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Protein

Spindle pole body component KRE28

Gene

KRE28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms a kinetochore complex with SPC105 which is required for kinetochore binding by a discrete subset of kMAPs (BIM1, BIK1 and SLK19) and motors (CIN8, KAR3). Involved in kinetochore-microtubule binding and the spindle assembly checkpoint.1 Publication

Enzyme and pathway databases

BioCyciYEAST:G3O-30043-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Spindle pole body component KRE28
Gene namesi
Name:KRE28
Ordered Locus Names:YDR532C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR532C.
SGDiS000002940. KRE28.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: SGD
  • condensed nuclear chromosome kinetochore Source: SGD
  • cytoplasm Source: UniProtKB-KW
  • nuclear membrane Source: UniProtKB-SubCell
  • spindle pole body Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Spindle pole body component KRE28PRO_0000253889Add
BLAST

Proteomic databases

MaxQBiQ04431.

PTM databases

iPTMnetiQ04431.

Interactioni

Subunit structurei

Component of the SPC105 complex composed of at least SPC105 and KRE28. The SPC105 complex interacts with the MIND and NDC80 complexes at the centromere.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SPC105P531483EBI-32446,EBI-23870

Protein-protein interaction databases

BioGridi32581. 57 interactions.
DIPiDIP-1393N.
IntActiQ04431. 9 interactions.
MINTiMINT-408191.

Structurei

3D structure databases

ProteinModelPortaliQ04431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili128 – 16740Sequence analysisAdd
BLAST
Coiled coili229 – 25830Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the KRE28 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiQ04431.
KOiK05749.
OMAiMANESEL.
OrthoDBiEOG7TQV98.

Family and domain databases

InterProiIPR031361. Kre28.
[Graphical view]
PfamiPF17097. Kre28. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTGSASIKD YETVLTDIED SIAVSSEEVL NNQELRLKNT LHEITSSILA
60 70 80 90 100
INEENKFVNP LRNDESLDVE GKEVFVNPKI LSAKIKEFNK LMELLKLTYL
110 120 130 140 150
EQETLDYFFR FTLSSTKPLQ LDSEKDPQFV KLNERVNDLK EEISNVQESK
160 170 180 190 200
IEQIKAEIQE TGHNFAERQD LINELYLEAT GDIENCWDSL NELKNLTNKE
210 220 230 240 250
DKNMMGEKDT ILNSSDSDDF VEETYTNWQK LLFLQKQNQR LTKELKEMHE
260 270 280 290 300
VKNQIIRKGE QSKKEDSGHL MANESELCQS INLLTKFWEK HFLLKGSKTT
310 320 330 340 350
ILNFEIFTQL GKVQFEIKDM QYIIAISLSD LKRPMIKDIT ILQKAGGNIV
360 370 380
TDIEASSKFN NKYRNNTKVQ IFEVMDDIIS ELTNE
Length:385
Mass (Da):44,675
Last modified:November 1, 1996 - v1
Checksum:iA1A44CF0D9E75F50
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti263 – 2631K → R in AAS56906 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33057 Genomic DNA. Translation: AAB64971.1.
AY558580 Genomic DNA. Translation: AAS56906.1.
BK006938 Genomic DNA. Translation: DAA12363.1.
PIRiS69587.
RefSeqiNP_010821.3. NM_001180840.3.

Genome annotation databases

EnsemblFungiiYDR532C; YDR532C; YDR532C.
GeneIDi852145.
KEGGisce:YDR532C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33057 Genomic DNA. Translation: AAB64971.1.
AY558580 Genomic DNA. Translation: AAS56906.1.
BK006938 Genomic DNA. Translation: DAA12363.1.
PIRiS69587.
RefSeqiNP_010821.3. NM_001180840.3.

3D structure databases

ProteinModelPortaliQ04431.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32581. 57 interactions.
DIPiDIP-1393N.
IntActiQ04431. 9 interactions.
MINTiMINT-408191.

PTM databases

iPTMnetiQ04431.

Proteomic databases

MaxQBiQ04431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR532C; YDR532C; YDR532C.
GeneIDi852145.
KEGGisce:YDR532C.

Organism-specific databases

EuPathDBiFungiDB:YDR532C.
SGDiS000002940. KRE28.

Phylogenomic databases

InParanoidiQ04431.
KOiK05749.
OMAiMANESEL.
OrthoDBiEOG7TQV98.

Enzyme and pathway databases

BioCyciYEAST:G3O-30043-MONOMER.

Miscellaneous databases

PROiQ04431.

Family and domain databases

InterProiIPR031361. Kre28.
[Graphical view]
PfamiPF17097. Kre28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Interactions between centromere complexes in Saccharomyces cerevisiae."
    Nekrasov V.S., Smith M.A., Peak-Chew S., Kilmartin J.V.
    Mol. Biol. Cell 14:4931-4946(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH SPC105, SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint."
    Pagliuca C., Draviam V.M., Marco E., Sorger P.K., De Wulf P.
    PLoS ONE 4:E7640-E7640(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPC105, FUNCTION.

Entry informationi

Entry nameiKRE28_YEAST
AccessioniPrimary (citable) accession number: Q04431
Secondary accession number(s): D6VTF3, Q6Q537
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.