SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q04409

- EMI2_YEAST

UniProt

Q04409 - EMI2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Putative glucokinase-2

Gene
EMI2, YDR516C, D9719.21
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Putative glucokinase involved in phosphorylation of aldohexoses and glucose uptake By similarity. Involved in sporulation. Required for the full activation of the early meiotic inducer IME1.1 Publication

Catalytic activityi

ATP + D-glucose = ADP + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi487 – 4926ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glucokinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-KW
  2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis, Sporulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30035-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glucokinase-2 (EC:2.7.1.2)
Alternative name(s):
Early meiotic induction protein 2
Glucose kinase 2
Short name:
GLK-2
Gene namesi
Name:EMI2
Ordered Locus Names:YDR516C
ORF Names:D9719.21
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR516c.
SGDiS000002924. EMI2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Putative glucokinase-2PRO_0000197604Add
BLAST

Proteomic databases

MaxQBiQ04409.
PaxDbiQ04409.
PeptideAtlasiQ04409.

Expressioni

Inductioni

Repressed by glucose through the MIG1 and MIG2 repressors.1 Publication

Gene expression databases

GenevestigatoriQ04409.

Interactioni

Protein-protein interaction databases

BioGridi32567. 75 interactions.
DIPiDIP-4977N.
IntActiQ04409. 7 interactions.
MINTiMINT-544411.
STRINGi4932.YDR516C.

Structurei

3D structure databases

ProteinModelPortaliQ04409.
SMRiQ04409. Positions 27-497.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 231218Hexokinase type-1Add
BLAST
Domaini244 – 500257Hexokinase type-2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni159 – 18527Glucose-binding By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
OMAiANMERAM.
OrthoDBiEOG79SF68.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04409-1 [UniParc]FASTAAdd to Basket

« Hide

MSFENLHKVN AEALEDAVVE ICSSLQVDAA KLDELTAYFI ECMEKGLNNT    50
SVGEEKTVDK GLPMIPTYVT SLPNGTERGV LLAADLGGTH FRVCSVTLNG 100
DGTFDMQQLK SKIPEEYLND KDVTSEELFS YLGRRTRAFV RKHHPELLKS 150
TGENIKPLKM GFTFSYPVDQ TSLSSGTLIR WTKSFKIEDT VGKDVVRLYQ 200
EQLDIQGLSM INVVALTNDT VGTFLSHCYT SGSRPSSAGE ISEPVIGCIF 250
GTGTNGCYME DIENIKKLPD ELRTRLLHEG KTQMCINIEW GSFDNELKHL 300
SATKYDIDID QKFSPNPGYH LFEKRISGMY LGELLRNILV DLHARGLILG 350
QYRNYDQLPH RLKTPFQLCS EVLSRIEIDD STNLRETELS FLQSLRLPTT 400
FEERKAIQNL VRSITRRSAY LAAVPIAAIL IKTNALNKRY HGEVEIGFDG 450
YVIEYYPGFR SMLRHALALS PIGTEGERKI HLRLAKDGSG VGAALCALVA 500
Length:500
Mass (Da):55,921
Last modified:November 1, 1996 - v1
Checksum:i7C624ECAB4C57883
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33057 Genomic DNA. Translation: AAB64957.1.
BK006938 Genomic DNA. Translation: DAA12347.1.
PIRiS69573.
RefSeqiNP_010804.3. NM_001180824.3.

Genome annotation databases

EnsemblFungiiYDR516C; YDR516C; YDR516C.
GeneIDi852128.
KEGGisce:YDR516C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33057 Genomic DNA. Translation: AAB64957.1 .
BK006938 Genomic DNA. Translation: DAA12347.1 .
PIRi S69573.
RefSeqi NP_010804.3. NM_001180824.3.

3D structure databases

ProteinModelPortali Q04409.
SMRi Q04409. Positions 27-497.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32567. 75 interactions.
DIPi DIP-4977N.
IntActi Q04409. 7 interactions.
MINTi MINT-544411.
STRINGi 4932.YDR516C.

Proteomic databases

MaxQBi Q04409.
PaxDbi Q04409.
PeptideAtlasi Q04409.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR516C ; YDR516C ; YDR516C .
GeneIDi 852128.
KEGGi sce:YDR516C.

Organism-specific databases

CYGDi YDR516c.
SGDi S000002924. EMI2.

Phylogenomic databases

eggNOGi COG5026.
GeneTreei ENSGT00390000017159.
HOGENOMi HOG000162670.
OMAi ANMERAM.
OrthoDBi EOG79SF68.

Enzyme and pathway databases

BioCyci YEAST:G3O-30035-MONOMER.

Miscellaneous databases

NextBioi 970522.

Gene expression databases

Genevestigatori Q04409.

Family and domain databases

InterProi IPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view ]
PANTHERi PTHR19443. PTHR19443. 1 hit.
Pfami PF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view ]
PRINTSi PR00475. HEXOKINASE.
PROSITEi PS00378. HEXOKINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Characterization of three related glucose repressors and genes they regulate in Saccharomyces cerevisiae."
    Lutfiyya L.L., Iyer V.R., DeRisi J., DeVit M.J., Brown P.O., Johnston M.
    Genetics 150:1377-1391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  4. "Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
    Enyenihi A.H., Saunders W.S.
    Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEMI2_YEAST
AccessioniPrimary (citable) accession number: Q04409
Secondary accession number(s): D6VTD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10600 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi