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Protein

Type 2A phosphatase-associated protein 42

Gene

TAP42

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in negative regulation of the TOR signaling pathway in response to type of available nitrogen source. Inhibitor of PP2A phosphatase SIT4, which results in inhibition of nuclear export of MSN2, due to lack of dephosphorylation by SIT4. Also required for rapamycin induced activation of expression of many nitrogen discrimination pathway (NDP) genes. In complex with PPH21, required for organization of the actin cytoskeletom during the cell cycle via a Rho GTPase-dependent mechanism.4 Publications

GO - Biological processi

  • negative regulation of signal transduction Source: UniProtKB-KW
  • positive regulation of transcription from RNA polymerase I promoter Source: SGD
  • TOR signaling Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Signal transduction inhibitor

Enzyme and pathway databases

BioCyciYEAST:G3O-32733-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 2A phosphatase-associated protein 42
Gene namesi
Name:TAP42
Ordered Locus Names:YMR028W
ORF Names:YM9711.18, YM9973.01C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR028W.
SGDiS000004630. TAP42.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • extrinsic component of membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Type 2A phosphatase-associated protein 42PRO_0000218623Add
BLAST

Post-translational modificationi

Phosphorylated by TOR kinases. Dephosphorylated by CDC55, TPD3 and SIT4.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04372.
PeptideAtlasiQ04372.

PTM databases

iPTMnetiQ04372.

Interactioni

Subunit structurei

Associates with the PP2a (PPH21 and PPH22) and SIT4 protein phosphatase catalytic subunits. Interacts with PPG1, PPH3 and TIP41.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PPH21P235946EBI-18926,EBI-12745
PPH22P235954EBI-18926,EBI-12752
SIT4P206046EBI-18926,EBI-13707
TOR2P326004EBI-18926,EBI-19385

Protein-protein interaction databases

BioGridi35200. 104 interactions.
DIPiDIP-6380N.
IntActiQ04372. 11 interactions.
MINTiMINT-615134.

Structurei

Secondary structure

1
366
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1815Combined sources
Helixi29 – 5022Combined sources
Helixi62 – 643Combined sources
Helixi67 – 748Combined sources
Helixi75 – 839Combined sources
Helixi93 – 12028Combined sources
Helixi126 – 1338Combined sources
Helixi143 – 1464Combined sources
Helixi156 – 18631Combined sources
Helixi199 – 23133Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V0PX-ray1.80A/B1-234[»]
ProteinModelPortaliQ04372.
SMRiQ04372. Positions 1-234.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04372.

Family & Domainsi

Sequence similaritiesi

Belongs to the IGBP1/TAP42 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000002414.
HOGENOMiHOG000176034.
InParanoidiQ04372.
OMAiYKAREWD.
OrthoDBiEOG7SJDFS.

Family and domain databases

InterProiIPR007304. TAP42-like.
[Graphical view]
PANTHERiPTHR10933. PTHR10933. 1 hit.
PfamiPF04177. TAP42. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04372-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVTEQFND IISLYSTKLE HTSLRQDSPE YQGLLLSTIK KLLNLKTAIF
60 70 80 90 100
DRLALFSTNE TIDDVSTASI KFLAVDYYLG LLISRRQSND SDVAQRQSMK
110 120 130 140 150
LIYLKKSVES FINFLTLLQD YKLLDPLVGE KLGNFKDRYN PQLSELYAQP
160 170 180 190 200
KNNKDLSGAQ LKRKEKIELF QRNKEISTKL HCLELELKNN DEDHDHDELL
210 220 230 240 250
RELYLMRLHH FSLDTINNIE QNLFECEMLS NFLKNSVHEV KSSGTQIRKE
260 270 280 290 300
SNDDDSTGFT DKLENINKPL IDKKGQVLRN FTLVDKRQQL QQKVRGYGQY
310 320 330 340 350
GPTMSVEEFL DKEFEEGRVL QGGEEPEQAP DEENMDWQDR ETYKAREWDE
360
FKESHAKGSG NTMNRG
Length:366
Mass (Da):42,568
Last modified:November 1, 1997 - v1
Checksum:i16F1E236BEC696DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43890 Genomic DNA. Translation: AAC49396.1.
Z49211 Genomic DNA. Translation: CAA89131.1.
Z49213 Genomic DNA. Translation: CAA89143.1.
AY558399 Genomic DNA. Translation: AAS56725.1.
BK006946 Genomic DNA. Translation: DAA09926.1.
PIRiS54030.
RefSeqiNP_013741.1. NM_001182524.1.

Genome annotation databases

EnsemblFungiiYMR028W; YMR028W; YMR028W.
GeneIDi855043.
KEGGisce:YMR028W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43890 Genomic DNA. Translation: AAC49396.1.
Z49211 Genomic DNA. Translation: CAA89131.1.
Z49213 Genomic DNA. Translation: CAA89143.1.
AY558399 Genomic DNA. Translation: AAS56725.1.
BK006946 Genomic DNA. Translation: DAA09926.1.
PIRiS54030.
RefSeqiNP_013741.1. NM_001182524.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V0PX-ray1.80A/B1-234[»]
ProteinModelPortaliQ04372.
SMRiQ04372. Positions 1-234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35200. 104 interactions.
DIPiDIP-6380N.
IntActiQ04372. 11 interactions.
MINTiMINT-615134.

PTM databases

iPTMnetiQ04372.

Proteomic databases

MaxQBiQ04372.
PeptideAtlasiQ04372.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR028W; YMR028W; YMR028W.
GeneIDi855043.
KEGGisce:YMR028W.

Organism-specific databases

EuPathDBiFungiDB:YMR028W.
SGDiS000004630. TAP42.

Phylogenomic databases

GeneTreeiENSGT00390000002414.
HOGENOMiHOG000176034.
InParanoidiQ04372.
OMAiYKAREWD.
OrthoDBiEOG7SJDFS.

Enzyme and pathway databases

BioCyciYEAST:G3O-32733-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ04372.
PROiQ04372.

Family and domain databases

InterProiIPR007304. TAP42-like.
[Graphical view]
PANTHERiPTHR10933. PTHR10933. 1 hit.
PfamiPF04177. TAP42. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases."
    Di Como C.J., Arndt K.T.
    Genes Dev. 10:1904-1916(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeast."
    Jiang Y., Broach J.R.
    EMBO J. 18:2782-2792(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPH21 AND PPH22, PHOSPHORYLATION, DEPHOSPHORYLATION.
  6. "TIP41 interacts with TAP42 and negatively regulates the TOR signaling pathway."
    Jacinto E., Guo B., Arndt K.T., Schmelzle T., Hall M.N.
    Mol. Cell 8:1017-1026(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TIP41.
  7. "Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases."
    Wang H., Wang X., Jiang Y.
    Mol. Biol. Cell 14:4342-4351(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPG1; PPH21; PPH22; PPH3 AND SIT4.
  8. "Multiple roles of Tap42 in mediating rapamycin-induced transcriptional changes in yeast."
    Duevel K., Santhanam A., Garrett S., Schneper L., Broach J.R.
    Mol. Cell 11:1467-1478(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SIT4.
  9. "The Tap42-protein phosphatase type 2A catalytic subunit complex is required for cell cycle-dependent distribution of actin in yeast."
    Wang H., Jiang Y.
    Mol. Cell. Biol. 23:3116-3125(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPH21 AND PPH22.
  10. "PP2A phosphatase activity is required for stress and Tor kinase regulation of yeast stress response factor Msn2p."
    Santhanam A., Hartley A., Duevel K., Broach J.R., Garrett S.
    Eukaryot. Cell 3:1261-1271(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTAP42_YEAST
AccessioniPrimary (citable) accession number: Q04372
Secondary accession number(s): D6VZK2, Q05039
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.