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Protein

DNA damage checkpoint protein LCD1

Gene

LCD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a complex with the serine/threonine kinase MEC1 which activates checkpoint signaling upon genotoxic stresses. The MEC1-LCD1 complex is recruited by the single-strand-binding protein complex RPA to DNA lesions in order to initiate the DNA repair by homologous recombination, after the MRX-complex and TEL1 are displaced. Required for the recruitment of MEC1 to DNA lesions, the activation of CHK1 and RAD53 kinases and phosphorylation of RAD9 in response to DNA damage. Required for cell growth and meiotic recombination.9 Publications

Miscellaneous

Present with 606 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • damaged DNA binding Source: SGD

GO - Biological processi

  • covalent chromatin modification Source: UniProtKB-KW
  • DNA damage checkpoint Source: SGD
  • DNA repair Source: UniProtKB-KW
  • establishment of protein localization Source: SGD
  • telomere maintenance via telomerase Source: SGD

Keywordsi

Molecular functionChromatin regulator
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-30022-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA damage checkpoint protein LCD1
Alternative name(s):
DNA damage checkpoint protein 2
Lethal, checkpoint-defective, DNA damage-sensitive protein 1
Gene namesi
Name:LCD1
Synonyms:DDC2, PIE1
Ordered Locus Names:YDR499W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR499W.
SGDiS000002907. LCD1.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Localizes to nuclear DNA repair foci with other DNA repair proteins in response to DNA double strand breaks. The recruitment to DNA lesion sites requires the presence of the RPA complex on DNA.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear chromosome Source: SGD

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi177K → A: Impairs dsDNA and ssDNA binding of the MEC1-LCD1 complex. 1 Publication1
Mutagenesisi179R → A: Impairs dsDNA and ssDNA binding of the MEC1-LCD1 complex. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002277151 – 747DNA damage checkpoint protein LCD1Add BLAST747

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1
Modified residuei11PhosphoserineCombined sources1
Modified residuei76PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by MEC1 in a cell cycle dependent manner and in response to DNA damage.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04377.
PRIDEiQ04377.

PTM databases

iPTMnetiQ04377.

Interactioni

Subunit structurei

Forms a complex with MEC1.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32550. 45 interactors.
DIPiDIP-2970N.
IntActiQ04377. 7 interactors.
MINTiMINT-636630.
STRINGi4932.YDR499W.

Structurei

3D structure databases

ProteinModelPortaliQ04377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili62 – 139Sequence analysisAdd BLAST78

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000057105.
InParanoidiQ04377.
KOiK12578.
OMAiFYGLNRI.
OrthoDBiEOG092C0SZ4.

Family and domain databases

InterProiView protein in InterPro
IPR018622. DNA_damage_chkpnt_Lcd1.
PfamiView protein in Pfam
PF09798. LCD1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRETVGEFS SDDDDDILLE LGTRPPRFTQ IPPSSAALQT QIPTTLEVTT
60 70 80 90 100
TTLNNKQSKN DNQLVNQLNK AQGEASMLRD KINFLNIERE KEKNIQAVKV
110 120 130 140 150
NELQVKHLQE LAKLKQELQK LEDEKKFLQM EARGKSKREV ITNVKPPSTT
160 170 180 190 200
LSTNTNTITP DSSSVAIEAK PQSPQSKKRK ISDNLLKKNM VPLNPNRIIP
210 220 230 240 250
DETSLFLESI LLHQIIGADL STIEILNRLK LDYITEFKFK NFVIAKGAPI
260 270 280 290 300
GKSIVSLLLR CKKTLTLDRF IDTLLEDIAV LIKEISVHPN ESKLAVPFLV
310 320 330 340 350
ALMYQIVQFR PSATHNLALK DCFLFICDLI RIYHHVLKVP IHESNMNLHV
360 370 380 390 400
EPQIFQYELI DYLIISYSFD LLEGILRVLQ SHPKQTYMEF FDENILKSFE
410 420 430 440 450
FVYKLALTIS YKPMVNVIFS AVEVVNIITS IILNMDNSSD LKSLISGSWW
460 470 480 490 500
RDCITRLYAL LEKEIKSGDV YNENVDTTTL HMSKYHDFFG LIRNIGDNEL
510 520 530 540 550
GGLISKLIYT DRLQSVPRVI SKEDIGMDSD KFTAPIIGYK MEKWLLKLKD
560 570 580 590 600
EVLNIFENLL MIYGDDATIV NGEMLIHSSK FLSREQALMI ERYVGQDSPN
610 620 630 640 650
LDLRCHLIEH TLTIIYRLWK DHFKQLREEQ IKQVESQLIM SLWRFLVCQT
660 670 680 690 700
ETVTANEREM RDHRHLVDSL HDLTIKDQAS YYEDAFEDLP EYIEEELKMQ
710 720 730 740
LNKRTGRIMQ VKYDEKFQEM ARTILESKSF DLTTLEEADS LYISMGL
Length:747
Mass (Da):86,425
Last modified:November 1, 1996 - v1
Checksum:iE981C1A38F3310BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33057 Genomic DNA. Translation: AAB64941.1.
BK006938 Genomic DNA. Translation: DAA12331.1.
PIRiS69557.
RefSeqiNP_010787.3. NM_001180807.3.

Genome annotation databases

EnsemblFungiiYDR499W; YDR499W; YDR499W.
GeneIDi852110.
KEGGisce:YDR499W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLCD1_YEAST
AccessioniPrimary (citable) accession number: Q04377
Secondary accession number(s): D6VTC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: July 5, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names