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Protein

Valine--tRNA ligase

Gene

Vars

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).

Enzyme regulationi

Can be regulated by protein kinase C-dependent phosphorylation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei865ATPBy similarity1

GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • valine-tRNA ligase activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligase (EC:6.1.1.9)
Alternative name(s):
Valyl-tRNA synthetase
Short name:
ValRS
Gene namesi
Name:Vars
Synonyms:Vars2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi3950. Vars.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001062552 – 1264Valine--tRNA ligaseAdd BLAST1263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei437PhosphoserineBy similarity1
Modified residuei527PhosphoserineBy similarity1
Modified residuei645N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ04462.
PRIDEiQ04462.

PTM databases

iPTMnetiQ04462.
PhosphoSitePlusiQ04462.
SwissPalmiQ04462.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000867.
ExpressionAtlasiQ04462. baseline and differential.
GenevisibleiQ04462. RN.

Interactioni

Subunit structurei

Forms high-molecular-mass aggregates with elongation factor 1.

Protein-protein interaction databases

IntActiQ04462. 2 interactors.
MINTiMINT-4596172.
STRINGi10116.ENSRNOP00000001160.

Structurei

3D structure databases

ProteinModelPortaliQ04462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 219GST C-terminalAdd BLAST131

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi344 – 354"HIGH" regionAdd BLAST11
Motifi862 – 866"KMSKS" region5

Sequence similaritiesi

Contains 1 GST C-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0432. Eukaryota.
KOG0867. Eukaryota.
COG0525. LUCA.
GeneTreeiENSGT00550000074727.
HOGENOMiHOG000020094.
HOVERGENiHBG017878.
InParanoidiQ04462.
KOiK01873.
OMAiPLSIFGW.
OrthoDBiEOG091G01XH.
PhylomeDBiQ04462.
TreeFamiTF300648.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00043. GST_C. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILYVSPHP DAFPSLRALI AARYGEAGDG PGWGGPHPRI CLQPPPSSRT
60 70 80 90 100
PFPPPRLPAL EQGPGGLWVW GAPAVAQLLW PAGLGGPGGS RAAVLVQQWV
110 120 130 140 150
SYADTELTPA ACGATLPALG LRGPGQDPQA ALGALGKALN PLEEWLRLHT
160 170 180 190 200
YLAGDAPTLA DLAAVTALLL PFRYVLDPSA RRIWGNVTRW FNTCVRQPEF
210 220 230 240 250
RAVLGEVVLY SGARSVTQQP GSEITAPQKT AAQLKKEAKK REKLEKFQQK
260 270 280 290 300
QKTQQQQPAH GEKKPKPEKK EKRDPGVITY DLPTPPGEKK DVSGTMPDSY
310 320 330 340 350
SPQYVEAAWY PWWERQGFFK PEYGRPSVSA PNPRGVFMMC IPPPNVTGSL
360 370 380 390 400
HLGHALTNAI QDSLTRWHRM RGETTLWNPG CDHAGIATQV VVEKKLWKER
410 420 430 440 450
GLNRHQLGRE AFLQEVWKWK AEKGDRIYHQ LKKLGSSLDW DRACFTMDPK
460 470 480 490 500
LSATVTEAFV RLHEEGVIYR STRLVNWSCT LNSAISDIEV DKKELTGRTL
510 520 530 540 550
LSVPGYKEKV EFGVLVSFAY KVQGSDSDEE VVVATTRIET MLGDVAVAVH
560 570 580 590 600
PKDPRYQHLK GKSVVHPFLS RSLPIVFDDF VDMEFGTGAV KITPAHDQND
610 620 630 640 650
YEVGQRHRLE AISIMDSKGA LVNVPPPFLG LPRFEARKAV LAALKEQGLF
660 670 680 690 700
RGIKDNPMVV PLCNRSKDVV EPLLRPQWYV RCGEMAQAAS AAVTRGDLRI
710 720 730 740 750
LPEAHQRTWH SWMDNIRDWC ISRQLWWGHR IPAYFITVHD PAVPPGEDPD
760 770 780 790 800
GRYWVSGRTE AEAREKAARE FGVSPDKISL QQDEDVLDTW FSSGLFPFSI
810 820 830 840 850
FGWPNQSEDL SVFYPGTLLE TGHDILFFWV ARMVMLGLKL TEKLPFREVY
860 870 880 890 900
LHAIVRDAHG RKMSKSLGNV IDPLDVIHGV SLQGLHDQLL NSNLDPSEVE
910 920 930 940 950
KAKEGQRADF PAGIPECGTD ALRFGLCAYT SQGRDINLDV NRILGYRHFC
960 970 980 990 1000
NKLWNATKFA LRGLGKGFVP SPTSKPEGHE SLVDRWIRSR LAEAVRLSNE
1010 1020 1030 1040 1050
GFQAYDFPAV TTAQYSFWLY ELCDVYLECL KPVLNGVDQV AADCARQTLY
1060 1070 1080 1090 1100
TCLDVGLRLL SPFMPFVTEE LFQRLPRRTP NAPASLCVTP YPEPSECSWK
1110 1120 1130 1140 1150
DPEAEAALEL ALSITRAVRS LRADYNLTRT RPDCFLEVAD EATGALASAV
1160 1170 1180 1190 1200
SAYVQTLASA GVVAVLALGA PAPQGCAVAV ASDRCSIHLQ LQGLVDPARE
1210 1220 1230 1240 1250
LGKLQAKRSE AQRQAQRLQE RRAASGYSAK VPLEVQEADE VKLQQTEAEL
1260
RKVDEAIALF QKML
Length:1,264
Mass (Da):140,368
Last modified:August 30, 2005 - v2
Checksum:i11DE06D1D04FAE00
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti498 – 500RTL → EFR (PubMed:8428657).Curated3
Sequence conflicti520Y → C in AAA42320 (PubMed:8428657).Curated1
Sequence conflicti653I → V in AAA42320 (PubMed:8428657).Curated1
Sequence conflicti842E → G in AAA42320 (PubMed:8428657).Curated1
Sequence conflicti1056 – 1057GL → AV in AAA42320 (PubMed:8428657).Curated2
Sequence conflicti1089 – 1091TPY → RNS in AAA42320 (PubMed:8428657).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883045 Genomic DNA. Translation: CAE83981.1.
M98327 mRNA. Translation: AAA42320.1.
PIRiPN0473.
RefSeqiNP_445744.1. NM_053292.1.
XP_006256109.1. XM_006256047.3.
UniGeneiRn.12314.

