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Protein

ATP-dependent DNA helicase II subunit 2

Gene

YKU80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Appears to have a role in recruitment of telomerase and CDC13 to the telomere and the subsequent telomere elongation. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.14 Publications

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • damaged DNA binding Source: InterPro
  • DNA binding Source: SGD
  • helicase activity Source: UniProtKB-KW
  • RNA binding Source: SGD
  • telomeric DNA binding Source: SGD

GO - Biological processi

  • chromatin assembly or disassembly Source: SGD
  • chromatin silencing at telomere Source: SGD
  • donor selection Source: SGD
  • double-strand break repair via homologous recombination Source: SGD
  • double-strand break repair via nonhomologous end joining Source: SGD
  • establishment of macromolecular complex localization to telomere Source: SGD
  • protein localization to chromosome Source: SGD
  • telomere maintenance Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32803-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase II subunit 2 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent DNA helicase II subunit Ku80
High affinity DNA-binding factor subunit 2
Yeast Ku80
Gene namesi
Name:YKU80
Synonyms:HDF2
Ordered Locus Names:YMR106C
ORF Names:YM9718.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR106C.
SGDiS000004712. YKU80.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000843391 – 629ATP-dependent DNA helicase II subunit 2Add BLAST629

Proteomic databases

MaxQBiQ04437.
PRIDEiQ04437.

Interactioni

Subunit structurei

Heterodimer of YKU70/HDF1 and YKU80/HDF2. Interacts with SIR4.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35281. 161 interactors.
DIPiDIP-2757N.
IntActiQ04437. 74 interactors.
MINTiMINT-619682.
STRINGi4932.YMR106C.

Structurei

3D structure databases

ProteinModelPortaliQ04437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini254 – 476KuAdd BLAST223

Sequence similaritiesi

Belongs to the ku80 family.Curated

Phylogenomic databases

HOGENOMiHOG000066046.
InParanoidiQ04437.
KOiK10885.
OMAiYRYGADY.
OrthoDBiEOG092C1B2W.

Family and domain databases

Gene3Di2.40.290.10. 1 hit.
3.40.50.410. 1 hit.
InterProiView protein in InterPro
IPR006164. Ku70/Ku80_beta-barrel_dom.
IPR024193. Ku80.
IPR005161. Ku_N.
IPR016194. SPOC-like_C_dom.
IPR002035. VWF_A.
PANTHERiPTHR12604:SF7. PTHR12604:SF7. 1 hit.
PfamiView protein in Pfam
PF02735. Ku. 1 hit.
PF03731. Ku_N. 1 hit.
PIRSFiPIRSF016570. Ku80. 1 hit.
SMARTiView protein in SMART
SM00559. Ku78. 1 hit.
SUPFAMiSSF100939. SSF100939. 2 hits.
SSF53300. SSF53300. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSESTTFIV DVSPSMMKNN NVSKSMAYLE YTLLNKSKKS RKTDWISCYL
60 70 80 90 100
ANCPVSENSQ EIPNVFQIQS FLAPVTTTAT IGFIKRLKQY CDQHSHDSSN
110 120 130 140 150
EGLQSMIQCL LVVSLDIKQQ FQARKILKQI VVFTDNLDDL DITDEEIDLL
160 170 180 190 200
TEELSTRIIL IDCGKDTQEE RKKSNWLKLV EAIPNSRIYN MNELLVEITS
210 220 230 240 250
PATSVVKPVR VFSGELRLGA DILSTQTSNP SGSMQDENCL CIKVEAFPAT
260 270 280 290 300
KAVSGLNRKT AVEVEDSQKK ERYVGVKSII EYEIHNEGNK KNVSEDDQSG
310 320 330 340 350
SSYIPVTISK DSVTKAYRYG ADYVVLPSVL VDQTVYESFP GLDLRGFLNR
360 370 380 390 400
EALPRYFLTS ESSFITADTR LGCQSDLMAF SALVDVMLEN RKIAVARYVS
410 420 430 440 450
KKDSEVNMCA LCPVLIEHSN INSEKKFVKS LTLCRLPFAE DERVTDFPKL
460 470 480 490 500
LDRTTTSGVP LKKETDGHQI DELMEQFVDS MDTDELPEIP LGNYYQPIGE
510 520 530 540 550
VTTDTTLPLP SLNKDQEENK KDPLRIPTVF VYRQQQVLLE WIHQLMINDS
560 570 580 590 600
REFEIPELPD SLKNKISPYT HKKFDSTKLV EVLGIKKVDK LKLDSELKTE
610 620
LEREKIPDLE TLLKRGEQHS RGSPNNSNN
Length:629
Mass (Da):71,241
Last modified:November 1, 1997 - v1
Checksum:i58126164EE375643
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti149L → V in strain: DBVPG6044, SK1 and YPS128. 1
Natural varianti301S → L in strain: DBVPG1853. 1
Natural varianti349N → D in strain: DBVPG6044, SK1 and YPS128. 1
Natural varianti499G → D in strain: DBVPG1853. 1
Natural varianti518E → A in strain: DBVPG6044, SK1 and YPS128. 1
Natural varianti528T → A in strain: DBVPG1853. 1
Natural varianti585I → S in strain: DBVPG6763. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM296333 Genomic DNA. Translation: CAL35984.1.
AM296334 Genomic DNA. Translation: CAL35983.1.
AM296335 Genomic DNA. Translation: CAL35982.1.
AM296336 Genomic DNA. Translation: CAL35981.1.
AM296337 Genomic DNA. Translation: CAL35980.1.
AM296338 Genomic DNA. Translation: CAL35979.1.
AM296339 Genomic DNA. Translation: CAL35978.1.
AM296340 Genomic DNA. Translation: CAL35977.1.
AM296341 Genomic DNA. Translation: CAL35976.1.
AM296342 Genomic DNA. Translation: CAL35975.1.
AM296343 Genomic DNA. Translation: CAL35974.1.
AM296344 Genomic DNA. Translation: CAL35973.1.
AM296345 Genomic DNA. Translation: CAL35972.1.
Z49702 Genomic DNA. Translation: CAA89742.1.
BK006946 Genomic DNA. Translation: DAA10003.1.
PIRiS54567.
RefSeqiNP_013824.1. NM_001182606.1.

Genome annotation databases

EnsemblFungiiYMR106C; YMR106C; YMR106C.
GeneIDi855132.
KEGGisce:YMR106C.

Similar proteinsi

Entry informationi

Entry nameiKU80_YEAST
AccessioniPrimary (citable) accession number: Q04437
Secondary accession number(s): D6VZS9
, Q0P737, Q0P738, Q0P741, Q0P749
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 27, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names