Q04412 (AGE1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribosylation factor GTPase-activating protein effector protein 1
Short name=ARF GAP effector protein 1

Alternative name(s):
Suppressor of ARF1 thermosensitive mutant protein 1

Gene names

Name:	AGE1
Synonyms:	SAT1
Ordered Locus Names:	YDR524C
ORF Names:	D9719.28

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase-activating protein (GAP) for the ADP ribosylation factors ARF1 and ARF2. May be involved in the endocytic pathway. Ref.3 Ref.6
Subcellular location	Cytoplasm Ref.4.
Miscellaneous	Present with 396 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 1 Arf-GAP domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from genetic interaction Ref.3. Source: SGD intra-Golgi vesicle-mediated transport Inferred from genetic interaction Ref.3. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro
Cellular component	endosome Inferred from direct assay. Source: SGD trans-Golgi network Inferred from direct assay. Source: SGD
Molecular function	ARF GTPase activator activity Inferred from direct assay Ref.6. Source: SGD phospholipid binding Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	ADP-ribosylation factor GTPase-activating protein effector protein 1		PRO_0000074222
Regions
	Domain	170 – 297	128	Arf-GAP
	Zinc finger	186 – 210	25	C4-type

Sequences

	Sequence		Length	Mass (Da)
	Q04412 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3F7682F91EA21EDD	FASTA	482	54,494
	10 20 30 40 50 60 MDFYTTDINK NVVPLFSKGT VARTASKAQY PSWCNNALKL TNILLKSLRC KFQTNRCEDD 70 80 90 100 110 120 RGFEVYCVIL KSIALLMAAK ESLILLQIPP SLPSGFPFRS PQLSFTYLST RLSGSQHKST 130 140 150 160 170 180 HSHHINHQTH PIHSSSSNSN SNNRIPTKTD SSKQHTQHFS FANAGASNRD ELLSIVRKID 190 200 210 220 230 240 KSNLKCCDCG STATVEWVSI NLLCILCIKC SGVHRSLGSH ISKIRSLTLD NFTSLELMHL 250 260 270 280 290 300 LQNNVSNSNV NAIYESNLRN FPVKKITANS DDSERSKFII DKYQFKKFVI DSNQGREASL 310 320 330 340 350 360 KSLIKAIHLD SVFMMQRAIA QSKYSLRELT ASEKEQNDLN HSSIFQYSLK HYEIVDGTPI 370 380 390 400 410 420 FFITEFLLCN GIHIDNLPKI TTNWSPKVLE YWETKLEMYG TFQAVNTSRP RSGPHLNMHS 430 440 450 460 470 480 NVDSASSYNK KHDLRVNIPE RSASASKRWS LSSIPKSSQN LMSPTNLLTM HKSLKLAKKD KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A family of Arf effectors defined as suppressors of the loss of Arf function in the yeast Saccharomyces cerevisiae." Zhang C.-J., Cavenagh M.M., Kahn R.A. J. Biol. Chem. 273:19792-19796(1998) [PubMed: 9677411] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and distinct functions." Zhang C.-J., Bowzard J.B., Anido A., Kahn R.A. Yeast 20:315-330(2003) [PubMed: 12627398] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33057 Genomic DNA. Translation: AAB64964.1. BK006938 Genomic DNA. Translation: DAA12354.1.
PIR	S69580.
RefSeq	NP_010812.1. NM_001180832.1.
3D structure databases
ProteinModelPortal	Q04412.
SMR	Q04412. Positions 169-387.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5273N.
MINT	MINT-553156.
STRING	Q04412.
Proteomic databases
PeptideAtlas	Q04412.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR524C; YDR524C; YDR524C.
GeneID	852136.
KEGG	sce:YDR524C.
NMPDR	fig|4932.3.peg.1586.
Organism-specific databases
CYGD	YDR524c.
SGD	S000002932. AGE1.
Phylogenomic databases
eggNOG	fuNOG10542.
GeneTree	EFGT00050000000186.
OrthoDB	EOG4MD17G.
Gene expression databases
ArrayExpress	Q04412.
Genevestigator	Q04412.
GermOnline	YDR524C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001164. ArfGAP. [Graphical view]
Pfam	PF01412. ArfGap. 1 hit. [Graphical view]
SMART	SM00105. ArfGap. 1 hit. [Graphical view]
SUPFAM	SSF57863. ArfGAP. 1 hit.
PROSITE	PS50115. ARFGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970543.

Entry information

Entry name

AGE1_YEAST

Accession

Primary (citable) accession number: Q04412
Secondary accession number(s): D6VTE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program