Q04448 (MTDC_DROME) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 96.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial Short name=DNMDMC | ||||
| Gene names |
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| Organism | Drosophila melanogaster (Fruit fly) | ||||
| Taxonomic identifier | 7227 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 309 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | May play a role in spermatogenesis. |
| Catalytic activity | 5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH. 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. |
| Cofactor | Magnesium. |
| Subunit structure | Homodimer. |
| Subcellular location | |
| Developmental stage | Expressed in developing tissues and at high levels in adult tissues. Isoform A shows male-specific expression. Ref.1 |
| Miscellaneous | This NAD-dependent bifunctional enzyme has very different kinetic properties than the larger NADP-dependent trifunctional enzyme and is unique in that it requires formation of an enzyme-magnesium complex to allow binding of NAD. |
| Sequence similarities | Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform B (identifier: Q04448-1) Also known as: Hedengren-A; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform A (identifier: Q04448-2) Also known as: Hedengren-B; The sequence of this isoform differs from the canonical sequence as follows: 1-6: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||
Molecule processing | ||||||||
|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Mitochondrion Potential | ||||||
| Chain | ? – 309 | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial | PRO_0000034051 | |||||
Natural variations | ||||||||
| Alternative sequence | 1 – 6 | 6 | Missing in isoform A. | VSP_016458 | ||||
Experimental info | ||||||||
| Sequence conflict | 1 – 4 | 4 | MRFT → MATVMGPTPPGTGVMWPAIW RTSSKAIGIRQSVQFEFSTR KISQKPQKEVTI Ref.1 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The isolation and characterization of a Drosophila gene encoding a putative NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase." Price B.D., Laughon A. Biochim. Biophys. Acta 1173:94-98(1993) [PubMed: 8485162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. |
| [2] | "Relish, a central factor in the control of humoral, but not cellular immunity in Drosophila." Hedengren M., Asling B., Dushay M.S., Ando I., Ekengren S., Wihlborg M., Hultmark D. Mol. Cell 4:827-837(1999) [PubMed: 10619029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. |
| [3] | "The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. Venter J.C.Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley. |
| [4] | "Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. Lewis S.E.Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley. |
| [5] | "A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Head. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | L07958 mRNA. Translation: AAB41352.1. AF186073 Genomic DNA. Translation: AAF07929.1. AF186073 Genomic DNA. Translation: AAF07930.1. AE014297 Genomic DNA. Translation: AAF54332.1. AE014297 Genomic DNA. Translation: AAN13408.1. AY047498 mRNA. Translation: AAK77230.1. |
| PIR | S32562. |
| RefSeq | NP_476929.1. NM_057581.4. NP_476930.1. NM_057582.4. |
| UniGene | Dm.33471. |
3D structure databases | |
| ProteinModelPortal | Q04448. |
| SMR | Q04448. Positions 7-308. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q04448. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblMetazoa | FBtr0081996; FBpp0081476; FBgn0010222. |
| GeneID | 47895. |
| KEGG | dme:Dmel_CG18466. |
Organism-specific databases | |
| CTD | 47895. |
| FlyBase | FBgn0010222. Nmdmc. |
Phylogenomic databases | |
| eggNOG | inNOG04050. |
| GeneTree | EMGT00050000016869. |
| InParanoid | Q04448. |
| OMA | YTPPKEL. |
| OrthoDB | EOG45DV53. |
| PhylomeDB | Q04448. |
Gene expression databases | |
| Bgee | Q04448. |
| GermOnline | CG18466. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. IPR020630. THF_DH/CycHdrlase_cat_dom. IPR020867. THF_DH/CycHdrlase_CS. IPR020631. THF_DH/CycHdrlase_NAD-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K13403. |
| Pfam | PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view] |
| PRINTS | PR00085. THFDHDRGNASE. |
| PROSITE | PS00766. THF_DHG_CYH_1. 1 hit. PS00767. THF_DHG_CYH_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 839208. |
Entry information
| Entry name | MTDC_DROME | ||||||||
| Accession | Primary (citable) accession number: Q04448 Secondary accession number(s): Q7KSU1, Q9U6H6, Q9V3H4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| SIMILARITY comments Index of protein domains and families |

Clusters with