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Q04322

- GYL1_YEAST

UniProt

Q04322 - GYL1_YEAST

Protein

Probable GTPase-activating protein GYL1

Gene

GYL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Probable GTPase-activating protein which stimulates the GTP hydrolysis rate by GYP5 of YPT1 and SEC4. Involved in ER to Golgi trafficking and polarized exocytosis.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. Rab GTPase activator activity Source: InterPro

    GO - Biological processi

    1. ER to Golgi vesicle-mediated transport Source: SGD
    2. exocytosis Source: UniProtKB-KW
    3. protein transport Source: UniProtKB-KW
    4. regulation of exocytosis Source: SGD

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Biological processi

    ER-Golgi transport, Exocytosis, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32879-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable GTPase-activating protein GYL1
    Alternative name(s):
    GYP5-like protein 1
    Gene namesi
    Name:GYL1
    Synonyms:APP2
    Ordered Locus Names:YMR192W
    ORF Names:YM9646.04
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR192w.
    SGDiS000004804. GYL1.

    Subcellular locationi

    Cytoplasm 1 Publication. Bud 1 Publication. Bud neck 1 Publication

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. Golgi-associated vesicle Source: SGD
    4. incipient cellular bud site Source: SGD
    5. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 720720Probable GTPase-activating protein GYL1PRO_0000208062Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei17 – 171Phosphothreonine3 Publications
    Modified residuei37 – 371Phosphoserine3 Publications
    Modified residuei73 – 731Phosphoserine2 Publications
    Modified residuei139 – 1391Phosphoserine2 Publications
    Cross-linki498 – 498Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ04322.
    PaxDbiQ04322.
    PeptideAtlasiQ04322.

    Expressioni

    Gene expression databases

    GenevestigatoriQ04322.

    Interactioni

    Subunit structurei

    Interacts with GYP5 and RVS167. Is part of SEC4-containing complexes.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LSB1P532812EBI-27427,EBI-23329
    LSB3P436036EBI-27427,EBI-22980
    RPL36BO144552EBI-27427,EBI-14624
    RVS167P397438EBI-27427,EBI-14500
    YSC84P327936EBI-27427,EBI-24460

    Protein-protein interaction databases

    BioGridi35370. 36 interactions.
    DIPiDIP-2891N.
    IntActiQ04322. 18 interactions.
    MINTiMINT-436672.
    STRINGi4932.YMR192W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ04322.
    SMRiQ04322. Positions 265-563.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini297 – 477181Rab-GAP TBCPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili572 – 696125Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GYP5 family.Curated
    Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5210.
    GeneTreeiENSGT00750000117238.
    OMAiHEERIEV.
    OrthoDBiEOG72G1GN.

    Family and domain databases

    InterProiIPR000195. Rab-GTPase-TBC_dom.
    [Graphical view]
    PfamiPF00566. RabGAP-TBC. 1 hit.
    [Graphical view]
    SMARTiSM00164. TBC. 1 hit.
    [Graphical view]
    SUPFAMiSSF47923. SSF47923. 2 hits.
    PROSITEiPS50086. TBC_RABGAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q04322-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSNEDIHEE RIEVPRTPHQ TQPEKDSDRI ALRDEISVPE GDEKAYSDEK    50
    VEMATTNASS NFGSNESAKD GESIGAFSNP HEALMQSKLR EESQSKTILP 100
    SDDLSQQLET EESKVEEALK RITSPPLPPR ADCIEESASA LKSSLPPVLA 150
    GNKNDQAPLD RPQLPPRQVV NAETLHLKAP HGNATPSKSP TSAVGNSSSS 200
    TPPTLPPRRI EDPLDLAAQK HFLASTFKRN MLFYKSEDNS IKCDLDKNIL 250
    NLKEDSKKIN NNEIPEEVSS FWLKVIGDYQ NILINDIETL HFQLSRGIPA 300
    AYRLVVWQLV SYAKSKSFDP IYETYLTEMA PFDVQEFENQ LKMMDEVPSE 350
    YVKRISNVLK AYLLFDPECE FSTDIAYIIN MILDVCEEEA NAFGLLVRLM 400
    KVYGLRLLFL PSASEIDILC YKFDRLVEEF YPEIHNHMVE KGVRSSMFLP 450
    GFFTTLFQKK LPTEIQPRIG DMVFLEGIDS IMRILATLLS NSRDHLLKMG 500
    FDDMLELLKS GLLDAYIKQN DGTRGDTLLS NECMDKLLQD SMMKVAITPK 550
    TMKKYSSEYE EIHRLDNEKE VQYKSITEKN LHLQKHVRKL ENDYTSLNRE 600
    HVTIANELVK NRLNIESVLN ENNGYKLQIL DLKKKLDSEK KKQVLGVYVP 650
    NDLKKDLEET MKKNTQVMDE NLKLQDRISE LERLIEEIKT ANKNGTLFEY 700
    SNSKNNPLGA GWSGFKKVFK 720
    Length:720
    Mass (Da):82,131
    Last modified:November 1, 1997 - v1
    Checksum:iE1423DB4F15F7267
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47815 Genomic DNA. Translation: CAA87813.1.
    BK006946 Genomic DNA. Translation: DAA10090.1.
    PIRiS50920.
    RefSeqiNP_013917.1. NM_001182698.1.

    Genome annotation databases

    EnsemblFungiiYMR192W; YMR192W; YMR192W.
    GeneIDi855230.
    KEGGisce:YMR192W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47815 Genomic DNA. Translation: CAA87813.1 .
    BK006946 Genomic DNA. Translation: DAA10090.1 .
    PIRi S50920.
    RefSeqi NP_013917.1. NM_001182698.1.

    3D structure databases

    ProteinModelPortali Q04322.
    SMRi Q04322. Positions 265-563.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35370. 36 interactions.
    DIPi DIP-2891N.
    IntActi Q04322. 18 interactions.
    MINTi MINT-436672.
    STRINGi 4932.YMR192W.

    Proteomic databases

    MaxQBi Q04322.
    PaxDbi Q04322.
    PeptideAtlasi Q04322.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR192W ; YMR192W ; YMR192W .
    GeneIDi 855230.
    KEGGi sce:YMR192W.

    Organism-specific databases

    CYGDi YMR192w.
    SGDi S000004804. GYL1.

    Phylogenomic databases

    eggNOGi COG5210.
    GeneTreei ENSGT00750000117238.
    OMAi HEERIEV.
    OrthoDBi EOG72G1GN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32879-MONOMER.

    Miscellaneous databases

    NextBioi 978768.
    PROi Q04322.

    Gene expression databases

    Genevestigatori Q04322.

    Family and domain databases

    InterProi IPR000195. Rab-GTPase-TBC_dom.
    [Graphical view ]
    Pfami PF00566. RabGAP-TBC. 1 hit.
    [Graphical view ]
    SMARTi SM00164. TBC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47923. SSF47923. 2 hits.
    PROSITEi PS50086. TBC_RABGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    4. "A proteomic strategy for gaining insights into protein sumoylation in yeast."
      Denison C., Rudner A.D., Gerber S.A., Bakalarski C.E., Moazed D., Gygi S.P.
      Mol. Cell. Proteomics 4:246-254(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-498.
      Strain: EJY251-11b.
    5. "Gyp5p and Gyl1p are involved in the control of polarized exocytosis in budding yeast."
      Chesneau L., Dupre S., Burdina A., Roger J., Le Panse S., Jacquet M., Cuif M.-H.
      J. Cell Sci. 117:4757-4767(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH GYP5, IDENTIFICATION IN SEC4-CONTAINING COMPLEXES.
    6. "Interaction of the Saccharomyces cerevisiae cortical actin patch protein Rvs167p with proteins involved in ER to Golgi vesicle trafficking."
      Friesen H., Colwill K., Robertson K., Schub O., Andrews B.
      Genetics 170:555-568(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH GYP5 AND RVS167.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17 AND SER-37, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17; SER-37; SER-73 AND SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGYL1_YEAST
    AccessioniPrimary (citable) accession number: Q04322
    Secondary accession number(s): D6W016
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2010 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3