Q04336 (YM54_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein YMR196W

Gene names

Ordered Locus Names:	YMR196W
ORF Names:	YM9646.09

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1088 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Miscellaneous

Present with 2220 molecules/cell in log phase SD medium.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	catalytic activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1088	1088	Uncharacterized protein YMR196W		PRO_0000203326
Amino acid modifications
	Modified residue	299	1	Phosphoserine Ref.7
	Modified residue	957	1	Phosphoserine Ref.7
	Modified residue	963	1	Phosphoserine Ref.7
	Modified residue	964	1	Phosphothreonine Ref.7
	Modified residue	966	1	Phosphoserine Ref.7
	Modified residue	984	1	Phosphoserine Ref.7
	Modified residue	1008	1	Phosphothreonine Ref.6
	Modified residue	1010	1	Phosphoserine Ref.5 Ref.6 Ref.7
	Modified residue	1013	1	Phosphothreonine Ref.3 Ref.6 Ref.7
	Modified residue	1016	1	Phosphoserine Ref.6 Ref.7
	Modified residue	1017	1	Phosphoserine Ref.6
	Modified residue	1019	1	Phosphoserine Ref.6 Ref.7
	Modified residue	1020	1	Phosphoserine Ref.3 Ref.6 Ref.7
	Modified residue	1081	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q04336 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 36C2B42BB2749687	FASTA	1,088	126,624
	10 20 30 40 50 60 MNKLRDKFVD STVEEERLRE NRNHEKYWYR WGPYLSERSW ATVREDYSLN GDAWSNFPFE 70 80 90 100 110 120 HANARVFRWG EDGLFGVSDN KQLVCMNVAL WNGKDERLKE RLFGLTGPQG NHGEDVKELY 130 140 150 160 170 180 FYLDNTPTHS YMKALYKYPF KKAFPYKELV QKNGERGYED KEFEVYDIDG LYRDSETGDN 190 200 210 220 230 240 PYFDVFFEMA KDDENPSELN FRLTIHNRSK IDSGELYIAP QLFFRNTWAF DGTRTKDKPL 250 260 270 280 290 300 LERDAEAANL INMTHKKYGN CQMVFQPSPG GFSSGTNEEE EDKEVEDIDP LLLFTDNESN 310 320 330 340 350 360 LVKLFNEEKN PSEYTKDAFE EYLVQGKTDA VNPENKGTKA CAVYHFKNIP PGEYVTVRYK 370 380 390 400 410 420 FTNDPKNSIF KAQNLAVVDE DEFDLIFDNR EEEADNFYWR ITPLPISDEL RNLQRQAFSG 430 440 450 460 470 480 LLWTKQFYNF TYDAWYNGDA NVKPRPPPNR ANGRNKNWKH LYIEDILSMP DKWEYPFFAS 490 500 510 520 530 540 WDTAFHCIPL AMIDPEFAKR QLDLLTREWY MHPNGQIPAY EWNFNDVNPP VHAWAVYRVF 550 560 570 580 590 600 KIERNMYNRE DRTFLERVFQ KLLLNFTWWV NRKDTEGKNV FEGGFLGLDN IGVFNRSEPL 610 620 630 640 650 660 PTGGTLEQAD STGWMAFFSL QMLNIALELA KENPVYEDIA SKFFEHFILI SDSMSFEYAT 670 680 690 700 710 720 DITGEKCKEV IKQNLWNEAD KFYYDAISWG DHKVQLPIRS LVGLIPLYAS MTLEPSIIKQ 730 740 750 760 770 780 FRGFKKRVDW FVNNRPEIFD RNIASMSKKG VGERLLLSLV TKERLTAILS RLLDETEFLS 790 800 810 820 830 840 PYGIRSLSKY HEKHPFEMNV NGVEYMVKYL PGESDSGMFG GNSNWRGPIW FPTSFLIMEA 850 860 870 880 890 900 LQRFYLYYGS DFKVECPVGS GDYLNLAEVA EELGYRMIHL FVPDENGERA IHYGDHSKFL 910 920 930 940 950 960 SSDPYFRDYV PFFEYFDGDT GRGLGASHQC GWTALVAKWI SDVGISCVRL PRTPRSSVAT 970 980 990 1000 1010 1020 TASTESSEQG PKMKRMARRK SAKSLVNYTA TILDLTEEEK RHHRIGGTHS GLTPQSSISS 1030 1040 1050 1060 1070 1080 DKARHLMEEM NEEEGIHETV VPEDRHNFET KLIGKLKDKV KNMKVTDKAK DEDIDPMDPM SPLNKDVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1013 AND SER-1020, MASS SPECTROMETRY. Strain: 2124.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1010, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1008; SER-1010; THR-1013; SER-1016; SER-1017; SER-1019 AND SER-1020, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299; SER-957; SER-963; THR-964; SER-966; SER-984; SER-1010; THR-1013; SER-1016; SER-1019; SER-1020 AND SER-1081, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z47815 Genomic DNA. Translation: CAA87818.1. BK006946 Genomic DNA. Translation: DAA10095.1.
PIR	S50925.
RefSeq	NP_013923.1. NM_001182703.1.
3D structure databases
ProteinModelPortal	Q04336.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6550N.
IntAct	Q04336. 6 interactions.
MINT	MINT-708375.
STRING	4932.YMR196W.
Proteomic databases
PaxDb	Q04336.
PeptideAtlas	Q04336.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR196W; YMR196W; YMR196W.
GeneID	855236.
KEGG	sce:YMR196W.
Organism-specific databases
CYGD	YMR196w.
SGD	S000004809. YMR196W.
Phylogenomic databases
eggNOG	NOG82826.
GeneTree	ENSGT00390000017946.
HOGENOM	HOG000011547.
OMA	PFFAAWD.
OrthoDB	EOG4NW2TC.
Gene expression databases
Genevestigator	Q04336.
GermOnline	YMR196W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
ProtoNet	Search...
Other
NextBio	978780.

Entry information

Entry name

YM54_YEAST

Accession

Primary (citable) accession number: Q04336
Secondary accession number(s): D6W021

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program