SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q04457

- EST1_CAEEL

UniProt

Q04457 - EST1_CAEEL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Gut esterase 1
Gene
ges-1, R12A1.4
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei198 – 1981Acyl-ester intermediate By similarity
Active sitei319 – 3191Charge relay system By similarity
Active sitei452 – 4521Charge relay system By similarity

GO - Molecular functioni

  1. carboxylic ester hydrolase activity Source: WormBase

GO - Biological processi

  1. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Protein family/group databases

MEROPSiS09.979.

Names & Taxonomyi

Protein namesi
Recommended name:
Gut esterase 1 (EC:3.1.1.1)
Alternative name(s):
Non-specific carboxylesterase
Gene namesi
Name:ges-1
ORF Names:R12A1.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiR12A1.4; CE28763; WBGene00001578; ges-1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  2. peroxisome Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 Publication
Add
BLAST
Chaini17 – 562546Gut esterase 1
PRO_0000008549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi73 – 731N-linked (GlcNAc...); atypical1 Publication
Disulfide bondi75 ↔ 93 By similarity
Disulfide bondi250 ↔ 258 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ04457.

Expressioni

Tissue specificityi

Expressed only in the intestine.

Developmental stagei

Appears in mid-proliferation phase when the developing gut has four to eight cells.

Structurei

3D structure databases

ProteinModelPortaliQ04457.
SMRiQ04457. Positions 18-538.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi559 – 5624Prevents secretion from ER

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2272.
GeneTreeiENSGT00680000100015.
InParanoidiQ04457.
KOiK01044.
OMAiAIPFAKP.
PhylomeDBiQ04457.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04457-1 [UniParc]FASTAAdd to Basket

« Hide

MRIFLVSVIL INACWAGPIV ETNYGKVEGI EYEGAEVFLA IPFAKPPVDD    50
LRFEKPVAPD PWEDVYPATQ YRNDCTPHYR LVAQFSSYSG EDCLTLNIIK 100
PKKAEKLPVL FWIHGGGYEI GSASQHGYEF FAKRYASQGV IVATVQYRLG 150
FMGFFSEGTS DVQGNWGLFD QAAALEFVKS NIENFGGDPN QITIWGYSAG 200
AASVSQLTMS PYTRDSYSKA IIMSASSFVG WATGPNVVET SKQLAEILGC 250
PWPGAKECMK KKSLHEIFDA IEVQGWTTGT IDILRWSPVI DGDFMTKNPE 300
ELIKESPVKP TLIGMSNKEG SYFAALNMGR VIADFGLSPE DMPKVDEEFI 350
SEIIGRKLLY NNRYGENREK VWNDILDFYV KQGKPAEVKD LNGFYVDRYS 400
ELLSDITFNV PILREITSRV ERKTPVWTYR MDHYDKNIWK KHIPEQARGS 450
PHANEYHYLF DMPVMAKIDM KKEPDSWIQN DLIDMVISFA KTGVPQIEDV 500
EWRPVSDPDD VNFLNIRSDG VSIEHGLFQE PLAFWNELRQ REGFDLIDPT 550
NSAMHSSNKD EL 562
Length:562
Mass (Da):63,722
Last modified:October 1, 1993 - v1
Checksum:i1366AA79ED84D96A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96145 Genomic DNA. Translation: AAA28057.1.
FO081062 Genomic DNA. Translation: CCD68892.1.
PIRiS27800.
T32061.
RefSeqiNP_503411.1. NM_071010.5.
UniGeneiCel.7142.

Genome annotation databases

EnsemblMetazoaiR12A1.4; R12A1.4; WBGene00001578.
GeneIDi178633.
KEGGicel:CELE_R12A1.4.
UCSCiR12A1.4.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96145 Genomic DNA. Translation: AAA28057.1 .
FO081062 Genomic DNA. Translation: CCD68892.1 .
PIRi S27800.
T32061.
RefSeqi NP_503411.1. NM_071010.5.
UniGenei Cel.7142.

3D structure databases

ProteinModelPortali Q04457.
SMRi Q04457. Positions 18-538.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S09.979.

Proteomic databases

PaxDbi Q04457.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai R12A1.4 ; R12A1.4 ; WBGene00001578 .
GeneIDi 178633.
KEGGi cel:CELE_R12A1.4.
UCSCi R12A1.4.1. c. elegans.

Organism-specific databases

CTDi 178633.
WormBasei R12A1.4 ; CE28763 ; WBGene00001578 ; ges-1.

Phylogenomic databases

eggNOGi COG2272.
GeneTreei ENSGT00680000100015.
InParanoidi Q04457.
KOi K01044.
OMAi AIPFAKP.
PhylomeDBi Q04457.

Miscellaneous databases

NextBioi 901924.
PROi Q04457.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view ]
Pfami PF00135. COesterase. 1 hit.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gut esterase gene (ges-1) from the nematodes Caenorhabditis elegans and Caenorhabditis briggsae."
    Kennedy B.P., Aamodt E.J., Allen F.L., Chung M.A., Heschl M.F.P., McGhee J.D.
    J. Mol. Biol. 229:890-908(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-54.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins."
    Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T.
    Nat. Biotechnol. 21:667-672(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-73, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiEST1_CAEEL
AccessioniPrimary (citable) accession number: Q04457
Secondary accession number(s): O16702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi