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Q04457

- EST1_CAEEL

UniProt

Q04457 - EST1_CAEEL

Protein

Gut esterase 1

Gene

ges-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A carboxylic ester + H2O = an alcohol + a carboxylate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei198 – 1981Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei319 – 3191Charge relay systemBy similarity
    Active sitei452 – 4521Charge relay systemBy similarity

    GO - Molecular functioni

    1. carboxylic ester hydrolase activity Source: WormBase

    GO - Biological processi

    1. metabolic process Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Protein family/group databases

    MEROPSiS09.979.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gut esterase 1 (EC:3.1.1.1)
    Alternative name(s):
    Non-specific carboxylesterase
    Gene namesi
    Name:ges-1
    ORF Names:R12A1.4
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiR12A1.4; CE28763; WBGene00001578; ges-1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    2. peroxisome Source: WormBase

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 16161 PublicationAdd
    BLAST
    Chaini17 – 562546Gut esterase 1PRO_0000008549Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi73 – 731N-linked (GlcNAc...); atypical1 Publication
    Disulfide bondi75 ↔ 93By similarity
    Disulfide bondi250 ↔ 258By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ04457.

    Expressioni

    Tissue specificityi

    Expressed only in the intestine.

    Developmental stagei

    Appears in mid-proliferation phase when the developing gut has four to eight cells.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04457.
    SMRiQ04457. Positions 18-538.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi559 – 5624Prevents secretion from ER

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2272.
    GeneTreeiENSGT00680000100015.
    InParanoidiQ04457.
    KOiK01044.
    OMAiAIPFAKP.
    PhylomeDBiQ04457.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    [Graphical view]
    PfamiPF00135. COesterase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04457-1 [UniParc]FASTAAdd to Basket

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    MRIFLVSVIL INACWAGPIV ETNYGKVEGI EYEGAEVFLA IPFAKPPVDD    50
    LRFEKPVAPD PWEDVYPATQ YRNDCTPHYR LVAQFSSYSG EDCLTLNIIK 100
    PKKAEKLPVL FWIHGGGYEI GSASQHGYEF FAKRYASQGV IVATVQYRLG 150
    FMGFFSEGTS DVQGNWGLFD QAAALEFVKS NIENFGGDPN QITIWGYSAG 200
    AASVSQLTMS PYTRDSYSKA IIMSASSFVG WATGPNVVET SKQLAEILGC 250
    PWPGAKECMK KKSLHEIFDA IEVQGWTTGT IDILRWSPVI DGDFMTKNPE 300
    ELIKESPVKP TLIGMSNKEG SYFAALNMGR VIADFGLSPE DMPKVDEEFI 350
    SEIIGRKLLY NNRYGENREK VWNDILDFYV KQGKPAEVKD LNGFYVDRYS 400
    ELLSDITFNV PILREITSRV ERKTPVWTYR MDHYDKNIWK KHIPEQARGS 450
    PHANEYHYLF DMPVMAKIDM KKEPDSWIQN DLIDMVISFA KTGVPQIEDV 500
    EWRPVSDPDD VNFLNIRSDG VSIEHGLFQE PLAFWNELRQ REGFDLIDPT 550
    NSAMHSSNKD EL 562
    Length:562
    Mass (Da):63,722
    Last modified:October 1, 1993 - v1
    Checksum:i1366AA79ED84D96A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96145 Genomic DNA. Translation: AAA28057.1.
    FO081062 Genomic DNA. Translation: CCD68892.1.
    PIRiS27800.
    T32061.
    RefSeqiNP_503411.1. NM_071010.5.
    UniGeneiCel.7142.

    Genome annotation databases

    EnsemblMetazoaiR12A1.4; R12A1.4; WBGene00001578.
    GeneIDi178633.
    KEGGicel:CELE_R12A1.4.
    UCSCiR12A1.4.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96145 Genomic DNA. Translation: AAA28057.1 .
    FO081062 Genomic DNA. Translation: CCD68892.1 .
    PIRi S27800.
    T32061.
    RefSeqi NP_503411.1. NM_071010.5.
    UniGenei Cel.7142.

    3D structure databases

    ProteinModelPortali Q04457.
    SMRi Q04457. Positions 18-538.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S09.979.

    Proteomic databases

    PaxDbi Q04457.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai R12A1.4 ; R12A1.4 ; WBGene00001578 .
    GeneIDi 178633.
    KEGGi cel:CELE_R12A1.4.
    UCSCi R12A1.4.1. c. elegans.

    Organism-specific databases

    CTDi 178633.
    WormBasei R12A1.4 ; CE28763 ; WBGene00001578 ; ges-1.

    Phylogenomic databases

    eggNOGi COG2272.
    GeneTreei ENSGT00680000100015.
    InParanoidi Q04457.
    KOi K01044.
    OMAi AIPFAKP.
    PhylomeDBi Q04457.

    Miscellaneous databases

    NextBioi 901924.
    PROi Q04457.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    [Graphical view ]
    Pfami PF00135. COesterase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gut esterase gene (ges-1) from the nematodes Caenorhabditis elegans and Caenorhabditis briggsae."
      Kennedy B.P., Aamodt E.J., Allen F.L., Chung M.A., Heschl M.F.P., McGhee J.D.
      J. Mol. Biol. 229:890-908(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-54.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins."
      Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T.
      Nat. Biotechnol. 21:667-672(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-73, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiEST1_CAEEL
    AccessioniPrimary (citable) accession number: Q04457
    Secondary accession number(s): O16702
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3