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Q04457 (EST1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gut esterase 1

EC=3.1.1.1
Alternative name(s):
Non-specific carboxylesterase
Gene names
Name:ges-1
ORF Names:R12A1.4
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A carboxylic ester + H2O = an alcohol + a carboxylate.

Subcellular location

Endoplasmic reticulum lumen.

Tissue specificity

Expressed only in the intestine.

Developmental stage

Appears in mid-proliferation phase when the developing gut has four to eight cells.

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Ref.1
Chain17 – 562546Gut esterase 1
PRO_0000008549

Regions

Motif559 – 5624Prevents secretion from ER

Sites

Active site1981Acyl-ester intermediate By similarity
Active site3191Charge relay system By similarity
Active site4521Charge relay system By similarity

Amino acid modifications

Glycosylation731N-linked (GlcNAc...); atypical Ref.3
Disulfide bond75 ↔ 93 By similarity
Disulfide bond250 ↔ 258 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q04457 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 1366AA79ED84D96A

FASTA56263,722
        10         20         30         40         50         60 
MRIFLVSVIL INACWAGPIV ETNYGKVEGI EYEGAEVFLA IPFAKPPVDD LRFEKPVAPD 

        70         80         90        100        110        120 
PWEDVYPATQ YRNDCTPHYR LVAQFSSYSG EDCLTLNIIK PKKAEKLPVL FWIHGGGYEI 

       130        140        150        160        170        180 
GSASQHGYEF FAKRYASQGV IVATVQYRLG FMGFFSEGTS DVQGNWGLFD QAAALEFVKS 

       190        200        210        220        230        240 
NIENFGGDPN QITIWGYSAG AASVSQLTMS PYTRDSYSKA IIMSASSFVG WATGPNVVET 

       250        260        270        280        290        300 
SKQLAEILGC PWPGAKECMK KKSLHEIFDA IEVQGWTTGT IDILRWSPVI DGDFMTKNPE 

       310        320        330        340        350        360 
ELIKESPVKP TLIGMSNKEG SYFAALNMGR VIADFGLSPE DMPKVDEEFI SEIIGRKLLY 

       370        380        390        400        410        420 
NNRYGENREK VWNDILDFYV KQGKPAEVKD LNGFYVDRYS ELLSDITFNV PILREITSRV 

       430        440        450        460        470        480 
ERKTPVWTYR MDHYDKNIWK KHIPEQARGS PHANEYHYLF DMPVMAKIDM KKEPDSWIQN 

       490        500        510        520        530        540 
DLIDMVISFA KTGVPQIEDV EWRPVSDPDD VNFLNIRSDG VSIEHGLFQE PLAFWNELRQ 

       550        560 
REGFDLIDPT NSAMHSSNKD EL 

« Hide

References

« Hide 'large scale' references
[1]"The gut esterase gene (ges-1) from the nematodes Caenorhabditis elegans and Caenorhabditis briggsae."
Kennedy B.P., Aamodt E.J., Allen F.L., Chung M.A., Heschl M.F.P., McGhee J.D.
J. Mol. Biol. 229:890-908(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-54.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins."
Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T.
Nat. Biotechnol. 21:667-672(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-73, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96145 Genomic DNA. Translation: AAA28057.1.
FO081062 Genomic DNA. Translation: CCD68892.1.
PIRS27800.
T32061.
RefSeqNP_503411.1. NM_071010.5.
UniGeneCel.7142.

3D structure databases

ProteinModelPortalQ04457.
SMRQ04457. Positions 18-538.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS09.979.

Proteomic databases

PaxDbQ04457.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaR12A1.4; R12A1.4; WBGene00001578.
GeneID178633.
KEGGcel:CELE_R12A1.4.
UCSCR12A1.4.1. c. elegans.

Organism-specific databases

CTD178633.
WormBaseR12A1.4; CE28763; WBGene00001578; ges-1.

Phylogenomic databases

eggNOGCOG2272.
GeneTreeENSGT00680000100015.
InParanoidQ04457.
KOK01044.
OMAAIPFAKP.
PhylomeDBQ04457.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio901924.
PROQ04457.

Entry information

Entry nameEST1_CAEEL
AccessionPrimary (citable) accession number: Q04457
Secondary accession number(s): O16702
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase