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Q04322

- GYL1_YEAST

UniProt

Q04322 - GYL1_YEAST

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Protein

Probable GTPase-activating protein GYL1

Gene

GYL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable GTPase-activating protein which stimulates the GTP hydrolysis rate by GYP5 of YPT1 and SEC4. Involved in ER to Golgi trafficking and polarized exocytosis.2 Publications

GO - Molecular functioni

  1. Rab GTPase activator activity Source: InterPro

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: SGD
  2. exocytosis Source: UniProtKB-KW
  3. protein transport Source: UniProtKB-KW
  4. regulation of exocytosis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

ER-Golgi transport, Exocytosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32879-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable GTPase-activating protein GYL1
Alternative name(s):
GYP5-like protein 1
Gene namesi
Name:GYL1
Synonyms:APP2
Ordered Locus Names:YMR192W
ORF Names:YM9646.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR192w.
SGDiS000004804. GYL1.

Subcellular locationi

Cytoplasm 1 Publication. Bud 1 Publication. Bud neck 1 Publication

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. Golgi-associated vesicle Source: SGD
  4. incipient cellular bud site Source: SGD
  5. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Probable GTPase-activating protein GYL1PRO_0000208062Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei17 – 171Phosphothreonine2 Publications
Modified residuei37 – 371Phosphoserine2 Publications
Modified residuei73 – 731Phosphoserine1 Publication
Modified residuei139 – 1391Phosphoserine1 Publication
Cross-linki498 – 498Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ04322.
PaxDbiQ04322.
PeptideAtlasiQ04322.

Expressioni

Gene expression databases

GenevestigatoriQ04322.

Interactioni

Subunit structurei

Interacts with GYP5 and RVS167. Is part of SEC4-containing complexes.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LSB1P532812EBI-27427,EBI-23329
LSB3P436036EBI-27427,EBI-22980
RPL36BO144552EBI-27427,EBI-14624
RVS167P397438EBI-27427,EBI-14500
YSC84P327936EBI-27427,EBI-24460

Protein-protein interaction databases

BioGridi35370. 36 interactions.
DIPiDIP-2891N.
IntActiQ04322. 18 interactions.
MINTiMINT-436672.
STRINGi4932.YMR192W.

Structurei

3D structure databases

ProteinModelPortaliQ04322.
SMRiQ04322. Positions 266-553.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini297 – 477181Rab-GAP TBCPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili572 – 696125Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the GYP5 family.Curated
Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5210.
GeneTreeiENSGT00750000117238.
InParanoidiQ04322.
OMAiHEERIEV.
OrthoDBiEOG72G1GN.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04322-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSNEDIHEE RIEVPRTPHQ TQPEKDSDRI ALRDEISVPE GDEKAYSDEK
60 70 80 90 100
VEMATTNASS NFGSNESAKD GESIGAFSNP HEALMQSKLR EESQSKTILP
110 120 130 140 150
SDDLSQQLET EESKVEEALK RITSPPLPPR ADCIEESASA LKSSLPPVLA
160 170 180 190 200
GNKNDQAPLD RPQLPPRQVV NAETLHLKAP HGNATPSKSP TSAVGNSSSS
210 220 230 240 250
TPPTLPPRRI EDPLDLAAQK HFLASTFKRN MLFYKSEDNS IKCDLDKNIL
260 270 280 290 300
NLKEDSKKIN NNEIPEEVSS FWLKVIGDYQ NILINDIETL HFQLSRGIPA
310 320 330 340 350
AYRLVVWQLV SYAKSKSFDP IYETYLTEMA PFDVQEFENQ LKMMDEVPSE
360 370 380 390 400
YVKRISNVLK AYLLFDPECE FSTDIAYIIN MILDVCEEEA NAFGLLVRLM
410 420 430 440 450
KVYGLRLLFL PSASEIDILC YKFDRLVEEF YPEIHNHMVE KGVRSSMFLP
460 470 480 490 500
GFFTTLFQKK LPTEIQPRIG DMVFLEGIDS IMRILATLLS NSRDHLLKMG
510 520 530 540 550
FDDMLELLKS GLLDAYIKQN DGTRGDTLLS NECMDKLLQD SMMKVAITPK
560 570 580 590 600
TMKKYSSEYE EIHRLDNEKE VQYKSITEKN LHLQKHVRKL ENDYTSLNRE
610 620 630 640 650
HVTIANELVK NRLNIESVLN ENNGYKLQIL DLKKKLDSEK KKQVLGVYVP
660 670 680 690 700
NDLKKDLEET MKKNTQVMDE NLKLQDRISE LERLIEEIKT ANKNGTLFEY
710 720
SNSKNNPLGA GWSGFKKVFK
Length:720
Mass (Da):82,131
Last modified:November 1, 1997 - v1
Checksum:iE1423DB4F15F7267
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z47815 Genomic DNA. Translation: CAA87813.1.
BK006946 Genomic DNA. Translation: DAA10090.1.
PIRiS50920.
RefSeqiNP_013917.1. NM_001182698.1.

