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Protein

Probable GTPase-activating protein GYL1

Gene

GYL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable GTPase-activating protein which stimulates the GTP hydrolysis rate by GYP5 of YPT1 and SEC4. Involved in ER to Golgi trafficking and polarized exocytosis.2 Publications

GO - Molecular functioni

GO - Biological processi

  • activation of GTPase activity Source: GO_Central
  • ER to Golgi vesicle-mediated transport Source: SGD
  • exocytosis Source: UniProtKB-KW
  • intracellular protein transport Source: GO_Central
  • regulation of exocytosis Source: SGD
  • regulation of vesicle fusion Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

ER-Golgi transport, Exocytosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32879-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable GTPase-activating protein GYL1
Alternative name(s):
GYP5-like protein 1
Gene namesi
Name:GYL1
Synonyms:APP2
Ordered Locus Names:YMR192W
ORF Names:YM9646.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR192W.
SGDiS000004804. GYL1.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • Golgi-associated vesicle Source: SGD
  • incipient cellular bud site Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002080621 – 720Probable GTPase-activating protein GYL1Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei17PhosphothreonineCombined sources1
Modified residuei37PhosphoserineCombined sources1
Modified residuei73PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Cross-linki498Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ04322.
PRIDEiQ04322.

PTM databases

iPTMnetiQ04322.

Interactioni

Subunit structurei

Interacts with GYP5 and RVS167. Is part of SEC4-containing complexes.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LSB1P532812EBI-27427,EBI-23329
LSB3P436036EBI-27427,EBI-22980
RPL36BO144552EBI-27427,EBI-14624
RVS167P397438EBI-27427,EBI-14500
YSC84P327936EBI-27427,EBI-24460

GO - Molecular functioni

Protein-protein interaction databases

BioGridi35370. 36 interactors.
DIPiDIP-2891N.
IntActiQ04322. 18 interactors.
MINTiMINT-436672.

Structurei

3D structure databases

ProteinModelPortaliQ04322.
SMRiQ04322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini297 – 477Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST181

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili572 – 696Sequence analysisAdd BLAST125

Sequence similaritiesi

Belongs to the GYP5 family.Curated
Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00750000117238.
InParanoidiQ04322.
OMAiDIHEERI.
OrthoDBiEOG092C0Q84.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04322-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSNEDIHEE RIEVPRTPHQ TQPEKDSDRI ALRDEISVPE GDEKAYSDEK
60 70 80 90 100
VEMATTNASS NFGSNESAKD GESIGAFSNP HEALMQSKLR EESQSKTILP
110 120 130 140 150
SDDLSQQLET EESKVEEALK RITSPPLPPR ADCIEESASA LKSSLPPVLA
160 170 180 190 200
GNKNDQAPLD RPQLPPRQVV NAETLHLKAP HGNATPSKSP TSAVGNSSSS
210 220 230 240 250
TPPTLPPRRI EDPLDLAAQK HFLASTFKRN MLFYKSEDNS IKCDLDKNIL
260 270 280 290 300
NLKEDSKKIN NNEIPEEVSS FWLKVIGDYQ NILINDIETL HFQLSRGIPA
310 320 330 340 350
AYRLVVWQLV SYAKSKSFDP IYETYLTEMA PFDVQEFENQ LKMMDEVPSE
360 370 380 390 400
YVKRISNVLK AYLLFDPECE FSTDIAYIIN MILDVCEEEA NAFGLLVRLM
410 420 430 440 450
KVYGLRLLFL PSASEIDILC YKFDRLVEEF YPEIHNHMVE KGVRSSMFLP
460 470 480 490 500
GFFTTLFQKK LPTEIQPRIG DMVFLEGIDS IMRILATLLS NSRDHLLKMG
510 520 530 540 550
FDDMLELLKS GLLDAYIKQN DGTRGDTLLS NECMDKLLQD SMMKVAITPK
560 570 580 590 600
TMKKYSSEYE EIHRLDNEKE VQYKSITEKN LHLQKHVRKL ENDYTSLNRE
610 620 630 640 650
HVTIANELVK NRLNIESVLN ENNGYKLQIL DLKKKLDSEK KKQVLGVYVP
660 670 680 690 700
NDLKKDLEET MKKNTQVMDE NLKLQDRISE LERLIEEIKT ANKNGTLFEY
710 720
SNSKNNPLGA GWSGFKKVFK
Length:720
Mass (Da):82,131
Last modified:November 1, 1997 - v1
Checksum:iE1423DB4F15F7267
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47815 Genomic DNA. Translation: CAA87813.1.
BK006946 Genomic DNA. Translation: DAA10090.1.
PIRiS50920.
RefSeqiNP_013917.1. NM_001182698.1.

Genome annotation databases

EnsemblFungiiYMR192W; YMR192W; YMR192W.
GeneIDi855230.
KEGGisce:YMR192W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47815 Genomic DNA. Translation: CAA87813.1.
BK006946 Genomic DNA. Translation: DAA10090.1.
PIRiS50920.
RefSeqiNP_013917.1. NM_001182698.1.

3D structure databases

ProteinModelPortaliQ04322.
SMRiQ04322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35370. 36 interactors.
DIPiDIP-2891N.
IntActiQ04322. 18 interactors.
MINTiMINT-436672.

PTM databases

iPTMnetiQ04322.

Proteomic databases

MaxQBiQ04322.
PRIDEiQ04322.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR192W; YMR192W; YMR192W.
GeneIDi855230.
KEGGisce:YMR192W.

Organism-specific databases

EuPathDBiFungiDB:YMR192W.
SGDiS000004804. GYL1.

Phylogenomic databases

GeneTreeiENSGT00750000117238.
InParanoidiQ04322.
OMAiDIHEERI.
OrthoDBiEOG092C0Q84.

Enzyme and pathway databases

BioCyciYEAST:G3O-32879-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

PROiQ04322.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGYL1_YEAST
AccessioniPrimary (citable) accession number: Q04322
Secondary accession number(s): D6W016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2010 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.