Reviewed, UniProtKB/Swiss-Prot Q04458 (HFD1_YEAST)

Names and origin

Protein names

Recommended name:
    Putative fatty aldehyde dehydrogenase HFD1
    EC=1.2.1.-

Gene names

Name:	HFD1
Ordered Locus Names:	YMR110C
ORF Names:	YM9718.09C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein Potential. Endosome membrane; Single-pass membrane protein Potential. Cytoplasmic granule membrane; Single-pass membrane protein Potential.
Miscellaneous	Present with 2930 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Endosome Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular aldehyde metabolic process Ref.6 Inferred by curator. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to mitochondrial outer membrane Ref.5 Inferred from direct assay. Source: SGD lipid particle Ref.2 Inferred from direct assay. Source: SGD
Molecular function	aldehyde dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: InterPro identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-27205,EBI-27205
PRP40	P33203	1	EBI-27205,EBI-701
SSM4	P40318	1	EBI-27205,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Putative fatty aldehyde dehydrogenase HFD1		PRO_0000056597
Regions
	Transmembrane	134 – 152	19	Potential
Sites
	Active site	236	1	By similarity
	Active site	273	1	By similarity
Amino acid modifications
	Modified residue	111	1	Phosphoserine Ref.7 Ref.8 Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q04458-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 6CAF4BFCF963AF88	FASTA	532	59,979
	10 20 30 40 50 60 MSNDGSKILN YTPVSKIDEI VEISRNFFFE KQLKLSHENN PRKKDLEFRQ LQLKKLYYAV 70 80 90 100 110 120 KDHEEELIDA MYKDFHRNKI ESVLNETTKL MNDILHLIEI LPKLIKPRRV SDSSPPFMFG 130 140 150 160 170 180 KTIVEKISRG SVLIIAPFNF PLLLAFAPLA AALAAGNTIV LKPSELTPHT AVVMENLLTT 190 200 210 220 230 240 AGFPDGLIQV VQGAIDETTR LLDCGKFDLI FYTGSPRVGS IVAEKAAKSL TPCVLELGGK 250 260 270 280 290 300 SPTFITENFK ASNIKIALKR IFFGAFGNSG QICVSPDYLL VHKSIYPKVI KECESVLNEF 310 320 330 340 350 360 YPSFDEQTDF TRMIHEPAYK KAVASINSTN GSKIVPSKIS INSDTEDLCL VPPTIVYNIG 370 380 390 400 410 420 WDDPLMKQEN FAPVLPIIEY EDLDETINKI IEEHDTPLVQ YIFSDSQTEI NRILTRLRSG 430 440 450 460 470 480 DCVVGDTVIH VGITDAPFGG IGTSGYGNYG GYYGFNTFSH ERTIFKQPYW NDFTLFMRYP 490 500 510 520 530 PNSAQKEKLV RFAMERKPWF DRNGNNKWGL RQYFSLSAAV ILISTIYAHC SS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae." Burri L., Vascotto K., Gentle I.E., Chan N.C., Beilharz T., Stapleton D.I., Ramage L., Lithgow T. FEBS J. 273:1507-1515(2006) [PubMed: 16689936] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[6]	"Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins." Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C. Mol. Biol. Cell 17:1436-1450(2006) [PubMed: 16407407] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111, MASS SPECTROMETRY.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49702 Genomic DNA. Translation: CAA89746.1.
PIR	S54571.
RefSeq	NP_013828.1.
3D structure databases
HSSP	HSSP built from PDB template 1AD3 based on UniProtKB P11883.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4437N.
IntAct	Q04458. 9 interactions.
Proteomic databases
PeptideAtlas	Q04458.
PRIDE	Q04458.
Genome annotation databases
Ensembl	YMR110C. Saccharomyces cerevisiae. [Contig view]
GeneID	855137.
GenomeReviews	Gene locus YMR110C in contig Z71257_GR.
KEGG	sce:YMR110C.
NMPDR	fig|4932.3.peg.4876.
Organism-specific databases
CYGD	YMR110c.
SGD	S000004716. HFD1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q04458.
OMA	Q04458. IYIERIP.
Gene expression databases
GermOnline	YMR110C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR012394. Aldehyde_DH_NAD(P). [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF15. ALDH. 1 hit.
Pfam	PF00171. Aldedh. 3 hits. [Graphical view]
PIRSF	PIRSF036492. ALDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. False negative. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978519.

Entry information

Entry name

HFD1_YEAST

Accession

Primary (citable) accession number: Q04458

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)