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Protein

Creatine kinase B-type

Gene

Ckb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei130ATPPROSITE-ProRule annotation1
Binding sitei132ATPPROSITE-ProRule annotation1
Binding sitei191ATPPROSITE-ProRule annotation1
Binding sitei232SubstrateBy similarity1
Binding sitei236ATPPROSITE-ProRule annotation1
Binding sitei285SubstrateBy similarity1
Binding sitei292ATPPROSITE-ProRule annotation1
Binding sitei320ATPPROSITE-ProRule annotation1
Binding sitei335ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 132ATPPROSITE-ProRule annotation5
Nucleotide bindingi320 – 325ATPPROSITE-ProRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-71288 Creatine metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase B-type (EC:2.7.3.2)
Alternative name(s):
B-CK
Creatine kinase B chain
Creatine phosphokinase B-type
Short name:
CPK-B
Gene namesi
Name:Ckb
Synonyms:Ckbb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:88407 Ckb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002119671 – 381Creatine kinase B-typeAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei35PhosphothreonineCombined sources1
Modified residuei125PhosphotyrosineCombined sources1
Modified residuei199PhosphoserineCombined sources1
Modified residuei269Nitrated tyrosineCombined sources1
Modified residuei309PhosphoserineBy similarity1
Modified residuei322PhosphothreonineCombined sources1

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

EPDiQ04447
MaxQBiQ04447
PaxDbiQ04447
PeptideAtlasiQ04447
PRIDEiQ04447

2D gel databases

REPRODUCTION-2DPAGEiQ04447
UCD-2DPAGEiQ04447

PTM databases

iPTMnetiQ04447
PhosphoSitePlusiQ04447
SwissPalmiQ04447

Expressioni

Gene expression databases

BgeeiENSMUSG00000001270
CleanExiMM_CKB
GenevisibleiQ04447 MM

Interactioni

Subunit structurei

Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198725, 5 interactors
CORUMiQ04447
IntActiQ04447, 9 interactors
MINTiQ04447
STRINGi10090.ENSMUSP00000001304

Structurei

3D structure databases

ProteinModelPortaliQ04447
SMRiQ04447
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 98Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST88
Domaini125 – 367Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3581 Eukaryota
COG3869 LUCA
GeneTreeiENSGT00550000074561
HOGENOMiHOG000232165
HOVERGENiHBG001339
InParanoidiQ04447
KOiK00933
OMAiRGHEFMW
OrthoDBiEOG091G0HZ0
PhylomeDBiQ04447
TreeFamiTF314214

Family and domain databases

Gene3Di1.10.135.10, 1 hit
InterProiView protein in InterPro
IPR000749 ATP-guanido_PTrfase
IPR022415 ATP-guanido_PTrfase_AS
IPR022414 ATP-guanido_PTrfase_cat
IPR022413 ATP-guanido_PTrfase_N
IPR036802 ATP-guanido_PTrfase_N_sf
IPR014746 Gln_synth/guanido_kin_cat_dom
PANTHERiPTHR11547 PTHR11547, 1 hit
PfamiView protein in Pfam
PF00217 ATP-gua_Ptrans, 1 hit
PF02807 ATP-gua_PtransN, 1 hit
SUPFAMiSSF48034 SSF48034, 1 hit
SSF55931 SSF55931, 1 hit
PROSITEiView protein in PROSITE
PS00112 PHOSPHAGEN_KINASE, 1 hit
PS51510 PHOSPHAGEN_KINASE_C, 1 hit
PS51509 PHOSPHAGEN_KINASE_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q04447-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFSNSHNTQ KLRFPAEDEF PDLSSHNNHM AKVLTPELYA ELRAKCTPSG
60 70 80 90 100
FTLDDAIQTG VDNPGHPYIM TVGAVAGDEE SYDVFKDLFD PIIEERHGGY
110 120 130 140 150
QPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
160 170 180 190 200
RRAIEKLAVE ALSSLDGDLS GRYYALKSMT EAEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM QKGGNMKEVF
260 270 280 290 300
TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
310 320 330 340 350
HLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
360 370 380
QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K
Length:381
Mass (Da):42,713
Last modified:October 1, 1993 - v1
Checksum:iD901C5653054A490
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143P → H in BAE28690 (PubMed:16141072).Curated1
Sequence conflicti217I → M in BAE39162 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74149 Genomic DNA Translation: AAA37462.1
L09069 Genomic DNA Translation: AAA37455.1
AK002467 mRNA Translation: BAB22121.1
AK014299 mRNA Translation: BAB29254.1
AK148885 mRNA Translation: BAE28690.1
AK152388 mRNA Translation: BAE31176.1
AK153484 mRNA Translation: BAE32032.1
AK166980 mRNA Translation: BAE39162.1
AK161990 mRNA Translation: BAE36669.1
AK167034 mRNA Translation: BAE39205.1
BC015271 mRNA Translation: AAH15271.1
BC106109 mRNA Translation: AAI06110.2
X04591 mRNA Translation: CAA28259.1
CCDSiCCDS26183.1
PIRiA42078
RefSeqiNP_067248.1, NM_021273.4
UniGeneiMm.16831

Genome annotation databases

EnsembliENSMUST00000001304; ENSMUSP00000001304; ENSMUSG00000001270
GeneIDi12709
KEGGimmu:12709
UCSCiuc007pdn.2 mouse

Similar proteinsi

Entry informationi

Entry nameiKCRB_MOUSE
AccessioniPrimary (citable) accession number: Q04447
Secondary accession number(s): Q3KQP4
, Q3TKI3, Q3U5P5, Q3UF71, Q9CXK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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