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Q04467 (IDHP_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP], mitochondrial

Short name=IDH
EC=1.1.1.42
Alternative name(s):
ICD-M
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene names
Name:IDH2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Homodimer.

Subcellular location

Mitochondrion.

Post-translational modification

Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3 By similarity.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Mitochondrion Ref.1
Chain40 – 452413Isocitrate dehydrogenase [NADP], mitochondrial
PRO_0000014419

Regions

Nucleotide binding115 – 1173NADP By similarity
Nucleotide binding349 – 3546NADP By similarity
Region134 – 1407Substrate binding By similarity

Sites

Metal binding2911Magnesium or manganese By similarity
Metal binding3141Magnesium or manganese By similarity
Binding site1171Substrate By similarity
Binding site1221NADP By similarity
Binding site1491Substrate By similarity
Binding site1721Substrate By similarity
Binding site2991NADP By similarity
Binding site3671NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1791Critical for catalysis By similarity
Site2511Critical for catalysis By similarity

Amino acid modifications

Modified residue451N6-acetyllysine By similarity
Modified residue481N6-acetyllysine By similarity
Modified residue671N6-acetyllysine By similarity
Modified residue691N6-acetyllysine By similarity
Modified residue801N6-acetyllysine; alternate By similarity
Modified residue801N6-succinyllysine; alternate By similarity
Modified residue1061N6-acetyllysine; alternate By similarity
Modified residue1061N6-succinyllysine; alternate By similarity
Modified residue1551N6-acetyllysine By similarity
Modified residue1661N6-acetyllysine; alternate By similarity
Modified residue1661N6-succinyllysine; alternate By similarity
Modified residue1801N6-acetyllysine; alternate By similarity
Modified residue1801N6-succinyllysine; alternate By similarity
Modified residue1931N6-acetyllysine; alternate By similarity
Modified residue1931N6-succinyllysine; alternate By similarity
Modified residue1991N6-acetyllysine By similarity
Modified residue2561N6-acetyllysine; alternate By similarity
Modified residue2561N6-succinyllysine; alternate By similarity
Modified residue2631N6-acetyllysine By similarity
Modified residue2721N6-acetyllysine By similarity
Modified residue2751N6-acetyllysine By similarity
Modified residue2801N6-acetyllysine By similarity
Modified residue2821N6-acetyllysine; alternate By similarity
Modified residue2821N6-succinyllysine; alternate By similarity
Modified residue3841N6-acetyllysine; alternate By similarity
Modified residue3841N6-succinyllysine; alternate By similarity
Modified residue4001N6-acetyllysine By similarity
Modified residue4131N6-acetyllysine By similarity
Modified residue4421N6-acetyllysine By similarity

Experimental info

Sequence conflict241A → R in CAA49207. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q04467 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: FAB87D3C624E0C71

FASTA45250,739
        10         20         30         40         50         60 
MAGYLRVVRS LCRASGSGSA WAPAALTAPN LQEQPRRHYA DKRIKVAKPV VEMDGDEMTR 

        70         80         90        100        110        120 
IIWQFIKEKL ILPHVDVQLK YFDLGLPNRD QTNDQVTIDS ALATQKYSVA VKCATITPDE 

       130        140        150        160        170        180 
ARVEEFKLKK MWKSPNGTIR NILGGTVFRE PIICKNIPRL VPGWTKPITI GRHAHGDQYK 

       190        200        210        220        230        240 
ATDFVVDRAG TFKVVFTPKD GSGPKEWEVY NFPAGGVGMG MYNTDESISG FAHSCFQYAI 

       250        260        270        280        290        300 
QKKWPLYMST KNTILKAYDG RFKDIFQAIF EKHYKTEFDK HKIWYEHRLI DDMVAQVLKS 

       310        320        330        340        350        360 
SGGFVWACKN YDGDVQSDIL AQGFGSLGLM TSVLVCPDGK TIEAEAAHGT VTRHYREHQK 

       370        380        390        400        410        420 
GRPTSTNPIA SIFAWTRGLE HRGKLDGNQD LIRFAQTLEK VCVETVESGA MTKDLAGCIH 

       430        440        450 
GLSNVKLNEH FLNTSDFLDT IKSNLDRALG QQ 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a cDNA encoding bovine mitochondrial NADP(+)-specific isocitrate dehydrogenase and structural comparison with its isoenzymes from different species."
Huh T.-L., Ryu J.-H., Huh J.-W., Sung H.-C., Oh I.-U., Song B.J., Veech R.L.
Biochem. J. 292:705-710(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 40-55.
Tissue: Kidney.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Ileum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69432 mRNA. Translation: CAA49207.1.
BC102509 mRNA. Translation: AAI02510.1.
PIRS33859.
RefSeqNP_786984.1. NM_175790.2.
UniGeneBt.5520.

3D structure databases

ProteinModelPortalQ04467.
SMRQ04467. Positions 40-452.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ04467. 1 interaction.
STRING9913.ENSBTAP00000018741.

Proteomic databases

PaxDbQ04467.
PRIDEQ04467.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000018741; ENSBTAP00000018741; ENSBTAG00000014093.
GeneID327669.
KEGGbta:327669.

Organism-specific databases

CTD3418.

Phylogenomic databases

eggNOGCOG0538.
GeneTreeENSGT00390000012547.
HOGENOMHOG000019858.
HOVERGENHBG006119.
InParanoidQ04467.
KOK00031.
OMAGSGPTWA.
OrthoDBEOG7QNVKS.
TreeFamTF300428.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20810134.

Entry information

Entry nameIDHP_BOVIN
AccessionPrimary (citable) accession number: Q04467
Secondary accession number(s): Q3ZCD4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 30, 2006
Last modified: April 16, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families