Q04468 (TCMO_HELTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trans-cinnamate 4-monooxygenase
EC=1.14.13.11

Alternative name(s):
Cinnamic acid 4-hydroxylase
Short name=C4H
Short name=CA4H
Cytochrome P450 73
Cytochrome P450C4H

Gene names

Name:	CYP73A1
Synonyms:	CYP73

Organism

Helianthus tuberosus (Jerusalem artichoke) (Helianthus tomentosus)

Taxonomic identifier

4233 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae alliance › Heliantheae › Helianthus

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.
Induction	By light, wounding, infection, exposure to xenobiotics and fungal elicitor.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Trans-cinnamate 4-monooxygenase		PRO_0000052246
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04468 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 9BA009F5554BAFD7	FASTA	505	57,920
	10 20 30 40 50 60 MDLLLIEKTL VALFAAIIGA ILISKLRGKK FKLPPGPIPV PIFGNWLQVG DDLNHRNLTD 70 80 90 100 110 120 LAKRFGEILL LRMGQRNLVV VSSPELAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT 130 140 150 160 170 180 VYGEHWRKMR RIMTVPFFTN KVVQQYRYGW EAEAAAVVDD VKKNPAAATE GIVIRRRLQL 190 200 210 220 230 240 MMYNNMFRIM FDRRFESEDD PLFLKLKALN GERSRLAQSF EYNYGDFIPI LRPFLRNYLK 250 260 270 280 290 300 LCKEVKDKRI QLFKDYFVDE RKKIGSTKKM DNNQLKCAID HILEAKEKGE INEDNVLYIV 310 320 330 340 350 360 ENINVAAIET TLWSIEWGIA ELVNHPEIQA KLRHELDTKL GPGVQITEPD VQNLPYLQAV 370 380 390 400 410 420 VKETLRLRMA IPLLVPHMNL HDAKLGGFDI PAESKILVNA WWLANNPDQW KKPEEFRPER 430 440 450 460 470 480 FLEEEAKVEA NGNDFRYLPF GVGRRSCPGI ILALPILGIT IGRLVQNFEL LPPPGQSKID 490 500 TDEKGGQFSL HILKHSTIVA KPRSF

References

[1]

"Isolation and sequence of a cDNA encoding the Jerusalem artichoke cinnamate 4-hydroxylase, a major plant cytochrome P450 involved in the general phenylpropanoid pathway." Teutsch H.G., Hasenfratz M., Lesot A., Stoltz C., Garnier J.-M., Jeltsch J.-M., Durst F., Werck-Reichhart D. Proc. Natl. Acad. Sci. U.S.A. 90:4102-4106(1993) [PubMed: 8097885] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-21. Strain: cv. Blanc commun. Tissue: Tuberous root.

[2]

"Characterization of recombinant plant cinnamate 4-hydroxylase produced in yeast. Kinetic and spectral properties of the major plant P450 of the phenylpropanoid pathway." Urban P., Werck-Reichhart D., Teutsch H.G., Durst F., Regnier S., Kazmaier M., Pompon D. Eur. J. Biochem. 222:843-850(1994) [PubMed: 8026495] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z17369 mRNA. Translation: CAA78982.1.
PIR	A47454.
3D structure databases
ProteinModelPortal	Q04468.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_HELTU

Accession

Primary (citable) accession number: Q04468

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 28, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program