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Protein

Creatine kinase B-type

Gene

Ckb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei130ATPPROSITE-ProRule annotation1
Binding sitei132ATPPROSITE-ProRule annotation1
Binding sitei191ATPPROSITE-ProRule annotation1
Binding sitei232SubstrateBy similarity1
Binding sitei236ATPPROSITE-ProRule annotation1
Binding sitei285SubstrateBy similarity1
Binding sitei292ATPPROSITE-ProRule annotation1
Binding sitei320ATPPROSITE-ProRule annotation1
Binding sitei335ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 132ATPPROSITE-ProRule annotation5
Nucleotide bindingi320 – 325ATPPROSITE-ProRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-71288. Creatine metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase B-type (EC:2.7.3.2)
Alternative name(s):
B-CK
Creatine kinase B chain
Gene namesi
Name:Ckb
Synonyms:Ckbb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:88407. Ckb.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002119672 – 381Creatine kinase B-typeAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei35PhosphothreonineCombined sources1
Modified residuei125PhosphotyrosineCombined sources1
Modified residuei199PhosphoserineCombined sources1
Modified residuei269Nitrated tyrosineCombined sources1
Modified residuei309PhosphoserineBy similarity1
Modified residuei322PhosphothreonineCombined sources1

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

EPDiQ04447.
MaxQBiQ04447.
PaxDbiQ04447.
PeptideAtlasiQ04447.
PRIDEiQ04447.

2D gel databases

REPRODUCTION-2DPAGEQ04447.
UCD-2DPAGEQ04447.

PTM databases

iPTMnetiQ04447.
PhosphoSitePlusiQ04447.
SwissPalmiQ04447.

Expressioni

Gene expression databases

BgeeiENSMUSG00000001270.
CleanExiMM_CKB.
GenevisibleiQ04447. MM.

Interactioni

Subunit structurei

Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198725. 4 interactors.
IntActiQ04447. 9 interactors.
MINTiMINT-4099724.
STRINGi10090.ENSMUSP00000001304.

Structurei

3D structure databases

ProteinModelPortaliQ04447.
SMRiQ04447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 98Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST88
Domaini125 – 367Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiQ04447.
KOiK00933.
OMAiRGHEFMW.
OrthoDBiEOG091G0HZ0.
PhylomeDBiQ04447.
TreeFamiTF314214.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04447-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFSNSHNTQ KLRFPAEDEF PDLSSHNNHM AKVLTPELYA ELRAKCTPSG
60 70 80 90 100
FTLDDAIQTG VDNPGHPYIM TVGAVAGDEE SYDVFKDLFD PIIEERHGGY
110 120 130 140 150
QPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
160 170 180 190 200
RRAIEKLAVE ALSSLDGDLS GRYYALKSMT EAEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM QKGGNMKEVF
260 270 280 290 300
TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
310 320 330 340 350
HLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
360 370 380
QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K
Length:381
Mass (Da):42,713
Last modified:October 1, 1993 - v1
Checksum:iD901C5653054A490
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143P → H in BAE28690 (PubMed:16141072).Curated1
Sequence conflicti217I → M in BAE39162 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74149 Genomic DNA. Translation: AAA37462.1.
L09069 Genomic DNA. Translation: AAA37455.1.
AK002467 mRNA. Translation: BAB22121.1.
AK014299 mRNA. Translation: BAB29254.1.
AK148885 mRNA. Translation: BAE28690.1.
AK152388 mRNA. Translation: BAE31176.1.
AK153484 mRNA. Translation: BAE32032.1.
AK166980 mRNA. Translation: BAE39162.1.
AK161990 mRNA. Translation: BAE36669.1.
AK167034 mRNA. Translation: BAE39205.1.
BC015271 mRNA. Translation: AAH15271.1.
BC106109 mRNA. Translation: AAI06110.2.
X04591 mRNA. Translation: CAA28259.1.
CCDSiCCDS26183.1.
PIRiA42078.
RefSeqiNP_067248.1. NM_021273.4.
UniGeneiMm.16831.

Genome annotation databases

EnsembliENSMUST00000001304; ENSMUSP00000001304; ENSMUSG00000001270.
GeneIDi12709.
KEGGimmu:12709.
UCSCiuc007pdn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74149 Genomic DNA. Translation: AAA37462.1.
L09069 Genomic DNA. Translation: AAA37455.1.
AK002467 mRNA. Translation: BAB22121.1.
AK014299 mRNA. Translation: BAB29254.1.
AK148885 mRNA. Translation: BAE28690.1.
AK152388 mRNA. Translation: BAE31176.1.
AK153484 mRNA. Translation: BAE32032.1.
AK166980 mRNA. Translation: BAE39162.1.
AK161990 mRNA. Translation: BAE36669.1.
AK167034 mRNA. Translation: BAE39205.1.
BC015271 mRNA. Translation: AAH15271.1.
BC106109 mRNA. Translation: AAI06110.2.
X04591 mRNA. Translation: CAA28259.1.
CCDSiCCDS26183.1.
PIRiA42078.
RefSeqiNP_067248.1. NM_021273.4.
UniGeneiMm.16831.

3D structure databases

ProteinModelPortaliQ04447.
SMRiQ04447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198725. 4 interactors.
IntActiQ04447. 9 interactors.
MINTiMINT-4099724.
STRINGi10090.ENSMUSP00000001304.

PTM databases

iPTMnetiQ04447.
PhosphoSitePlusiQ04447.
SwissPalmiQ04447.

2D gel databases

REPRODUCTION-2DPAGEQ04447.
UCD-2DPAGEQ04447.

Proteomic databases

EPDiQ04447.
MaxQBiQ04447.
PaxDbiQ04447.
PeptideAtlasiQ04447.
PRIDEiQ04447.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001304; ENSMUSP00000001304; ENSMUSG00000001270.
GeneIDi12709.
KEGGimmu:12709.
UCSCiuc007pdn.2. mouse.

Organism-specific databases

CTDi1152.
MGIiMGI:88407. Ckb.

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiQ04447.
KOiK00933.
OMAiRGHEFMW.
OrthoDBiEOG091G0HZ0.
PhylomeDBiQ04447.
TreeFamiTF314214.

Enzyme and pathway databases

ReactomeiR-MMU-71288. Creatine metabolism.

Miscellaneous databases

PROiQ04447.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001270.
CleanExiMM_CKB.
GenevisibleiQ04447. MM.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCRB_MOUSE
AccessioniPrimary (citable) accession number: Q04447
Secondary accession number(s): Q3KQP4
, Q3TKI3, Q3U5P5, Q3UF71, Q9CXK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.