Adenylate cyclase type 5 - Rattus norvegicus (Rat)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Adenylate cyclase type 5
EC=4.6.1.1

Alternative name(s):
ATP pyrophosphate-lyase 5
Adenylate cyclase type V
Adenylyl cyclase 5

Gene names

Name:

Adcy5

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1262 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is a membrane-bound, calcium-inhibitable adenylyl cyclase.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Enzyme regulation	Inhibition by calcium in the submicromolar concentration range. Phosphorylation by RAF1 results in its activation By similarity.
Subunit structure	Part of a complex containing AKAP5, ADCY6, PDE4C and PKD2 By similarity. Interacts with RAF1 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein. Cell projection › cilium By similarity.
Post-translational modification	Phosphorylated by RAF1 By similarity.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Cell projection Cilium Membrane
Domain	Repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	endosome Inferred from direct assay. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Inferred from direct assay. Source: RGD membrane raft Inferred from direct assay. Source: RGD plasma membrane Inferred from direct assay. Source: RGD primary cilium Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium- and calmodulin-responsive adenylate cyclase activity Inferred from direct assay. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1262

1262

Adenylate cyclase type 5

PRO_0000195697

Regions

Topological domain

1 – 242

242

Cytoplasmic Potential

Transmembrane

243 – 263

21

Helical; Potential

Transmembrane

269 – 289

21

Helical; Potential

Transmembrane

300 – 320

21

Helical; Potential

Transmembrane

326 – 346

21

Helical; Potential

Transmembrane

351 – 371

21

Helical; Potential

Transmembrane

375 – 395

21

Helical; Potential

Topological domain

396 – 763

368

Cytoplasmic Potential

Transmembrane

764 – 784

21

Helical; Potential

Transmembrane

790 – 810

21

Helical; Potential

Transmembrane

837 – 857

21

Helical; Potential

Topological domain

858 – 910

53

Extracellular Potential

Transmembrane

911 – 931

21

Helical; Potential

Transmembrane

936 – 956

21

Helical; Potential

Transmembrane

985 – 1005

21

Helical; Potential

Topological domain

1006 – 1262

257

Cytoplasmic Potential

Domain

470 – 597

128

Guanylate cyclase 1

Domain

1072 – 1211

140

Guanylate cyclase 2

Compositional bias

64 – 67

4

Poly-Gln

Compositional bias

141 – 147

7

Poly-Ala

Sites

Metal binding

475

1

Magnesium 1 By similarity

Metal binding

475

1

Magnesium 2 By similarity

Metal binding

476

1

Magnesium 2; via carbonyl oxygen By similarity

Metal binding

519

1

Magnesium 1 By similarity

Metal binding

519

1

Magnesium 2 By similarity

Amino acid modifications

Modified residue

8

1

Phosphoserine By similarity

Glycosylation

871

1

N-linked (GlcNAc...) Potential

Glycosylation

888

1

N-linked (GlcNAc...) Potential

Glycosylation

973

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q04400 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 8B147E201C5DF601
Show »

FASTA

1,262

139,179

        10         20         30         40         50         60 
MSGSKSVSPP GYAAQTAASP APRGGPEHRA AWGEADSRAN GYPHAPGGST RGSTKRSGGA 

        70         80         90        100        110        120 
VTPQQQQRLA SRWRGGDDDE DPPLSGDDPL VGGFGFSFRS KSAWQERGGD DGGRGSRRQR 

       130        140        150        160        170        180 
RGAAGGGSTR APPAGGSGSS AAAAAAAGGT EVRPRSVEVG LEERRGKGRA AEELEPGTGT 

       190        200        210        220        230        240 
VEDGDGSEDG GSSVASGSGT GTVLSLGACC LALLQIFRSK KFPSDKLERL YQRYFFRLNQ 

       250        260        270        280        290        300 
SSLTMLMAVL VLVCLVMLAF HAARPPLQVV YLAVLAAAVG VILIMAVLCN RAAFHQDHMG 

       310        320        330        340        350        360 
LACYALIAVV LAVQVVGLLL PQPRSASEGI WWTVFFIYTI YTLLPVRMRA AVLSGVLLSA 

       370        380        390        400        410        420 
LHLAISLHTN AQDQFLLKQL VSNVLIFSCT NIVGVCTHYP AEVSQRQAFQ ETRECIQARL 

