Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q04467

- IDHP_BOVIN

UniProt

Q04467 - IDHP_BOVIN

Protein

Isocitrate dehydrogenase [NADP], mitochondrial

Gene

IDH2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (30 May 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171SubstrateBy similarity
    Binding sitei122 – 1221NADPBy similarity
    Binding sitei149 – 1491SubstrateBy similarity
    Binding sitei172 – 1721SubstrateBy similarity
    Sitei179 – 1791Critical for catalysisBy similarity
    Sitei251 – 2511Critical for catalysisBy similarity
    Metal bindingi291 – 2911Magnesium or manganeseBy similarity
    Binding sitei299 – 2991NADPBy similarity
    Metal bindingi314 – 3141Magnesium or manganeseBy similarity
    Binding sitei367 – 3671NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi115 – 1173NADPBy similarity
    Nucleotide bindingi349 – 3546NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. 2-oxoglutarate metabolic process Source: UniProtKB
    2. glyoxylate cycle Source: UniProtKB-KW
    3. isocitrate metabolic process Source: UniProtKB
    4. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiREACT_227707. Citric acid cycle (TCA cycle).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP], mitochondrial (EC:1.1.1.42)
    Short name:
    IDH
    Alternative name(s):
    ICD-M
    IDP
    NADP(+)-specific ICDH
    Oxalosuccinate decarboxylase
    Gene namesi
    Name:IDH2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 21

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: AgBase
    2. mitochondrion Source: AgBase

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939Mitochondrion1 PublicationAdd
    BLAST
    Chaini40 – 452413Isocitrate dehydrogenase [NADP], mitochondrialPRO_0000014419Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei45 – 451N6-acetyllysineBy similarity
    Modified residuei48 – 481N6-acetyllysineBy similarity
    Modified residuei67 – 671N6-acetyllysineBy similarity
    Modified residuei69 – 691N6-acetyllysineBy similarity
    Modified residuei80 – 801N6-acetyllysine; alternateBy similarity
    Modified residuei80 – 801N6-succinyllysine; alternateBy similarity
    Modified residuei106 – 1061N6-acetyllysine; alternateBy similarity
    Modified residuei106 – 1061N6-succinyllysine; alternateBy similarity
    Modified residuei155 – 1551N6-acetyllysineBy similarity
    Modified residuei166 – 1661N6-acetyllysine; alternateBy similarity
    Modified residuei166 – 1661N6-succinyllysine; alternateBy similarity
    Modified residuei180 – 1801N6-acetyllysine; alternateBy similarity
    Modified residuei180 – 1801N6-succinyllysine; alternateBy similarity
    Modified residuei193 – 1931N6-acetyllysine; alternateBy similarity
    Modified residuei193 – 1931N6-succinyllysine; alternateBy similarity
    Modified residuei199 – 1991N6-acetyllysineBy similarity
    Modified residuei256 – 2561N6-acetyllysine; alternateBy similarity
    Modified residuei256 – 2561N6-succinyllysine; alternateBy similarity
    Modified residuei263 – 2631N6-acetyllysineBy similarity
    Modified residuei272 – 2721N6-acetyllysineBy similarity
    Modified residuei275 – 2751N6-acetyllysineBy similarity
    Modified residuei280 – 2801N6-acetyllysineBy similarity
    Modified residuei282 – 2821N6-acetyllysine; alternateBy similarity
    Modified residuei282 – 2821N6-succinyllysine; alternateBy similarity
    Modified residuei384 – 3841N6-acetyllysine; alternateBy similarity
    Modified residuei384 – 3841N6-succinyllysine; alternateBy similarity
    Modified residuei400 – 4001N6-acetyllysineBy similarity
    Modified residuei413 – 4131N6-acetyllysineBy similarity
    Modified residuei442 – 4421N6-acetyllysineBy similarity

