Q04458 (HFD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative fatty aldehyde dehydrogenase HFD1
EC=1.2.1.-

Gene names

Name:	HFD1
Ordered Locus Names:	YMR110C
ORF Names:	YM9718.09C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein Potential. Endosome membrane; Single-pass membrane protein Potential. Cytoplasmic granule membrane; Single-pass membrane protein Potential Ref.3 Ref.5 Ref.6 Ref.7.
Miscellaneous	Present with 2930 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Endosome Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular aldehyde metabolic process Inferred by curator Ref.7. Source: SGD
Cellular component	endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to mitochondrial outer membrane Inferred from direct assay Ref.6. Source: SGD lipid particle Inferred from direct assay Ref.3. Source: SGD
Molecular function	aldehyde dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Putative fatty aldehyde dehydrogenase HFD1		PRO_0000056597
Regions
	Transmembrane	134 – 152	19	Helical; Potential
Sites
	Active site	236	1	By similarity
	Active site	273	1	By similarity
Amino acid modifications
	Modified residue	111	1	Phosphoserine Ref.8 Ref.9 Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q04458 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 6CAF4BFCF963AF88	FASTA	532	59,979
	10 20 30 40 50 60 MSNDGSKILN YTPVSKIDEI VEISRNFFFE KQLKLSHENN PRKKDLEFRQ LQLKKLYYAV 70 80 90 100 110 120 KDHEEELIDA MYKDFHRNKI ESVLNETTKL MNDILHLIEI LPKLIKPRRV SDSSPPFMFG 130 140 150 160 170 180 KTIVEKISRG SVLIIAPFNF PLLLAFAPLA AALAAGNTIV LKPSELTPHT AVVMENLLTT 190 200 210 220 230 240 AGFPDGLIQV VQGAIDETTR LLDCGKFDLI FYTGSPRVGS IVAEKAAKSL TPCVLELGGK 250 260 270 280 290 300 SPTFITENFK ASNIKIALKR IFFGAFGNSG QICVSPDYLL VHKSIYPKVI KECESVLNEF 310 320 330 340 350 360 YPSFDEQTDF TRMIHEPAYK KAVASINSTN GSKIVPSKIS INSDTEDLCL VPPTIVYNIG 370 380 390 400 410 420 WDDPLMKQEN FAPVLPIIEY EDLDETINKI IEEHDTPLVQ YIFSDSQTEI NRILTRLRSG 430 440 450 460 470 480 DCVVGDTVIH VGITDAPFGG IGTSGYGNYG GYYGFNTFSH ERTIFKQPYW NDFTLFMRYP 490 500 510 520 530 PNSAQKEKLV RFAMERKPWF DRNGNNKWGL RQYFSLSAAV ILISTIYAHC SS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[6]	"Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae." Burri L., Vascotto K., Gentle I.E., Chan N.C., Beilharz T., Stapleton D.I., Ramage L., Lithgow T. FEBS J. 273:1507-1515(2006) [PubMed: 16689936] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[7]	"Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins." Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C. Mol. Biol. Cell 17:1436-1450(2006) [PubMed: 16407407] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49702 Genomic DNA. Translation: CAA89746.1. BK006946 Genomic DNA. Translation: DAA10007.1.
PIR	S54571.
RefSeq	NP_013828.1. NM_001182610.1.
3D structure databases
ProteinModelPortal	Q04458.
SMR	Q04458. Positions 10-468.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4437N.
IntAct	Q04458. 10 interactions.
MINT	MINT-570953.
STRING	Q04458.
Proteomic databases
PeptideAtlas	Q04458.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR110C; YMR110C; YMR110C.
GeneID	855137.
KEGG	sce:YMR110C.
NMPDR	fig|4932.3.peg.4876.
Organism-specific databases
CYGD	YMR110c.
SGD	S000004716. HFD1.
Phylogenomic databases
eggNOG	fuNOG05022.
GeneTree	EFGT00050000002267.
HOGENOM	HBG752218.
OMA	HEWVKPE.
OrthoDB	EOG4XKZGC.
Gene expression databases
ArrayExpress	Q04458.
Genevestigator	Q04458.
GermOnline	YMR110C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR012394. Aldehyde_DH_NAD(P). [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699:SF15. ALDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF036492. ALDH. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. False negative. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978519.

Entry information

Entry name

HFD1_YEAST

Accession

Primary (citable) accession number: Q04458
Secondary accession number(s): D6VZT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program