Reviewed, UniProtKB/Swiss-Prot Q04401 (ACN9_YEAST)

Names and origin

Protein names

Recommended name:
    Acetate non-utilizing protein 9, mitochondrial

Gene names

Name:	ACN9
Ordered Locus Names:	YDR511W
ORF Names:	D9719.16

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the regulation of enzymes involved in acetate assimilation like gluconeogenesis, the glyoxylate cycle, and acetyl-CoA metabolism By similarity.
Subcellular location	Mitochondrion intermembrane space Ref.6 Ref.7.
Miscellaneous	Present with 195 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the ACN9 family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Mitochondrion
Domain	Transit peptide
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbon utilization by utilization of organic compounds Ref.4 Inferred from mutant phenotype. Source: SGD gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW regulation of gluconeogenesis Ref.4 Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial intermembrane space Ref.6 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 12	12	Mitochondrion Potential
	Chain	13 – 133	121	Acetate non-utilizing protein 9, mitochondrial		PRO_0000042652
Experimental info
	Sequence conflict	13	1	A → G in AAB01676. Ref.1
	Sequence conflict	17	1	S → R in AAB01676. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q04401-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 340F408ED251A12D	FASTA	133	15,783
	10 20 30 40 50 60 MNNKLIYRSV RFATHNSQLL LPPLVLYRRI LRQHKLLPGP QREMGDQYVR NEFKLHKDID 70 80 90 100 110 120 NPLHIVGFLA SWQDYLHMIS NGKWKDATLS SETLEKLSPE QTVQLYELMK ETQKLHQDNE 130 IESSKDVKRN NKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Meluh P.B., Koshland D.E. Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YNN 214.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Mutants of Saccharomyces cerevisiae with defects in acetate metabolism: isolation and characterization of Acn- mutants." McCammon M.T. Genetics 144:57-69(1996) [PubMed: 8878673] [Abstract] Cited for: FUNCTION.
[5]	"Yeast mutants of glucose metabolism with defects in the coordinate regulation of carbon assimilation." Dennis R.A., Rhodey M., McCammon M.T. Arch. Biochem. Biophys. 365:279-288(1999) [PubMed: 10328823] [Abstract] Cited for: FUNCTION.
[6]	"Acn9 is a novel protein of gluconeogenesis that is located in the mitochondrial intermembrane space." Dennis R.A., McCammon M.T. Eur. J. Biochem. 261:236-243(1999) [PubMed: 10103055] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U27233 Genomic DNA. Translation: AAB01676.1. U33057 Genomic DNA. Translation: AAB64952.1. AY558175 Genomic DNA. Translation: AAS56501.1.
PIR	S69568.
RefSeq	NP_010799.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2752N.
IntAct	Q04401. 4 interactions.
STRING	Q04401.
Proteomic databases
PeptideAtlas	Q04401.
PRIDE	Q04401.
Genome annotation databases
Ensembl	YDR511W; YDR511W; YDR511W; Saccharomyces cerevisiae. [Genome view]
GeneID	852123.
KEGG	sce:YDR511W.
NMPDR	fig|4932.3.peg.1573.
Organism-specific databases
CYGD	YDR511w.
SGD	S000002919. ACN9.
Phylogenomic databases
HOGENOM	HBG716384.
OMA	NEFKLHK.
OrthoDB	EOG9ZW6TJ.
Gene expression databases
ArrayExpress	Q04401.
Genevestigator	Q04401.
GermOnline	YDR511W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008381. ACN9. [Graphical view]
PANTHER	PTHR13137. ACN9. 1 hit.
Pfam	PF05882. ACN9. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970507.

Entry information

Entry name

ACN9_YEAST

Accession

Primary (citable) accession number: Q04401
Secondary accession number(s): Q02562

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	November 1, 1996
Last modified:	December 15, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)