    Post-translational modificationi

    Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3 By similarity.By similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ04467.
    PRIDEiQ04467.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    IntActiQ04467. 1 interaction.
    STRINGi9913.ENSBTAP00000018741.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04467.
    SMRiQ04467. Positions 40-452.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni134 – 1407Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0538.
    GeneTreeiENSGT00390000012547.
    HOGENOMiHOG000019858.
    HOVERGENiHBG006119.
    InParanoidiQ04467.
    KOiK00031.
    OMAiCFQYAIG.
    OrthoDBiEOG7QNVKS.
    TreeFamiTF300428.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04467-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGYLRVVRS LCRASGSGSA WAPAALTAPN LQEQPRRHYA DKRIKVAKPV    50
    VEMDGDEMTR IIWQFIKEKL ILPHVDVQLK YFDLGLPNRD QTNDQVTIDS 100
    ALATQKYSVA VKCATITPDE ARVEEFKLKK MWKSPNGTIR NILGGTVFRE 150
    PIICKNIPRL VPGWTKPITI GRHAHGDQYK ATDFVVDRAG TFKVVFTPKD 200
    GSGPKEWEVY NFPAGGVGMG MYNTDESISG FAHSCFQYAI QKKWPLYMST 250
    KNTILKAYDG RFKDIFQAIF EKHYKTEFDK HKIWYEHRLI DDMVAQVLKS 300
    SGGFVWACKN YDGDVQSDIL AQGFGSLGLM TSVLVCPDGK TIEAEAAHGT 350
    VTRHYREHQK GRPTSTNPIA SIFAWTRGLE HRGKLDGNQD LIRFAQTLEK 400
    VCVETVESGA MTKDLAGCIH GLSNVKLNEH FLNTSDFLDT IKSNLDRALG 450
    QQ 452
    Length:452
    Mass (Da):50,739
    Last modified:May 30, 2006 - v2
    Checksum:iFAB87D3C624E0C71
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241A → R in CAA49207. (PubMed:8318002)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69432 mRNA. Translation: CAA49207.1.
    BC102509 mRNA. Translation: AAI02510.1.
    PIRiS33859.
    RefSeqiNP_786984.1. NM_175790.2.
    UniGeneiBt.5520.

    Genome annotation databases

    EnsembliENSBTAT00000018741; ENSBTAP00000018741; ENSBTAG00000014093.
    GeneIDi327669.
    KEGGibta:327669.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69432 mRNA. Translation: CAA49207.1 .
    BC102509 mRNA. Translation: AAI02510.1 .
    PIRi S33859.
    RefSeqi NP_786984.1. NM_175790.2.
    UniGenei Bt.5520.

    3D structure databases

    ProteinModelPortali Q04467.
    SMRi Q04467. Positions 40-452.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q04467. 1 interaction.
    STRINGi 9913.ENSBTAP00000018741.

    Proteomic databases

    PaxDbi Q04467.
    PRIDEi Q04467.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000018741 ; ENSBTAP00000018741 ; ENSBTAG00000014093 .
    GeneIDi 327669.
    KEGGi bta:327669.

    Organism-specific databases

    CTDi 3418.

    Phylogenomic databases

    eggNOGi COG0538.
    GeneTreei ENSGT00390000012547.
    HOGENOMi HOG000019858.
    HOVERGENi HBG006119.
    InParanoidi Q04467.
    KOi K00031.
    OMAi CFQYAIG.
    OrthoDBi EOG7QNVKS.
    TreeFami TF300428.

    Enzyme and pathway databases

    Reactomei REACT_227707. Citric acid cycle (TCA cycle).

    Miscellaneous databases

    NextBioi 20810134.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a cDNA encoding bovine mitochondrial NADP(+)-specific isocitrate dehydrogenase and structural comparison with its isoenzymes from different species."
      Huh T.-L., Ryu J.-H., Huh J.-W., Sung H.-C., Oh I.-U., Song B.J., Veech R.L.
      Biochem. J. 292:705-710(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 40-55.
      Tissue: Kidney.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.

    Entry informationi

    Entry nameiIDHP_BOVIN
    AccessioniPrimary (citable) accession number: Q04467
    Secondary accession number(s): Q3ZCD4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: May 30, 2006
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3