Q04456 (EST1_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gut esterase 1
EC=3.1.1.1

Alternative name(s):
Non-specific carboxylesterase

Gene names

Name:	ges-1
ORF Names:	CBG06418

Organism

Caenorhabditis briggsae [Reference proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A carboxylic ester + H2O = an alcohol + a carboxylate.
Subcellular location	Endoplasmic reticulum lumen.
Tissue specificity	Expressed only in the intestine.
Developmental stage	Appears in mid-proliferation phase when the developing gut has four to eight cells.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW methyl indole-3-acetate esterase activity Inferred from electronic annotation. Source: EC methyl jasmonate esterase activity Inferred from electronic annotation. Source: EC methyl salicylate esterase activity Inferred from electronic annotation. Source: EC retinyl-palmitate esterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16
	Chain	17 – 562	546	Gut esterase 1		PRO_0000008550
Regions
	Motif	559 – 562	4	Prevents secretion from ER
Sites
	Active site	199	1	Acyl-ester intermediate By similarity
	Active site	320	1	Charge relay system By similarity
	Active site	451	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	75 ↔ 93		By similarity
	Disulfide bond	251 ↔ 259		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04456 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 7FA312F3B2587E53	FASTA	562	63,819
	10 20 30 40 50 60 MRVLLASLLI FGACWAGPVV NTNYGKVEGF EYGAAEVFLA IPFAKPPVDN LRFEKPEAPE 70 80 90 100 110 120 PWEDVYQATQ FRNDCTPHYR LVAQFSSYSG EDCLTLNVIK PKTIEKKLPV LFWVHGGGYE 130 140 150 160 170 180 IGSGSQHGYE FFADRYTSQG VIVVTIQYRL GFMGFFSEGT SDAPGNYGLF DQAAALRFVK 190 200 210 220 230 240 ENIGNFGGDP DDITIWGYSA GAASVSQLTM SPYTHDLYSK AIIMSASSFV GWATGPNVID 250 260 270 280 290 300 TSKQLAEILG CPWPGAKECM KKKTLHEIFD AVETQGWTTG TIDILRWSPV IDGDYLPKNP 310 320 330 340 350 360 ENLINDAPIK PTLIGMSNKE GSYFATMNMG RVIADFGLSP EEIPKVDEDF ISEIIDRKLL 370 380 390 400 410 420 YNNRYGENRQ KVWDQILDYY TKQGKPERDL NGFYVDRYAE LLSDITFNVP ILREITARVE 430 440 450 460 470 480 RKTPVWTYRF DHYNEQIWKK YIPEQAKGSP HANEYHYLFN MPVMAQIDFK KEPESWLQRD 490 500 510 520 530 540 LIDMVVSFAK TGVPHIQDVE WRPVSDPDDV NFLNFQSSGV SVKHGLFQEP LDFWNNLRER 550 560 EGFDLVDPAY SKTTSNSEKD EL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gut esterase gene (ges-1) from the nematodes Caenorhabditis elegans and Caenorhabditis briggsae." Kennedy B.P., Aamodt E.J., Allen F.L., Chung M.A., Heschl M.F.P., McGhee J.D. J. Mol. Biol. 229:890-908(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96144 Genomic DNA. Translation: AAA28056.1. HE601320 Genomic DNA. Translation: CAP26727.1.
PIR	S27782.
RefSeq	XP_002647363.1. XM_002647317.1.
3D structure databases
ProteinModelPortal	Q04456.
ModBase	Search...
Protein family/group databases
MEROPS	S09.979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	CBG06418; CBG06418; CBG06418.
GeneID	8589362.
KEGG	cbr:CBG06418.
Organism-specific databases
CTD	8589362.
WormBase	CBG06418; CBP15596; WBGene00028697; Cbr-ges-1.
Phylogenomic databases
eggNOG	COG2272.
OMA	AIPFAKP.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. [Graphical view]
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EST1_CAEBR

Accession

Primary (citable) accession number: Q04456
Secondary accession number(s): A8X267

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project