4-hydroxybenzoyl-CoA thioesterase

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Q04416 (4HBT_ARTSP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 48. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
4-hydroxybenzoyl-CoA thioesterase
Short name=4-HBA-CoA thioesterase
EC=3.1.2.23

Gene names

Name:	fcbC
Synonyms:	fcbC1

Encoded on

Plasmid pASU1 Ref.1

Organism

Arthrobacter sp.

Taxonomic identifier

1667 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	4-hydroxybenzoyl-CoA + H₂O = 4-hydroxybenzoate + CoA. Ref.5
Pathway	Xenobiotic degradation; 4-chlorobenzoate degradation; 4-hydroxybenzoate from 4-chlorobenzoate: step 3/3.
Subunit structure	Homotetramer. Ref.6
Sequence similarities	Belongs to the thioesterase PaaI family.
Biophysicochemical properties	Kinetic parameters: K_M=243 µM for benzoyl-CoA Ref.5 K_M=1.24 µM for 4-hydroxybenzoyl-CoA Ref.5 K_M=22 µM for 3-hydroxybenzoyl-CoA Ref.5 K_M=29 µM for 2,5-dihydroxybenzoyl-CoA Ref.5 K_M=113 µM for 4-chlorobenzoyl-CoA Ref.5 K_M=140 µM for phenylacetyl-CoA Ref.5 K_M=2200 µM for acetyl-CoA Ref.5 K_M=1100 µM for propionyl-CoA Ref.5 K_M=930 µM for butyryl-CoA Ref.5 K_M=810 µM for crotonyl-CoA Ref.5 pH dependence: Optimum pH is 4.6-9.6. Ref.5
Mass spectrometry	Molecular mass is 16394.40 Da from positions 1 - 151. Determined by ESI. Ref.5

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Molecular_function	4-hydroxybenzoyl-CoA thioesterase activity Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 151

151

4-hydroxybenzoyl-CoA thioesterase

PRO_0000400829

Regions

Region

100 – 102

Substrate binding

Sites

Active site

Ref.6

Experimental info

Mutagenesis

E → A: Drastically reduces catalytic activity. Ref.6

Sequence conflict

V → I in BAB40578. Ref.4

Sequence conflict

A → T in BAB40578. Ref.4

Sequence conflict

114

V → I in BAB40578. Ref.4

Sequence conflict

126

D → N in BAB40578. Ref.4

Secondary structure

151

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q04416 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 95738A4BBD241A7E
Show »

FASTA

151

16,394

        10         20         30         40         50         60 
MHRTSNGSHA TGGNLPDVAS HYPVAYEQTL DGTVGFVIDE MTPERATASV EVTDTLRQRW 

        70         80         90        100        110        120 
GLVHGGAYCA LAEMLATEAT VAVVHEKGMM AVGQSNHTSF FRPVKEGHVR AEAVRIHAGS 

       130        140        150 
TTWFWDVSLR DDAGRLCAVS SMSIAVRPRR D

« Hide

References

[1]	"Cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from Arthrobacter sp. strain SU." Schmitz A., Gartemann K.H., Fiedler J., Grund E., Eichenlaub R. Appl. Environ. Microbiol. 58:4068-4071(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 20407 / SU.
[2]	"Isolation and characterization of IS1409, an insertion element of 4-chlorobenzoate-degrading Arthrobacter sp. strain TM1, and development of a system for transposon mutagenesis." Gartemann K.H., Eichenlaub R. J. Bacteriol. 183:3729-3736(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCIB 12013 / TM1.
[3]	"Cloning of the Arthrobacter sp. FG1 dehalogenase genes and construction of hybrid pathways in Pseudomonas putida strains." Radice F., Orlandi V., Massa V., Battini V., Bertoni G., Reineke W., Barbieri P. Appl. Microbiol. Biotechnol. 75:1111-1118(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FG1.
[4]	"4-chlorobenzoate dehalogenase genes are tandemly duplicated in Arthrobacter sp. FHP1." Miyashita K., Ogawa N., Tsumori Y. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FHP1.
[5]	"Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp. strain SU." Zhuang Z., Gartemann K.H., Eichenlaub R., Dunaway-Mariano D. Appl. Environ. Microbiol. 69:2707-2711(2003) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY. Strain: DSM 20407 / SU.
[6]	"The structure of 4-hydroxybenzoyl-CoA thioesterase from arthrobacter sp. strain SU." Thoden J.B., Zhuang Z., Dunaway-Mariano D., Holden H.M. J. Biol. Chem. 278:43709-43716(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG AND IN COMPLEX WITH PRODUCT, SUBUNIT, ACTIVE SITE, MUTAGENESIS OF GLU-73. Strain: DSM 20407 / SU.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M93187 Genomic DNA. Translation: AAC80224.1.
AF042490 Genomic DNA. Translation: AAF76242.1.
AM231748 Genomic DNA. Translation: CAJ77823.1.
AB041030 Genomic DNA. Translation: BAB40578.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Q4S	X-ray	1.95	A/B	1-151	[»]
1Q4T	X-ray	1.60	A/B	1-151	[»]
1Q4U	X-ray	1.60	A/B	1-151	[»]
3R32	X-ray	1.80	A/B	1-151	[»]
3R34	X-ray	1.80	A/B	1-151	[»]
3R35	X-ray	1.80	A/B	1-151	[»]
3R36	X-ray	1.95	A/B	1-151	[»]
3R37	X-ray	1.80	A/B	1-151	[»]
3R3A	X-ray	1.80	A/B	1-151	[»]
3R3B	X-ray	1.80	A/B	1-151	[»]
3R3C	X-ray	1.80	A/B	1-151	[»]
3R3D	X-ray	1.75	A/B	1-151	[»]
3R3F	X-ray	1.75	A/B	1-151	[»]
3TEA	X-ray	1.80	A/B	1-151	[»]

ProteinModelPortal

Q04416.

SMR

Q04416. Positions 10-151.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

SABIO-RK

Q04416.

UniPathway

UPA01011; UER01022.

Family and domain databases

InterPro

IPR003736. PAAI_dom.
IPR006683. Thioestr_supf.
[Graphical view]

Pfam

PF03061. 4HBT. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00369. unchar_dom_1. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q04416.

Entry information

Entry name

4HBT_ARTSP

Accession

Primary (citable) accession number: Q04416
Secondary accession number(s): Q2A653, Q7BUB8, Q9AJQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents