Q04370 (PEX12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisome assembly protein 12

Alternative name(s):
Peroxin-12

Gene names

Name:	PEX12
Ordered Locus Names:	YMR026C
ORF Names:	YM9711.16C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for protein import into peroxisomes By similarity.
Subcellular location	Peroxisome membrane; Single-pass membrane protein Potential.
Miscellaneous	Present with 907 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the pex2/pex10/pex12 family. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane Peroxisome
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into peroxisome matrix Inferred from mutant phenotype PubMed 11370741PubMed 15536088PubMed 9090384. Source: SGD
Cellular_component	integral to peroxisomal membrane Inferred from direct assay PubMed 11370741. Source: SGD
Molecular_function	ubiquitin-protein ligase activity Inferred from direct assay PubMed 19687296. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PEX10	Q05568	12	EBI-2077297,EBI-13194

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Peroxisome assembly protein 12		PRO_0000218617
Regions
	Transmembrane	270 – 286	17	Helical; Potential
	Zinc finger	334 – 373	40	RING-type; degenerate
Experimental info
	Sequence conflict	37	1	I → T in AAT93085. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q04370 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 98E6300C2CBFA16A	FASTA	399	45,992
	10 20 30 40 50 60 MSFYSNLPSA GQSSRGSSTS GRNGVGLEPL YPTIFEIMSS QEIDSLLPAS IRYLLANHLV 70 80 90 100 110 120 ANFPNRYTLR LNKYFFEWFQ AIKGFVEWYH LKTYNSTFID RFYGLQLFSS RDRNLALTQC 130 140 150 160 170 180 LNPKGQSEWP QGLQLNQQQK SVIFLEKIIL PYITAKLDEI LEKISMNNIF SSDETENKWP 190 200 210 220 230 240 KRAFLRIYPF IKKLLALSNL LVKLLFLTKR TGSVSLLQYL FKIEYTTVRP LSSELSGLKE 250 260 270 280 290 300 TKGMDNRLRK TNISSIFALM QGQLSIIPRF LTFMGSQFFP TFIFVLRVYQ WWTTQDMTTK 310 320 330 340 350 360 LQKRVNDLDE DIPRPPFSSH SDKTEDKEGV SEACPVCEKT VQNPCVLETG YVACYPCAIS 370 380 390 YLVNNEGHCP VTNKKLLGCT YNKHTNKWEV VTGIRKLLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49211 Genomic DNA. Translation: CAA89129.1. AY693066 Genomic DNA. Translation: AAT93085.1. BK006946 Genomic DNA. Translation: DAA09924.1.
PIR	S54028.
RefSeq	NP_013739.1. NM_001182522.1.
3D structure databases
ProteinModelPortal	Q04370.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7940N.
IntAct	Q04370. 8 interactions.
MINT	MINT-737262.
STRING	4932.YMR026C.
Proteomic databases
PaxDb	Q04370.
PeptideAtlas	Q04370.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR026C; YMR026C; YMR026C.
GeneID	855041.
KEGG	sce:YMR026C.
Organism-specific databases
CYGD	YMR026c.
SGD	S000004628. PEX12.
Phylogenomic databases
eggNOG	NOG328872.
GeneTree	ENSGT00390000016209.
HOGENOM	HOG000038427.
KO	K13345.
OMA	VANLECA.
OrthoDB	EOG4Z9254.
Gene expression databases
Genevestigator	Q04370.
GermOnline	YMR026C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR017375. PEX12. IPR006845. Pex_N. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
PANTHER	PTHR12888. PTHR12888. 1 hit.
Pfam	PF04757. Pex2_Pex12. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	978262.

Entry information

Entry name

PEX12_YEAST

Accession

Primary (citable) accession number: Q04370
Secondary accession number(s): D6VZK0, Q6B1L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program