Q043I0 (FTHS_LACGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	LGAS_1016

Organism

Lactobacillus gasseri (strain ATCC 33323 / DSM 20243) [Complete proteome] [HAMAP]

Taxonomic identifier

324831 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000293041
Regions
	Nucleotide binding	68 – 75	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q043I0 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 56620F6B9F29B750	FASTA	559	60,837
	10 20 30 40 50 60 MNLKSDIEIA QNTKELPIKE IAQKIDLRPE EIELYGNDKA KISWKGINCI KKNQKLGKLI 70 80 90 100 110 120 LVTSISPTPA GEGKSTITIG LGDAIRNQLH KNTLIALREP SMGPVFGLKG GATGGGYAQI 130 140 150 160 170 180 IPMEDINLHF TGDMHALTSA IDTLAALVDN YIYQDNSLEL DPNRILLKRG IDVNDRTLRK 190 200 210 220 230 240 ITIGQGSRFN GIEHEASFAI TVANELMAIL CLATDINDLK KRIGNILVGF SVKDEPVYVK 250 260 270 280 290 300 DLGFEGAIAA LLSTALKPNL VQTLEHTPAI VHGGPFANIA HGANSVIATN TALHLSDYVL 310 320 330 340 350 360 TEAGFGADLG GQKFMDFVSN HLDKRPDAVV VVATVRALKY QAEESTDHLD EENIPALEKG 370 380 390 400 410 420 FANLKRHMEN MAHYGVPVIV LINKFASDTD QELNKLKELV QDDGFECEIV SYHDEGSKGG 430 440 450 460 470 480 IQAAEKVVEL TGKASDFKPV YKPEDSVEDK IAKIAHKIYH AKDIEYSDTA KKQLAEIKKM 490 500 510 520 530 540 GKDELPVIIA KTQYSFTDKK KILGAPEDFI LHVKDLALKN GAGFIVVATG SILDMPGLPK 550 HPAALDIDVD NNGKISGLF

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33323 / DSM 20243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000413 Genomic DNA. Translation: ABJ60392.1.
RefSeq	YP_814830.1. NC_008530.1.
3D structure databases
ProteinModelPortal	Q043I0.
SMR	Q043I0. Positions 6-557.
ModBase	Search...
Protein-protein interaction databases
STRING	Q043I0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4439761.
GenomeReviews	Gene locus LGAS_1016 in contig CP000413_GR.
KEGG	lga:LGAS_1016.
NMPDR	fig|1596.1.peg.235.
PATRIC	22230196. VBILacGas62225_1005.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HBG677721.
OMA	MALTENA.
PhylomeDB	Q043I0.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	LGAS324831:LGAS_1016-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_LACGA

Accession

Primary (citable) accession number: Q043I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program