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Q04440

- COX1_BACPE

UniProt

Q04440 - COX1_BACPE

Protein

Cytochrome c oxidase subunit 1

Gene

ctaD

Organism
Bacillus pseudofirmus (strain OF4)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 3 (15 Jun 2010)
      Previous versions | rss
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    Functioni

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi69 – 691Iron (heme A axial ligand)Curated
    Metal bindingi246 – 2461Copper BCurated
    Metal bindingi250 – 2501Copper BCurated
    Metal bindingi295 – 2951Copper BCurated
    Metal bindingi296 – 2961Copper BCurated
    Metal bindingi381 – 3811Iron (heme A3 axial ligand)Curated
    Metal bindingi383 – 3831Iron (heme A axial ligand)Curated

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. cytochrome-c oxidase activity Source: UniProtKB-EC
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro

    GO - Biological processi

    1. aerobic respiration Source: InterPro
    2. electron transport chain Source: InterPro
    3. oxidative phosphorylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciBPSE398511:GJI9-2593-MONOMER.
    UniPathwayiUPA00705.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
    Alternative name(s):
    Cytochrome aa3 subunit 1
    Cytochrome c oxidase polypeptide I
    Gene namesi
    Name:ctaD
    Ordered Locus Names:BpOF4_00910
    OrganismiBacillus pseudofirmus (strain OF4)
    Taxonomic identifieri398511 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001544: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell
    3. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 625624Cytochrome c oxidase subunit 1PRO_0000183434Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki246 ↔ 2501'-histidyl-3'-tyrosine (His-Tyr)By similarity

    Expressioni

    Inductioni

    Elevated expression at high pH.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04440.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei23 – 4321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei72 – 9221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei99 – 11921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei151 – 17121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei195 – 21521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei240 – 26021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei272 – 29221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei309 – 32921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei343 – 36321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei382 – 40221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei417 – 43721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei460 – 48021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei551 – 57121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei577 – 59721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000085275.
    KOiK02274.

    Family and domain databases

    Gene3Di1.20.210.10. 1 hit.
    InterProiIPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    IPR014241. Cyt_c_oxidase_su1_bac.
    [Graphical view]
    PANTHERiPTHR10422. PTHR10422. 1 hit.
    PfamiPF00115. COX1. 1 hit.
    [Graphical view]
    PRINTSiPR01165. CYCOXIDASEI.
    SUPFAMiSSF81442. SSF81442. 1 hit.
    TIGRFAMsiTIGR02891. CtaD_CoxA. 1 hit.
    PROSITEiPS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q04440-1 [UniParc]FASTAAdd to Basket

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    MATQKQEKSV IWDWLTTVDH KKIAIMYLIA GTLFFVKAGV MALFMRIQLM    50
    YPEMNFLSGQ TFNEFITMHG TIMLFLAATP LLFAFMNYVI PLQIGARDVA 100
    FPFVNALGFW IFFFGGLLLS LSWFFGGGPD AGWTAYVPLS SRDYGGLGID 150
    FYVLGLQVSG IGTLISAINF LVTIVNMRAP GMTMMRLPLF VWTSFISSTL 200
    ILFAFTPLAA GLALLMLDRL FEAQYFIPSM GGNVVLWQHI FWIFGHPEVY 250
    ILVLPAFGII SEVIPAFSRK RLFGYTAMVF ATMIIAFLGF MVWAHHMFTV 300
    GMGPVANSIF AVATMTIAVP TGIKIFNWLF TMWGGKITFN TAMLFASSFV 350
    PTFVLGGVTG VMLAMAPVDY LYHDTYFVVA HFHYIIVGGI VLSLFAGLFY 400
    WYPKMFGHML NETLGKLFFW VFYIGFHLTF FVQHLLGLMG MPRRVYTYLG 450
    DQGLDAFNFI STIGTFFMSA GVILLVINVI YSAFKGERVT VADPWDARTL 500
    EWATPTPVPE YNFAQTPQVR SLDPLFYEKI HGDGTMKPAE PVTDIHMPNG 550
    SILPFIMSIG LFFAGFGLIM LNMDNPIINP WIVAIGGLAL TFGCMFVRSI 600
    KEDHGYHIPA EQVKADLAEL KKGGN 625
    Length:625
    Mass (Da):69,871
    Last modified:June 15, 2010 - v3
    Checksum:i6D8058522B7C54A4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti575 – 5751N → K in AAA22365. (PubMed:7678007)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94110 Genomic DNA. Translation: AAA22365.1.
    CP001878 Genomic DNA. Translation: ADC48251.1.
    RefSeqiYP_003425143.1. NC_013791.2.

    Genome annotation databases

    EnsemblBacteriaiADC48251; ADC48251; BpOF4_00910.
    GeneIDi8765154.
    KEGGibpf:BpOF4_00910.
    PATRICi31942830. VBIBacPse80461_0177.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94110 Genomic DNA. Translation: AAA22365.1 .
    CP001878 Genomic DNA. Translation: ADC48251.1 .
    RefSeqi YP_003425143.1. NC_013791.2.

    3D structure databases

    ProteinModelPortali Q04440.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADC48251 ; ADC48251 ; BpOF4_00910 .
    GeneIDi 8765154.
    KEGGi bpf:BpOF4_00910.
    PATRICi 31942830. VBIBacPse80461_0177.

    Phylogenomic databases

    HOGENOMi HOG000085275.
    KOi K02274.

    Enzyme and pathway databases

    UniPathwayi UPA00705 .
    BioCyci BPSE398511:GJI9-2593-MONOMER.

    Family and domain databases

    Gene3Di 1.20.210.10. 1 hit.
    InterProi IPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    IPR014241. Cyt_c_oxidase_su1_bac.
    [Graphical view ]
    PANTHERi PTHR10422. PTHR10422. 1 hit.
    Pfami PF00115. COX1. 1 hit.
    [Graphical view ]
    PRINTSi PR01165. CYCOXIDASEI.
    SUPFAMi SSF81442. SSF81442. 1 hit.
    TIGRFAMsi TIGR02891. CtaD_CoxA. 1 hit.
    PROSITEi PS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the cta operon from alkaliphilic Bacillus firmus OF4 and characterization of the pH-regulated cytochrome caa3 oxidase it encodes."
      Quirk P.G., Hicks D.B., Krulwich T.A.
      J. Biol. Chem. 268:678-685(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13.
    2. "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4."
      Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J., Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B., Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G.
      , Xue Y., Ma Y., Hu F.Z., Krulwich T.A.
      Environ. Microbiol. 13:3289-3309(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: OF4.

    Entry informationi

    Entry nameiCOX1_BACPE
    AccessioniPrimary (citable) accession number: Q04440
    Secondary accession number(s): D3FU49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: June 15, 2010
    Last modified: October 1, 2014
    This is version 102 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3