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Protein

Putative glucokinase-2

Gene

EMI2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative glucokinase involved in phosphorylation of aldohexoses and glucose uptake (By similarity). Involved in sporulation. Required for the full activation of the early meiotic inducer IME1.By similarity1 Publication

Catalytic activityi

ATP + D-glucose = ADP + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi487 – 4926ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis, Sporulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30035-MONOMER.
ReactomeiREACT_275254. Glycolysis.
REACT_298577. Regulation of gene expression in beta cells.
REACT_302131. Glucose transport.
REACT_318658. Regulation of Glucokinase by Glucokinase Regulatory Protein.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glucokinase-2 (EC:2.7.1.2)
Alternative name(s):
Early meiotic induction protein 2
Glucose kinase 2
Short name:
GLK-2
Gene namesi
Name:EMI2
Ordered Locus Names:YDR516C
ORF Names:D9719.21
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR516c.
EuPathDBiFungiDB:YDR516C.
SGDiS000002924. EMI2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 500499Putative glucokinase-2PRO_0000197604Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei470 – 4701PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ04409.
PaxDbiQ04409.
PeptideAtlasiQ04409.

Expressioni

Inductioni

Repressed by glucose through the MIG1 and MIG2 repressors.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi32567. 76 interactions.
DIPiDIP-4977N.
IntActiQ04409. 7 interactions.
MINTiMINT-544411.

Structurei

3D structure databases

ProteinModelPortaliQ04409.
SMRiQ04409. Positions 27-497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 498487HexokinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni74 – 217144Hexokinase small subdomainPROSITE-ProRule annotationAdd
BLAST
Regioni159 – 18527Glucose-bindingBy similarityAdd
BLAST
Regioni218 – 487270Hexokinase large subdomainPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 1 hexokinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
InParanoidiQ04409.
KOiK00844.
OMAiWIVATEL.
OrthoDBiEOG79SF68.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFENLHKVN AEALEDAVVE ICSSLQVDAA KLDELTAYFI ECMEKGLNNT
60 70 80 90 100
SVGEEKTVDK GLPMIPTYVT SLPNGTERGV LLAADLGGTH FRVCSVTLNG
110 120 130 140 150
DGTFDMQQLK SKIPEEYLND KDVTSEELFS YLGRRTRAFV RKHHPELLKS
160 170 180 190 200
TGENIKPLKM GFTFSYPVDQ TSLSSGTLIR WTKSFKIEDT VGKDVVRLYQ
210 220 230 240 250
EQLDIQGLSM INVVALTNDT VGTFLSHCYT SGSRPSSAGE ISEPVIGCIF
260 270 280 290 300
GTGTNGCYME DIENIKKLPD ELRTRLLHEG KTQMCINIEW GSFDNELKHL
310 320 330 340 350
SATKYDIDID QKFSPNPGYH LFEKRISGMY LGELLRNILV DLHARGLILG
360 370 380 390 400
QYRNYDQLPH RLKTPFQLCS EVLSRIEIDD STNLRETELS FLQSLRLPTT
410 420 430 440 450
FEERKAIQNL VRSITRRSAY LAAVPIAAIL IKTNALNKRY HGEVEIGFDG
460 470 480 490 500
YVIEYYPGFR SMLRHALALS PIGTEGERKI HLRLAKDGSG VGAALCALVA
Length:500
Mass (Da):55,921
Last modified:November 1, 1996 - v1
Checksum:i7C624ECAB4C57883
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33057 Genomic DNA. Translation: AAB64957.1.
BK006938 Genomic DNA. Translation: DAA12347.1.
PIRiS69573.
RefSeqiNP_010804.3. NM_001180824.3.

Genome annotation databases

EnsemblFungiiYDR516C; YDR516C; YDR516C.
GeneIDi852128.
KEGGisce:YDR516C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33057 Genomic DNA. Translation: AAB64957.1.
BK006938 Genomic DNA. Translation: DAA12347.1.
PIRiS69573.
RefSeqiNP_010804.3. NM_001180824.3.

3D structure databases

ProteinModelPortaliQ04409.
SMRiQ04409. Positions 27-497.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32567. 76 interactions.
DIPiDIP-4977N.
IntActiQ04409. 7 interactions.
MINTiMINT-544411.

Proteomic databases

MaxQBiQ04409.
PaxDbiQ04409.
PeptideAtlasiQ04409.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR516C; YDR516C; YDR516C.
GeneIDi852128.
KEGGisce:YDR516C.

Organism-specific databases

CYGDiYDR516c.
EuPathDBiFungiDB:YDR516C.
SGDiS000002924. EMI2.

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
InParanoidiQ04409.
KOiK00844.
OMAiWIVATEL.
OrthoDBiEOG79SF68.

Enzyme and pathway databases

BioCyciYEAST:G3O-30035-MONOMER.
ReactomeiREACT_275254. Glycolysis.
REACT_298577. Regulation of gene expression in beta cells.
REACT_302131. Glucose transport.
REACT_318658. Regulation of Glucokinase by Glucokinase Regulatory Protein.

Miscellaneous databases

NextBioi970522.
PROiQ04409.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Characterization of three related glucose repressors and genes they regulate in Saccharomyces cerevisiae."
    Lutfiyya L.L., Iyer V.R., DeRisi J., DeVit M.J., Brown P.O., Johnston M.
    Genetics 150:1377-1391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  4. "Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
    Enyenihi A.H., Saunders W.S.
    Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEMI2_YEAST
AccessioniPrimary (citable) accession number: Q04409
Secondary accession number(s): D6VTD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: July 22, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.