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Protein

Hit family protein 1

Gene

HNT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. Essential for growth on galactose media at elevated temperatures.1 Publication

Miscellaneous

Present with 5410 molecules/cell in log phase SD medium.1 Publication

Caution

It is uncertain whether Met-1 or Met-15 is the initiator.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei116Tele-AMP-histidine intermediate1 Publication1

GO - Molecular functioni

  • hydrolase activity Source: SGD
  • nucleotide binding Source: SGD

GO - Biological processi

  • nucleotide metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29524-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Hit family protein 1 (EC:3.-.-.-)
Alternative name(s):
Adenosine 5'-monophosphoramidase
Gene namesi
Name:HNT1
Synonyms:HIT1
Ordered Locus Names:YDL125C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL125C
SGDiS000002283 HNT1

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116H → A: Loss of enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001098001 – 158Hit family protein 1Add BLAST158

Proteomic databases

MaxQBiQ04344
PaxDbiQ04344
PRIDEiQ04344

Interactioni

Subunit structurei

Interacts with KIN28.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SSA2P105922EBI-8416,EBI-8603

Protein-protein interaction databases

BioGridi3193878 interactors.
DIPiDIP-2760N
IntActiQ04344 9 interactors.
MINTiQ04344
STRINGi4932.YDL125C

Structurei

3D structure databases

ProteinModelPortaliQ04344
SMRiQ04344
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 129HITPROSITE-ProRule annotationAdd BLAST104

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi114 – 118Histidine triad motif5

Phylogenomic databases

HOGENOMiHOG000061063
InParanoidiQ04344
OMAiLASCIFC
OrthoDBiEOG092C5E8S

Family and domain databases

Gene3Di3.30.428.101 hit
InterProiView protein in InterPro
IPR019808 Histidine_triad_CS
IPR001310 Histidine_triad_HIT
IPR011146 HIT-like
IPR036265 HIT-like_sf
PANTHERiPTHR23089 PTHR23089, 1 hit
PfamiView protein in Pfam
PF01230 HIT, 1 hit
PRINTSiPR00332 HISTRIAD
SUPFAMiSSF54197 SSF54197, 1 hit
PROSITEiView protein in PROSITE
PS00892 HIT_1, 1 hit
PS51084 HIT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q04344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPLISAPYL TTTKMSAPAT LDAACIFCKI IKSEIPSFKL IETKYSYAFL
60 70 80 90 100
DIQPTAEGHA LIIPKYHGAK LHDIPDEFLT DAMPIAKRLA KAMKLDTYNV
110 120 130 140 150
LQNNGKIAHQ EVDHVHFHLI PKRDEKSGLI VGWPAQETDF DKLGKLHKEL

LAKLEGSD
Length:158
Mass (Da):17,680
Last modified:November 1, 1997 - v2
Checksum:i55BCD6CFCF666954
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56956 Genomic DNA Translation: CAA40275.1
Z74173 Genomic DNA Translation: CAA98693.1
BK006938 Genomic DNA Translation: DAA11735.1
PIRiB39977
RefSeqiNP_010158.1, NM_001180184.1

Genome annotation databases

EnsemblFungiiYDL125C; YDL125C; YDL125C
GeneIDi851432
KEGGisce:YDL125C

Similar proteinsi

Entry informationi

Entry nameiHNT1_YEAST
AccessioniPrimary (citable) accession number: Q04344
Secondary accession number(s): D6VRM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome