120	FASTA	341	35,717
	10 20 30 40 50 60 MKTATLLAAL SVLAGALAAP LAGDSALHRR SLPRLGGVNL AGCDFGIDIY GNSGTPACPG 70 80 90 100 110 120 TEQVGHFIAD GANLFRLPAG WQYLVGNNQA STSLAPDFFA QYDALVQAVI SKGAYAIIDV 130 140 150 160 170 180 HNYARWNGAI IGQGGPSNQD FANLWTLLAT KVTSNDPNVI FGLMNEPHDL DVSTWAGSVQ 190 200 210 220 230 240 AAVNAIRAAG ATSQYILIPG TGFTNANAWF QGQDNALLGV TDPVGGTDKL LLDVHRYNDV 250 260 270 280 290 300 DFSGTHAECT TNSLDVLSSL DSWLKGNGRK AIVSETGGGH TTSCETDLGE FLNGIKEDYP 310 320 330 340 SVLGFAVWAA GSFDPSYVLS ITPTNGVDNQ LFDIAVKPNL P

References

[1]

"Cloning and molecular analysis of cDNA encoding a carboxymethylcellulase of the yeast Cryptococcus flavus." Cui Z., Mochizuki D., Matsuno Y., Nakamura T., Liu Y., Hatano T., Fukui S., Miyakawa T. Biosci. Biotechnol. Biochem. 56:1230-1235(1992) [PubMed: 1368837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13967 mRNA. Translation: BAA03070.1. S45137 Genomic DNA. Translation: AAC60541.1.
PIR	JC1201.
3D structure databases
HSSP	HSSP built from PDB template 1H1N based on UniProtKB Q8TG26.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Enzyme and pathway databases
BRENDA	3.2.1.4. 276441.
Family and domain databases
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_CRYFL

Accession

Primary (citable) accession number: Q04469

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)