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Protein

Cytochrome c oxidase subunit 2

Gene

ctaC

Organism
Bacillus pseudofirmus (strain OF4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B).

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi175 – 1751Copper ACurated
Metal bindingi210 – 2101Copper ACurated
Metal bindingi214 – 2141Copper ACurated
Metal bindingi218 – 2181Copper ACurated
Binding sitei264 – 2641Heme (covalent)Curated
Binding sitei267 – 2671Heme (covalent)Curated
Metal bindingi268 – 2681Iron (heme axial ligand)Curated
Metal bindingi317 – 3171Iron (heme axial ligand)Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBPSE398511:GJI9-2594-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 2 (EC:1.9.3.1)
Alternative name(s):
Cytochrome aa3 subunit 2
Cytochrome c oxidase polypeptide II
Oxidase aa(3) subunit 2
Gene namesi
Name:ctaC
Ordered Locus Names:BpOF4_00915
OrganismiBacillus pseudofirmus (strain OF4)
Taxonomic identifieri398511 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001544 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 5028ExtracellularSequence AnalysisAdd
BLAST
Transmembranei51 – 6919HelicalSequence AnalysisAdd
BLAST
Topological domaini70 – 8920CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei90 – 10819HelicalSequence AnalysisAdd
BLAST
Topological domaini109 – 342234ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222PROSITE-ProRule annotation1 PublicationAdd
BLAST
Chaini23 – 342320Cytochrome c oxidase subunit 2PRO_0000006049Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi23 – 231N-palmitoyl cysteinePROSITE-ProRule annotation
Lipidationi23 – 231S-diacylglycerol cysteinePROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Protein-protein interaction databases

STRINGi398511.BpOF4_00915.

Structurei

3D structure databases

ProteinModelPortaliQ04441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini250 – 34293Cytochrome cPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 249227Cytochrome c oxidase subunit IIAdd
BLAST

Sequence similaritiesi

Contains 1 cytochrome c domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000268576.
KOiK02275.

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
1.10.760.10. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR009056. Cyt_c-like_dom.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
PF02790. COX2_TM. 1 hit.
PF00034. Cytochrom_C. 1 hit.
[Graphical view]
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsiTIGR02866. CoxB. 1 hit.
PROSITEiPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
PS51007. CYTC. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLWKTASRF LPLSFLTLFL TGCLGEENLT ALDPKGPQAQ WIYDNMILSI
60 70 80 90 100
IVMALVSIVV FAIFFIILAK YRRKPGDDEI PKQVHGNTAL EITWTVIPII
110 120 130 140 150
LLVILAVPTI TGTFMFADKD PDPEVGDNTV YIKVTGHQFW WQFDYENEGF
160 170 180 190 200
TAGQDVYIPV GEKVIFELHA QDVLHSFWVP ALGGKIDTVP GITNHMWLEA
210 220 230 240 250
DEPGVFKGKC AELCGPSHAL MDFKLIALER DEYDAWVEGM SAEVEEPTET
260 270 280 290 300
LANQGRQVFE ENSCIGCHAV GGTGTAAGPA FTNFGEREVI AGYLENNDEN
310 320 330 340
LEAWIRDPQS LKQGNVMPAY PDMSEEDMEA LIAYLRSLKV ME
Length:342
Mass (Da):38,142
Last modified:February 1, 1994 - v1
Checksum:iF777C7D1FE44A429
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94110 Genomic DNA. Translation: AAA22364.1.
CP001878 Genomic DNA. Translation: ADC48252.1.
RefSeqiWP_012959534.1. NC_013791.2.
YP_003425144.1. NC_013791.2.

Genome annotation databases

EnsemblBacteriaiADC48252; ADC48252; BpOF4_00915.
KEGGibpf:BpOF4_00915.
PATRICi31942832. VBIBacPse80461_0178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94110 Genomic DNA. Translation: AAA22364.1.
CP001878 Genomic DNA. Translation: ADC48252.1.
RefSeqiWP_012959534.1. NC_013791.2.
YP_003425144.1. NC_013791.2.

3D structure databases

ProteinModelPortaliQ04441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi398511.BpOF4_00915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADC48252; ADC48252; BpOF4_00915.
KEGGibpf:BpOF4_00915.
PATRICi31942832. VBIBacPse80461_0178.

Phylogenomic databases

HOGENOMiHOG000268576.
KOiK02275.

Enzyme and pathway databases

BioCyciBPSE398511:GJI9-2594-MONOMER.

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
1.10.760.10. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR009056. Cyt_c-like_dom.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
PF02790. COX2_TM. 1 hit.
PF00034. Cytochrom_C. 1 hit.
[Graphical view]
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsiTIGR02866. CoxB. 1 hit.
PROSITEiPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
PS51007. CYTC. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the cta operon from alkaliphilic Bacillus firmus OF4 and characterization of the pH-regulated cytochrome caa3 oxidase it encodes."
    Quirk P.G., Hicks D.B., Krulwich T.A.
    J. Biol. Chem. 268:678-685(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-48.
  2. "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4."
    Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J., Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B., Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G.
    , Xue Y., Ma Y., Hu F.Z., Krulwich T.A.
    Environ. Microbiol. 13:3289-3309(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OF4.

Entry informationi

Entry nameiCOX2_BACPE
AccessioniPrimary (citable) accession number: Q04441
Secondary accession number(s): D3FU50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 24, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.