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Q04409

- EMI2_YEAST

UniProt

Q04409 - EMI2_YEAST

Protein

Putative glucokinase-2

Gene

EMI2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Putative glucokinase involved in phosphorylation of aldohexoses and glucose uptake By similarity. Involved in sporulation. Required for the full activation of the early meiotic inducer IME1.By similarity1 Publication

    Catalytic activityi

    ATP + D-glucose = ADP + D-glucose 6-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei110 – 1101ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi487 – 4926ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glucokinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-KW
    2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis, Sporulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30035-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative glucokinase-2 (EC:2.7.1.2)
    Alternative name(s):
    Early meiotic induction protein 2
    Glucose kinase 2
    Short name:
    GLK-2
    Gene namesi
    Name:EMI2
    Ordered Locus Names:YDR516C
    ORF Names:D9719.21
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR516c.
    SGDiS000002924. EMI2.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Putative glucokinase-2PRO_0000197604Add
    BLAST

    Proteomic databases

    MaxQBiQ04409.
    PaxDbiQ04409.
    PeptideAtlasiQ04409.

    Expressioni

    Inductioni

    Repressed by glucose through the MIG1 and MIG2 repressors.1 Publication

    Gene expression databases

    GenevestigatoriQ04409.

    Interactioni

    Protein-protein interaction databases

    BioGridi32567. 75 interactions.
    DIPiDIP-4977N.
    IntActiQ04409. 7 interactions.
    MINTiMINT-544411.
    STRINGi4932.YDR516C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04409.
    SMRiQ04409. Positions 27-497.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 231218Hexokinase type-1Add
    BLAST
    Domaini244 – 500257Hexokinase type-2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni159 – 18527Glucose-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the hexokinase family.Curated
    Contains 1 hexokinase type-1 domain.Curated
    Contains 1 hexokinase type-2 domain.Curated

    Phylogenomic databases

    eggNOGiCOG5026.
    GeneTreeiENSGT00390000017159.
    HOGENOMiHOG000162670.
    OMAiANMERAM.
    OrthoDBiEOG79SF68.

    Family and domain databases

    InterProiIPR001312. Hexokinase.
    IPR022673. Hexokinase_C.
    IPR019807. Hexokinase_CS.
    IPR022672. Hexokinase_N.
    [Graphical view]
    PANTHERiPTHR19443. PTHR19443. 1 hit.
    PfamiPF00349. Hexokinase_1. 1 hit.
    PF03727. Hexokinase_2. 1 hit.
    [Graphical view]
    PRINTSiPR00475. HEXOKINASE.
    PROSITEiPS00378. HEXOKINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04409-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFENLHKVN AEALEDAVVE ICSSLQVDAA KLDELTAYFI ECMEKGLNNT    50
    SVGEEKTVDK GLPMIPTYVT SLPNGTERGV LLAADLGGTH FRVCSVTLNG 100
    DGTFDMQQLK SKIPEEYLND KDVTSEELFS YLGRRTRAFV RKHHPELLKS 150
    TGENIKPLKM GFTFSYPVDQ TSLSSGTLIR WTKSFKIEDT VGKDVVRLYQ 200
    EQLDIQGLSM INVVALTNDT VGTFLSHCYT SGSRPSSAGE ISEPVIGCIF 250
    GTGTNGCYME DIENIKKLPD ELRTRLLHEG KTQMCINIEW GSFDNELKHL 300
    SATKYDIDID QKFSPNPGYH LFEKRISGMY LGELLRNILV DLHARGLILG 350
    QYRNYDQLPH RLKTPFQLCS EVLSRIEIDD STNLRETELS FLQSLRLPTT 400
    FEERKAIQNL VRSITRRSAY LAAVPIAAIL IKTNALNKRY HGEVEIGFDG 450
    YVIEYYPGFR SMLRHALALS PIGTEGERKI HLRLAKDGSG VGAALCALVA 500
    Length:500
    Mass (Da):55,921
    Last modified:November 1, 1996 - v1
    Checksum:i7C624ECAB4C57883
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33057 Genomic DNA. Translation: AAB64957.1.
    BK006938 Genomic DNA. Translation: DAA12347.1.
    PIRiS69573.
    RefSeqiNP_010804.3. NM_001180824.3.

    Genome annotation databases

    EnsemblFungiiYDR516C; YDR516C; YDR516C.
    GeneIDi852128.
    KEGGisce:YDR516C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33057 Genomic DNA. Translation: AAB64957.1 .
    BK006938 Genomic DNA. Translation: DAA12347.1 .
    PIRi S69573.
    RefSeqi NP_010804.3. NM_001180824.3.

    3D structure databases

    ProteinModelPortali Q04409.
    SMRi Q04409. Positions 27-497.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32567. 75 interactions.
    DIPi DIP-4977N.
    IntActi Q04409. 7 interactions.
    MINTi MINT-544411.
    STRINGi 4932.YDR516C.

    Proteomic databases

    MaxQBi Q04409.
    PaxDbi Q04409.
    PeptideAtlasi Q04409.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR516C ; YDR516C ; YDR516C .
    GeneIDi 852128.
    KEGGi sce:YDR516C.

    Organism-specific databases

    CYGDi YDR516c.
    SGDi S000002924. EMI2.

    Phylogenomic databases

    eggNOGi COG5026.
    GeneTreei ENSGT00390000017159.
    HOGENOMi HOG000162670.
    OMAi ANMERAM.
    OrthoDBi EOG79SF68.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30035-MONOMER.

    Miscellaneous databases

    NextBioi 970522.

    Gene expression databases

    Genevestigatori Q04409.

    Family and domain databases

    InterProi IPR001312. Hexokinase.
    IPR022673. Hexokinase_C.
    IPR019807. Hexokinase_CS.
    IPR022672. Hexokinase_N.
    [Graphical view ]
    PANTHERi PTHR19443. PTHR19443. 1 hit.
    Pfami PF00349. Hexokinase_1. 1 hit.
    PF03727. Hexokinase_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00475. HEXOKINASE.
    PROSITEi PS00378. HEXOKINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Characterization of three related glucose repressors and genes they regulate in Saccharomyces cerevisiae."
      Lutfiyya L.L., Iyer V.R., DeRisi J., DeVit M.J., Brown P.O., Johnston M.
      Genetics 150:1377-1391(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    4. "Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
      Enyenihi A.H., Saunders W.S.
      Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiEMI2_YEAST
    AccessioniPrimary (citable) accession number: Q04409
    Secondary accession number(s): D6VTD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 10600 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3