Q04364 (YMP8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TPR repeat-containing protein YMR018W

Gene names

Ordered Locus Names:	YMR018W
ORF Names:	YM9711.06

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Unknown, but in contrast to the closely related PEX5, it is not involved in the binding of type-1 peroxisome-targeting sequences (PTS1) nor in peroxisome biogenesis or fatty acid oxidation.
Subunit structure	Interacts according to large scale protein interaction studies with TLG1 Potential. Ref.5
Sequence similarities	Belongs to the peroxisomal targeting signal receptor family. Contains 8 TPR repeats.

Ontologies

Keywords
Domain	Repeat TPR repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into peroxisome matrix Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytosol Inferred from Biological aspect of Ancestor. Source: RefGenome peroxisomal membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	peroxisome matrix targeting signal-1 binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	TPR repeat-containing protein YMR018W		PRO_0000106320
Regions
	Repeat	174 – 207	34	TPR 1
	Repeat	218 – 252	35	TPR 2
	Repeat	254 – 286	33	TPR 3
	Repeat	288 – 320	33	TPR 4
	Repeat	326 – 359	34	TPR 5
	Repeat	360 – 393	34	TPR 6
	Repeat	394 – 427	34	TPR 7
	Repeat	429 – 461	33	TPR 8
Experimental info
	Sequence conflict	9	1	T → P in AAT92739. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q04364 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 8A1302220602B64A	FASTA	514	59,065
	10 20 30 40 50 60 MNEVTCSITG DNPIHKINNG LGLKWNNLGK FSDFQTNDSA ARDARTIDYI FTNCQTGSSI 70 80 90 100 110 120 GKIDFRAALP ADKSQHSGVS EKEFSRLENQ WSKEFSCFPK NKNADVTKPS RNKHEKRSAN 130 140 150 160 170 180 LHNRYFAQYY STAYQQNRIY PCRISYNEHS SVSNGWEFQF KSIENQLLNE LKIENNVEEK 190 200 210 220 230 240 TVGYEYVAEY EETIDFMHML SSVPQTYQFL KSNIYITERD PYKIGCVLMD NGSNLNEVVM 250 260 270 280 290 300 AFEAAISQDP SHINAWLKLG IVNFENESES NGELALRNCL NLDPNNTIAL ENLAIHHINQ 310 320 330 340 350 360 QNESESLKLF HKWILSKFSK VFQPSAGENK DSINKIPKKA HLAHILESLL NMGIEKKDQY 370 380 390 400 410 420 DIYSVLSILY YSDQKIKQSQ KCLEFLLLEK PNNGTIWNRY GAILANTKSY HSAINAYNKC 430 440 450 460 470 480 KQLRPNFTRV RYNLAIAYMN KGDYVKASKM LIEVILLRSK GYEHNKAKMQ NKFMQNLKNA 490 500 510 LIASKNFDSL DLINGSHNTE SLISTLKAIY NKMD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Identification of PEX5p-related novel peroxisome-targeting signal 1 (PTS1)-binding proteins in mammals." Amery L., Sano H., Mannaerts G.P., Snider J., Van Looy J., Fransen M., Van Veldhoven P.P. Biochem. J. 357:635-646(2001) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF INVOLVEMENT IN PTS1 IMPORT.
[5]	"A comprehensive two-hybrid analysis to explore the yeast protein interactome." Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., Sakaki Y. Proc. Natl. Acad. Sci. U.S.A. 98:4569-4574(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TLG1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49211 Genomic DNA. Translation: CAA89120.1. AY692720 Genomic DNA. Translation: AAT92739.1. BK006946 Genomic DNA. Translation: DAA09916.1.
PIR	S54019.
RefSeq	NP_013731.1. NM_001182514.1.
3D structure databases
ProteinModelPortal	Q04364.
SMR	Q04364. Positions 234-461.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4510N.
IntAct	Q04364. 1 interaction.
MINT	MINT-490324.
STRING	4932.YMR018W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR018W; YMR018W; YMR018W.
GeneID	855032.
KEGG	sce:YMR018W.
Organism-specific databases
CYGD	YMR018w.
SGD	S000004620. YMR018W.
Phylogenomic databases
eggNOG	COG0457.
GeneTree	ENSGT00390000013941.
OrthoDB	EOG45MRDN.
Gene expression databases
Genevestigator	Q04364.
GermOnline	YMR018W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.40.10. 2 hits.
InterPro	IPR024111. PTS1R_family. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
PANTHER	PTHR10130. PTHR10130. 1 hit.
SMART	SM00028. TPR. 3 hits. [Graphical view]
PROSITE	PS50005. TPR. 4 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978236.

Entry information

Entry name

YMP8_YEAST

Accession

Primary (citable) accession number: Q04364
Secondary accession number(s): D6VZJ2, Q6B2L0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program