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Protein

Hit family protein 1

Gene

HNT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. Essential for growth on galactose media at elevated temperatures.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei116Tele-AMP-histidine intermediate1 Publication1

GO - Molecular functioni

  • hydrolase activity Source: SGD
  • identical protein binding Source: IntAct
  • nucleotide binding Source: SGD

GO - Biological processi

  • nucleotide metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29524-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hit family protein 1 (EC:3.-.-.-)
Alternative name(s):
Adenosine 5'-monophosphoramidase
Gene namesi
Name:HNT1
Synonyms:HIT1
Ordered Locus Names:YDL125C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL125C.
SGDiS000002283. HNT1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116H → A: Loss of enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001098001 – 158Hit family protein 1Add BLAST158

Proteomic databases

MaxQBiQ04344.
PRIDEiQ04344.

Interactioni

Subunit structurei

Interacts with KIN28.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8416,EBI-8416

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi31938. 74 interactors.
DIPiDIP-2760N.
IntActiQ04344. 4 interactors.
MINTiMINT-484107.

Structurei

3D structure databases

ProteinModelPortaliQ04344.
SMRiQ04344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 129HITPROSITE-ProRule annotationAdd BLAST104

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi114 – 118Histidine triad motif5

Phylogenomic databases

HOGENOMiHOG000061063.
InParanoidiQ04344.
OMAiPETLAGM.
OrthoDBiEOG092C5E8S.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiView protein in InterPro
IPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
PANTHERiPTHR23089. PTHR23089. 1 hit.
PfamiView protein in Pfam
PF01230. HIT. 1 hit.
PRINTSiPR00332. HISTRIAD.
SUPFAMiSSF54197. SSF54197. 1 hit.
PROSITEiView protein in PROSITE
PS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q04344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPLISAPYL TTTKMSAPAT LDAACIFCKI IKSEIPSFKL IETKYSYAFL
60 70 80 90 100
DIQPTAEGHA LIIPKYHGAK LHDIPDEFLT DAMPIAKRLA KAMKLDTYNV
110 120 130 140 150
LQNNGKIAHQ EVDHVHFHLI PKRDEKSGLI VGWPAQETDF DKLGKLHKEL

LAKLEGSD
Length:158
Mass (Da):17,680
Last modified:November 1, 1997 - v2
Checksum:i55BCD6CFCF666954
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56956 Genomic DNA. Translation: CAA40275.1.
Z74173 Genomic DNA. Translation: CAA98693.1.
BK006938 Genomic DNA. Translation: DAA11735.1.
PIRiB39977.
RefSeqiNP_010158.1. NM_001180184.1.

Genome annotation databases

EnsemblFungiiYDL125C; YDL125C; YDL125C.
GeneIDi851432.
KEGGisce:YDL125C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56956 Genomic DNA. Translation: CAA40275.1.
Z74173 Genomic DNA. Translation: CAA98693.1.
BK006938 Genomic DNA. Translation: DAA11735.1.
PIRiB39977.
RefSeqiNP_010158.1. NM_001180184.1.

3D structure databases

ProteinModelPortaliQ04344.
SMRiQ04344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31938. 74 interactors.
DIPiDIP-2760N.
IntActiQ04344. 4 interactors.
MINTiMINT-484107.

Proteomic databases

MaxQBiQ04344.
PRIDEiQ04344.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL125C; YDL125C; YDL125C.
GeneIDi851432.
KEGGisce:YDL125C.

Organism-specific databases

EuPathDBiFungiDB:YDL125C.
SGDiS000002283. HNT1.

Phylogenomic databases

HOGENOMiHOG000061063.
InParanoidiQ04344.
OMAiPETLAGM.
OrthoDBiEOG092C5E8S.

Enzyme and pathway databases

BioCyciYEAST:G3O-29524-MONOMER.

Miscellaneous databases

PROiQ04344.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiView protein in InterPro
IPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
PANTHERiPTHR23089. PTHR23089. 1 hit.
PfamiView protein in Pfam
PF01230. HIT. 1 hit.
PRINTSiPR00332. HISTRIAD.
SUPFAMiSSF54197. SSF54197. 1 hit.
PROSITEiView protein in PROSITE
PS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHNT1_YEAST
AccessioniPrimary (citable) accession number: Q04344
Secondary accession number(s): D6VRM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: March 15, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5410 molecules/cell in log phase SD medium.1 Publication

Caution

It is uncertain whether Met-1 or Met-15 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.