Genome annotation databases

EnsembliENSRNOT00000001160; ENSRNOP00000001160; ENSRNOG00000000867.
ENSRNOT00000087861; ENSRNOP00000072696; ENSRNOG00000000867.
GeneIDi25009.
KEGGirno:25009.
UCSCiRGD:3950. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883045 Genomic DNA. Translation: CAE83981.1.
M98327 mRNA. Translation: AAA42320.1.
PIRiPN0473.
RefSeqiNP_445744.1. NM_053292.1.
XP_006256109.1. XM_006256047.3.
UniGeneiRn.12314.

3D structure databases

ProteinModelPortaliQ04462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ04462. 2 interactors.
MINTiMINT-4596172.
STRINGi10116.ENSRNOP00000001160.

PTM databases

iPTMnetiQ04462.
PhosphoSitePlusiQ04462.
SwissPalmiQ04462.

Proteomic databases

PaxDbiQ04462.
PRIDEiQ04462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001160; ENSRNOP00000001160; ENSRNOG00000000867.
ENSRNOT00000087861; ENSRNOP00000072696; ENSRNOG00000000867.
GeneIDi25009.
KEGGirno:25009.
UCSCiRGD:3950. rat.

Organism-specific databases

CTDi7407.
RGDi3950. Vars.

Phylogenomic databases

eggNOGiKOG0432. Eukaryota.
KOG0867. Eukaryota.
COG0525. LUCA.
GeneTreeiENSGT00550000074727.
HOGENOMiHOG000020094.
HOVERGENiHBG017878.
InParanoidiQ04462.
KOiK01873.
OMAiPLSIFGW.
OrthoDBiEOG091G01XH.
PhylomeDBiQ04462.
TreeFamiTF300648.

Miscellaneous databases

PROiQ04462.

Gene expression databases

BgeeiENSRNOG00000000867.
ExpressionAtlasiQ04462. baseline and differential.
GenevisibleiQ04462. RN.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00043. GST_C. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYVC_RAT
AccessioniPrimary (citable) accession number: Q04462
Secondary accession number(s): Q6MG65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: August 30, 2005
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.