       430        440        450        460        470        480 
HSQRENQQQE RLLLSVLPRH VAMEMKADIN AKQEDMMFHK IYIQKHDNVS ILFADIEGFT 

       490        500        510        520        530        540 
SLASQCTAQE LVMTLNELFA RFDKLAAENH CLRIKILGDC YYCVSGLPEA RADHAHCCVE 

       550        560        570        580        590        600 
MGMDMIEAIS SVREVTGVNV NMRVGIHSGR VHCGVLGLRK WQFDVWSNDV TLANHMEAGG 

       610        620        630        640        650        660 
KAGRIHITKA TLNYLNGDYE VEPGCGGERN AYLKEHSIET FLILRCTQKR KEEKAMIAKM 

       670        680        690        700        710        720 
NRQRTNSIGH NPPHWGAERP FYNHLGGNQV SKEMKRMGFE DPKDKNAQES ANPEDEVDEF 

       730        740        750        760        770        780 
LGRAIDARSI DRLRSEHVRK FLLTFREPDL EKKYSKQVDD RFGAYVACAS LVFLFICFVQ 

       790        800        810        820        830        840 
ITIVPHSLFM LSFYLSCFLL LALVVFISVI YACVKLFPTP LQTLSRKIVR SKKNSTLVGV 

       850        860        870        880        890        900 
FTITLVFLSA FVNMFMCNSK NLVGCLAEEH NITVNQVNAC HVMESAFNYS LGDEQGFCGS 

       910        920        930        940        950        960 
PQSNCNFPEY FTYSVLLSLL ACSVFLQISC IGKLVLMLAI ELIYVLIVEV PGVTLFDNAD 

       970        980        990       1000       1010       1020 
LLVTANAIDF SNNGTSQCPE HATKVALKVV TPIIISVFVL ALYLHAQQVE STARLDFLWK 

      1030       1040       1050       1060       1070       1080 
LQATEEKEEM EELQAYNRRL LHNILPKDVA AHFLARERRN DELYYQSCEC VAVMFASIAN 

      1090       1100       1110       1120       1130       1140 
FSEFYVELEA NNEGVECLRL LNEIIADFDE IISEDRFRQL EKIKTIGSTY MAASGLNDST 

      1150       1160       1170       1180       1190       1200 
YDKAGKTHIK ALADFAMKLM DQMKYINEHS FNNFQMKIGL NIGPVVAGVI GARKPQYDIW 

      1210       1220       1230       1240       1250       1260 
GNTVNVASRM DSTGVPDRIQ VTTDMYQVLA ANTYQLECRG VVKVKGKGEM MTYFLNGGPP 


LS

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References

[1]	Premont R.T. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Heart, Kidney and Liver.
[2]	"Two members of a widely expressed subfamily of hormone-stimulated adenylyl cyclases." Premont R.T., Chen J., Ma H.-W., Ponnapalli M., Iyengar R. Proc. Natl. Acad. Sci. U.S.A. 89:9809-9813(1992) [PubMed: 1409703] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 164-1262. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96159 mRNA. Translation: AAB39764.1.
IPI	IPI00327925.
RefSeq	NP_072122.1. NM_022600.1.
UniGene	Rn.6278.
3D structure databases
ProteinModelPortal	Q04400.
SMR	Q04400. Positions 455-644, 1063-1238.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04400.
Proteomic databases
PRIDE	Q04400.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	64532.
KEGG	rno:64532.
UCSC	NM_022600. rat.
Organism-specific databases
CTD	111.
RGD	71014. Adcy5.
Phylogenomic databases
eggNOG	roNOG11298.
GeneTree	ENSGT00590000082802.
HOVERGEN	HBG050458.
InParanoid	Q04400.
OrthoDB	EOG4255S0.
Enzyme and pathway databases
BRENDA	4.6.1.1. 5301.
Gene expression databases
ArrayExpress	Q04400.
Genevestigator	Q04400.
GermOnline	ENSRNOG00000002229. Rattus norvegicus.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR018297. A/G_cyclase_CS. IPR009398. Adenylate_cyclase-like. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 2 hits.
KO	K08045.
Pfam	PF06327. DUF1053. 1 hit. PF00211. Guanylate_cyc. 2 hits. [Graphical view]
SMART	SM00044. CYCc. 2 hits. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 2 hits.
PROSITE	PS00452. GUANYLATE_CYCLASE_1. 2 hits. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	613374.

Entry information

Entry name

ADCY5_RAT

Accession

Primary (citable) accession number: Q04400

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents