ID   YM54_YEAST              Reviewed;        1088 AA.
AC   Q04336; D6W021;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   27-MAR-2024, entry version 153.
DE   RecName: Full=Uncharacterized protein YMR196W;
GN   OrderedLocusNames=YMR196W; ORFNames=YM9646.09;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA   Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA   Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT   "Analysis of phosphorylation sites on proteins from Saccharomyces
RT   cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299; SER-984 AND SER-1081,
RP   AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1013, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- MISCELLANEOUS: Present with 2220 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z47815; CAA87818.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA10095.1; -; Genomic_DNA.
DR   PIR; S50925; S50925.
DR   RefSeq; NP_013923.1; NM_001182703.1.
DR   AlphaFoldDB; Q04336; -.
DR   BioGRID; 35374; 67.
DR   DIP; DIP-6550N; -.
DR   IntAct; Q04336; 6.
DR   MINT; Q04336; -.
DR   STRING; 4932.YMR196W; -.
DR   iPTMnet; Q04336; -.
DR   MaxQB; Q04336; -.
DR   PaxDb; 4932-YMR196W; -.
DR   PeptideAtlas; Q04336; -.
DR   EnsemblFungi; YMR196W_mRNA; YMR196W; YMR196W.
DR   GeneID; 855236; -.
DR   KEGG; sce:YMR196W; -.
DR   AGR; SGD:S000004809; -.
DR   SGD; S000004809; YMR196W.
DR   VEuPathDB; FungiDB:YMR196W; -.
DR   eggNOG; KOG2161; Eukaryota.
DR   GeneTree; ENSGT00390000017452; -.
DR   HOGENOM; CLU_005386_0_1_1; -.
DR   InParanoid; Q04336; -.
DR   OMA; MKPDGQI; -.
DR   OrthoDB; 5485150at2759; -.
DR   BioCyc; YEAST:G3O-32883-MONOMER; -.
DR   BioGRID-ORCS; 855236; 0 hits in 10 CRISPR screens.
DR   PRO; PR:Q04336; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; Q04336; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0004573; F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity; IBA:GO_Central.
DR   GO; GO:0009311; P:oligosaccharide metabolic process; IEA:InterPro.
DR   GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central.
DR   Gene3D; 1.50.10.10; -; 2.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR004888; Glycoside_hydrolase_63.
DR   PANTHER; PTHR10412:SF10; GLYCO_HYDRO_63 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR10412; MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..1088
FT                   /note="Uncharacterized protein YMR196W"
FT                   /id="PRO_0000203326"
FT   REGION          954..980
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1063..1088
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        954..970
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1063..1079
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         299
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         984
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         1013
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   MOD_RES         1081
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
SQ   SEQUENCE   1088 AA;  126624 MW;  36C2B42BB2749687 CRC64;
     MNKLRDKFVD STVEEERLRE NRNHEKYWYR WGPYLSERSW ATVREDYSLN GDAWSNFPFE
     HANARVFRWG EDGLFGVSDN KQLVCMNVAL WNGKDERLKE RLFGLTGPQG NHGEDVKELY
     FYLDNTPTHS YMKALYKYPF KKAFPYKELV QKNGERGYED KEFEVYDIDG LYRDSETGDN
     PYFDVFFEMA KDDENPSELN FRLTIHNRSK IDSGELYIAP QLFFRNTWAF DGTRTKDKPL
     LERDAEAANL INMTHKKYGN CQMVFQPSPG GFSSGTNEEE EDKEVEDIDP LLLFTDNESN
     LVKLFNEEKN PSEYTKDAFE EYLVQGKTDA VNPENKGTKA CAVYHFKNIP PGEYVTVRYK
     FTNDPKNSIF KAQNLAVVDE DEFDLIFDNR EEEADNFYWR ITPLPISDEL RNLQRQAFSG
     LLWTKQFYNF TYDAWYNGDA NVKPRPPPNR ANGRNKNWKH LYIEDILSMP DKWEYPFFAS
     WDTAFHCIPL AMIDPEFAKR QLDLLTREWY MHPNGQIPAY EWNFNDVNPP VHAWAVYRVF
     KIERNMYNRE DRTFLERVFQ KLLLNFTWWV NRKDTEGKNV FEGGFLGLDN IGVFNRSEPL
     PTGGTLEQAD STGWMAFFSL QMLNIALELA KENPVYEDIA SKFFEHFILI SDSMSFEYAT
     DITGEKCKEV IKQNLWNEAD KFYYDAISWG DHKVQLPIRS LVGLIPLYAS MTLEPSIIKQ
     FRGFKKRVDW FVNNRPEIFD RNIASMSKKG VGERLLLSLV TKERLTAILS RLLDETEFLS
     PYGIRSLSKY HEKHPFEMNV NGVEYMVKYL PGESDSGMFG GNSNWRGPIW FPTSFLIMEA
     LQRFYLYYGS DFKVECPVGS GDYLNLAEVA EELGYRMIHL FVPDENGERA IHYGDHSKFL
     SSDPYFRDYV PFFEYFDGDT GRGLGASHQC GWTALVAKWI SDVGISCVRL PRTPRSSVAT
     TASTESSEQG PKMKRMARRK SAKSLVNYTA TILDLTEEEK RHHRIGGTHS GLTPQSSISS
     DKARHLMEEM NEEEGIHETV VPEDRHNFET KLIGKLKDKV KNMKVTDKAK DEDIDPMDPM
     SPLNKDVS
//