Genome annotation databases

EnsemblFungiiYMR192W; YMR192W; YMR192W.
GeneIDi855230.
KEGGisce:YMR192W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z47815 Genomic DNA. Translation: CAA87813.1 .
BK006946 Genomic DNA. Translation: DAA10090.1 .
PIRi S50920.
RefSeqi NP_013917.1. NM_001182698.1.

3D structure databases

ProteinModelPortali Q04322.
SMRi Q04322. Positions 266-553.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35370. 36 interactions.
DIPi DIP-2891N.
IntActi Q04322. 18 interactions.
MINTi MINT-436672.
STRINGi 4932.YMR192W.

Proteomic databases

MaxQBi Q04322.
PaxDbi Q04322.
PeptideAtlasi Q04322.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR192W ; YMR192W ; YMR192W .
GeneIDi 855230.
KEGGi sce:YMR192W.

Organism-specific databases

CYGDi YMR192w.
SGDi S000004804. GYL1.

Phylogenomic databases

eggNOGi COG5210.
GeneTreei ENSGT00750000117238.
InParanoidi Q04322.
OMAi HEERIEV.
OrthoDBi EOG72G1GN.

Enzyme and pathway databases

BioCyci YEAST:G3O-32879-MONOMER.

Miscellaneous databases

NextBioi 978768.
PROi Q04322.

Gene expression databases

Genevestigatori Q04322.

Family and domain databases

InterProi IPR000195. Rab-GTPase-TBC_dom.
[Graphical view ]
Pfami PF00566. RabGAP-TBC. 1 hit.
[Graphical view ]
SMARTi SM00164. TBC. 1 hit.
[Graphical view ]
SUPFAMi SSF47923. SSF47923. 2 hits.
PROSITEi PS50086. TBC_RABGAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  4. "A proteomic strategy for gaining insights into protein sumoylation in yeast."
    Denison C., Rudner A.D., Gerber S.A., Bakalarski C.E., Moazed D., Gygi S.P.
    Mol. Cell. Proteomics 4:246-254(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-498.
    Strain: EJY251-11b.
  5. "Gyp5p and Gyl1p are involved in the control of polarized exocytosis in budding yeast."
    Chesneau L., Dupre S., Burdina A., Roger J., Le Panse S., Jacquet M., Cuif M.-H.
    J. Cell Sci. 117:4757-4767(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH GYP5, IDENTIFICATION IN SEC4-CONTAINING COMPLEXES.
  6. "Interaction of the Saccharomyces cerevisiae cortical actin patch protein Rvs167p with proteins involved in ER to Golgi vesicle trafficking."
    Friesen H., Colwill K., Robertson K., Schub O., Andrews B.
    Genetics 170:555-568(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GYP5 AND RVS167.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17 AND SER-37, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17; SER-37; SER-73 AND SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGYL1_YEAST
AccessioniPrimary (citable) accession number: Q04322
Secondary accession number(s): D6W016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2010 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3