Q04457 (EST1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gut esterase 1
EC=3.1.1.1

Alternative name(s):
Non-specific carboxylesterase

Gene names

Name:	ges-1
ORF Names:	R12A1.4

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A carboxylic ester + H2O = an alcohol + a carboxylate.
Subcellular location	Endoplasmic reticulum lumen.
Tissue specificity	Expressed only in the intestine.
Developmental stage	Appears in mid-proliferation phase when the developing gut has four to eight cells.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell peroxisome Inferred from direct assay PubMed 18006689. Source: WormBase
Molecular_function	carboxylesterase activity Inferred from direct assay PubMed 2379823. Source: WormBase methyl indole-3-acetate esterase activity Inferred from electronic annotation. Source: EC methyl jasmonate esterase activity Inferred from electronic annotation. Source: EC methyl salicylate esterase activity Inferred from electronic annotation. Source: EC retinyl-palmitate esterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.1
	Chain	17 – 562	546	Gut esterase 1		PRO_0000008549
Regions
	Motif	559 – 562	4	Prevents secretion from ER
Sites
	Active site	198	1	Acyl-ester intermediate By similarity
	Active site	319	1	Charge relay system By similarity
	Active site	452	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	73	1	N-linked (GlcNAc...) Ref.3
	Disulfide bond	75 ↔ 93		By similarity
	Disulfide bond	250 ↔ 258		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04457 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 1366AA79ED84D96A	FASTA	562	63,722
	10 20 30 40 50 60 MRIFLVSVIL INACWAGPIV ETNYGKVEGI EYEGAEVFLA IPFAKPPVDD LRFEKPVAPD 70 80 90 100 110 120 PWEDVYPATQ YRNDCTPHYR LVAQFSSYSG EDCLTLNIIK PKKAEKLPVL FWIHGGGYEI 130 140 150 160 170 180 GSASQHGYEF FAKRYASQGV IVATVQYRLG FMGFFSEGTS DVQGNWGLFD QAAALEFVKS 190 200 210 220 230 240 NIENFGGDPN QITIWGYSAG AASVSQLTMS PYTRDSYSKA IIMSASSFVG WATGPNVVET 250 260 270 280 290 300 SKQLAEILGC PWPGAKECMK KKSLHEIFDA IEVQGWTTGT IDILRWSPVI DGDFMTKNPE 310 320 330 340 350 360 ELIKESPVKP TLIGMSNKEG SYFAALNMGR VIADFGLSPE DMPKVDEEFI SEIIGRKLLY 370 380 390 400 410 420 NNRYGENREK VWNDILDFYV KQGKPAEVKD LNGFYVDRYS ELLSDITFNV PILREITSRV 430 440 450 460 470 480 ERKTPVWTYR MDHYDKNIWK KHIPEQARGS PHANEYHYLF DMPVMAKIDM KKEPDSWIQN 490 500 510 520 530 540 DLIDMVISFA KTGVPQIEDV EWRPVSDPDD VNFLNIRSDG VSIEHGLFQE PLAFWNELRQ 550 560 REGFDLIDPT NSAMHSSNKD EL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gut esterase gene (ges-1) from the nematodes Caenorhabditis elegans and Caenorhabditis briggsae." Kennedy B.P., Aamodt E.J., Allen F.L., Chung M.A., Heschl M.F.P., McGhee J.D. J. Mol. Biol. 229:890-908(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-54.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins." Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T. Nat. Biotechnol. 21:667-672(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-73, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96145 Genomic DNA. Translation: AAA28057.1. FO081062 Genomic DNA. Translation: CCD68892.1.
PIR	S27800. T32061.
RefSeq	NP_503411.1. NM_071010.5.
UniGene	Cel.7142.
3D structure databases
ProteinModelPortal	Q04457.
SMR	Q04457. Positions 18-538.
ModBase	Search...
Protein family/group databases
MEROPS	S09.979.
Proteomic databases
PaxDb	Q04457.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	R12A1.4.1; R12A1.4.1; R12A1.4. R12A1.4.2; R12A1.4.2; R12A1.4.
GeneID	178633.
KEGG	cel:CELE_R12A1.4.
UCSC	R12A1.4.1. c. elegans.
Organism-specific databases
CTD	178633.
WormBase	R12A1.4; CE28763; WBGene00001578; ges-1.
Phylogenomic databases
eggNOG	COG2272.
GeneTree	ENSGT00680000100015.
InParanoid	Q04457.
KO	K01044.
OMA	AIPFAKP.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. [Graphical view]
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	901924.

Entry information

Entry name

EST1_CAEEL

Accession

Primary (citable) accession number: Q04457
Secondary accession number(s): O16702

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 29, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project