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Protein

Gut esterase 1

Gene

ges-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei198 – 1981Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei319 – 3191Charge relay systemBy similarity
Active sitei452 – 4521Charge relay systemBy similarity

GO - Molecular functioni

  1. carboxylic ester hydrolase activity Source: WormBase

GO - Biological processi

  1. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Names & Taxonomyi

Protein namesi
Recommended name:
Gut esterase 1 (EC:3.1.1.1)
Alternative name(s):
Non-specific carboxylesterase
Gene namesi
Name:ges-1
ORF Names:R12A1.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiR12A1.4; CE28763; WBGene00001578; ges-1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  2. extracellular space Source: GO_Central
  3. peroxisome Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Chaini17 – 562546Gut esterase 1PRO_0000008549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi73 – 731N-linked (GlcNAc...); atypical1 Publication
Disulfide bondi75 ↔ 93By similarity
Disulfide bondi250 ↔ 258By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ04457.

Expressioni

Tissue specificityi

Expressed only in the intestine.

Developmental stagei

Appears in mid-proliferation phase when the developing gut has four to eight cells.

Structurei

3D structure databases

ProteinModelPortaliQ04457.
SMRiQ04457. Positions 18-538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi559 – 5624Prevents secretion from ER

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2272.
GeneTreeiENSGT00680000100015.
InParanoidiQ04457.
KOiK01044.
OMAiRGSPHAN.
PhylomeDBiQ04457.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIFLVSVIL INACWAGPIV ETNYGKVEGI EYEGAEVFLA IPFAKPPVDD
60 70 80 90 100
LRFEKPVAPD PWEDVYPATQ YRNDCTPHYR LVAQFSSYSG EDCLTLNIIK
110 120 130 140 150
PKKAEKLPVL FWIHGGGYEI GSASQHGYEF FAKRYASQGV IVATVQYRLG
160 170 180 190 200
FMGFFSEGTS DVQGNWGLFD QAAALEFVKS NIENFGGDPN QITIWGYSAG
210 220 230 240 250
AASVSQLTMS PYTRDSYSKA IIMSASSFVG WATGPNVVET SKQLAEILGC
260 270 280 290 300
PWPGAKECMK KKSLHEIFDA IEVQGWTTGT IDILRWSPVI DGDFMTKNPE
310 320 330 340 350
ELIKESPVKP TLIGMSNKEG SYFAALNMGR VIADFGLSPE DMPKVDEEFI
360 370 380 390 400
SEIIGRKLLY NNRYGENREK VWNDILDFYV KQGKPAEVKD LNGFYVDRYS
410 420 430 440 450
ELLSDITFNV PILREITSRV ERKTPVWTYR MDHYDKNIWK KHIPEQARGS
460 470 480 490 500
PHANEYHYLF DMPVMAKIDM KKEPDSWIQN DLIDMVISFA KTGVPQIEDV
510 520 530 540 550
EWRPVSDPDD VNFLNIRSDG VSIEHGLFQE PLAFWNELRQ REGFDLIDPT
560
NSAMHSSNKD EL
Length:562
Mass (Da):63,722
Last modified:October 1, 1993 - v1
Checksum:i1366AA79ED84D96A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96145 Genomic DNA. Translation: AAA28057.1.
FO081062 Genomic DNA. Translation: CCD68892.1.
PIRiS27800.
T32061.
RefSeqiNP_503411.1. NM_071010.5.
UniGeneiCel.7142.

Genome annotation databases

EnsemblMetazoaiR12A1.4; R12A1.4; WBGene00001578.
GeneIDi178633.
KEGGicel:CELE_R12A1.4.
UCSCiR12A1.4.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96145 Genomic DNA. Translation: AAA28057.1.
FO081062 Genomic DNA. Translation: CCD68892.1.
PIRiS27800.
T32061.
RefSeqiNP_503411.1. NM_071010.5.
UniGeneiCel.7142.

3D structure databases

ProteinModelPortaliQ04457.
SMRiQ04457. Positions 18-538.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ04457.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR12A1.4; R12A1.4; WBGene00001578.
GeneIDi178633.
KEGGicel:CELE_R12A1.4.
UCSCiR12A1.4.1. c. elegans.

Organism-specific databases

CTDi178633.
WormBaseiR12A1.4; CE28763; WBGene00001578; ges-1.

Phylogenomic databases

eggNOGiCOG2272.
GeneTreeiENSGT00680000100015.
InParanoidiQ04457.
KOiK01044.
OMAiRGSPHAN.
PhylomeDBiQ04457.

Miscellaneous databases

NextBioi901924.
PROiQ04457.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gut esterase gene (ges-1) from the nematodes Caenorhabditis elegans and Caenorhabditis briggsae."
    Kennedy B.P., Aamodt E.J., Allen F.L., Chung M.A., Heschl M.F.P., McGhee J.D.
    J. Mol. Biol. 229:890-908(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-54.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins."
    Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T.
    Nat. Biotechnol. 21:667-672(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-73, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiEST1_CAEEL
AccessioniPrimary (citable) accession number: Q04457
Secondary accession number(s): O16702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 4, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.