Probable GTPase-activating protein GYL1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q04322 (GYL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable GTPase-activating protein GYL1

Alternative name(s):
GYP5-like protein 1

Gene names

Name:	GYL1
Synonyms:	APP2
Ordered Locus Names:	YMR192W
ORF Names:	YM9646.04

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable GTPase-activating protein which stimulates the GTP hydrolysis rate by GYP5 of YPT1 and SEC4. Involved in ER to Golgi trafficking and polarized exocytosis. Ref.7 Ref.8
Subunit structure	Interacts with GYP5 and RVS167. Is part of SEC4-containing complexes. Ref.7 Ref.8
Subcellular location	Cytoplasm. Bud. Bud neck Ref.7.
Miscellaneous	Present with 2010 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GYP5 family. Contains 1 Rab-GAP TBC domain.

Ontologies

Keywords
Biological process	ER-Golgi transport Exocytosis Protein transport Transport
Cellular component	Cytoplasm
Domain	Coiled coil
Molecular function	GTPase activation
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from genetic interaction Ref.8. Source: SGD exocytosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of Rab GTPase activity Inferred from electronic annotation. Source: GOC protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of exocytosis Inferred from genetic interaction Ref.7. Source: SGD
Cellular_component	Golgi-associated vesicle Inferred from direct assay Ref.7. Source: SGD cellular bud neck Inferred from direct assay PubMed 14562095 Ref.7. Source: SGD cellular bud tip Inferred from direct assay Ref.7 PubMed 22842922. Source: SGD incipient cellular bud site Inferred from direct assay Ref.7. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD plasma membrane Inferred from direct assay Ref.7. Source: SGD
Molecular_function	Rab GTPase activator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
LSB3	P43603	3	EBI-27427,EBI-22980
RPL36B	O14455	2	EBI-27427,EBI-14624
RVS167	P39743	5	EBI-27427,EBI-14500
YSC84	P32793	3	EBI-27427,EBI-24460

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 720

720

Probable GTPase-activating protein GYL1

PRO_0000208062

Regions

Domain

297 – 477

181

Rab-GAP TBC

Coiled coil

572 – 696

125

Potential

Amino acid modifications

Modified residue

Phosphothreonine Ref.5 Ref.9

Modified residue

Phosphoserine Ref.5 Ref.6 Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

124

Phosphoserine Ref.9

Modified residue

139

Phosphoserine Ref.9

Cross-link

498

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q04322 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: E1423DB4F15F7267
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FASTA

720

82,131

        10         20         30         40         50         60 
MNSNEDIHEE RIEVPRTPHQ TQPEKDSDRI ALRDEISVPE GDEKAYSDEK VEMATTNASS 

        70         80         90        100        110        120 
NFGSNESAKD GESIGAFSNP HEALMQSKLR EESQSKTILP SDDLSQQLET EESKVEEALK 

       130        140        150        160        170        180 
RITSPPLPPR ADCIEESASA LKSSLPPVLA GNKNDQAPLD RPQLPPRQVV NAETLHLKAP 

       190        200        210        220        230        240 
HGNATPSKSP TSAVGNSSSS TPPTLPPRRI EDPLDLAAQK HFLASTFKRN MLFYKSEDNS 

       250        260        270        280        290        300 
IKCDLDKNIL NLKEDSKKIN NNEIPEEVSS FWLKVIGDYQ NILINDIETL HFQLSRGIPA 

       310        320        330        340        350        360 
AYRLVVWQLV SYAKSKSFDP IYETYLTEMA PFDVQEFENQ LKMMDEVPSE YVKRISNVLK 

       370        380        390        400        410        420 
AYLLFDPECE FSTDIAYIIN MILDVCEEEA NAFGLLVRLM KVYGLRLLFL PSASEIDILC 

       430        440        450        460        470        480 
YKFDRLVEEF YPEIHNHMVE KGVRSSMFLP GFFTTLFQKK LPTEIQPRIG DMVFLEGIDS 

       490        500        510        520        530        540 
IMRILATLLS NSRDHLLKMG FDDMLELLKS GLLDAYIKQN DGTRGDTLLS NECMDKLLQD 

       550        560        570        580        590        600 
SMMKVAITPK TMKKYSSEYE EIHRLDNEKE VQYKSITEKN LHLQKHVRKL ENDYTSLNRE 

       610        620        630        640        650        660 
HVTIANELVK NRLNIESVLN ENNGYKLQIL DLKKKLDSEK KKQVLGVYVP NDLKKDLEET 

       670        680        690        700        710        720 
MKKNTQVMDE NLKLQDRISE LERLIEEIKT ANKNGTLFEY SNSKNNPLGA GWSGFKKVFK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A proteomic strategy for gaining insights into protein sumoylation in yeast." Denison C., Rudner A.D., Gerber S.A., Bakalarski C.E., Moazed D., Gygi S.P. Mol. Cell. Proteomics 4:246-254(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-498, MASS SPECTROMETRY. Strain: EJY251-11b.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17 AND SER-37, MASS SPECTROMETRY. Strain: ADR376.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37, MASS SPECTROMETRY.
[7]	"Gyp5p and Gyl1p are involved in the control of polarized exocytosis in budding yeast." Chesneau L., Dupre S., Burdina A., Roger J., Le Panse S., Jacquet M., Cuif M.-H. J. Cell Sci. 117:4757-4767(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH GYP5, IDENTIFICATION IN SEC4-CONTAINING COMPLEXES.
[8]	"Interaction of the Saccharomyces cerevisiae cortical actin patch protein Rvs167p with proteins involved in ER to Golgi vesicle trafficking." Friesen H., Colwill K., Robertson K., Schub O., Andrews B. Genetics 170:555-568(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH GYP5 AND RVS167.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17; SER-37; SER-47; SER-73; SER-78; SER-124 AND SER-139, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z47815 Genomic DNA. Translation: CAA87813.1. BK006946 Genomic DNA. Translation: DAA10090.1.
PIR	S50920.
RefSeq	NP_013917.1. NM_001182698.1.
3D structure databases
ProteinModelPortal	Q04322.
SMR	Q04322. Positions 266-553.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2891N.
IntAct	Q04322. 16 interactions.
MINT	MINT-436672.
STRING	4932.YMR192W.
Proteomic databases
PaxDb	Q04322.
PeptideAtlas	Q04322.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR192W; YMR192W; YMR192W.
GeneID	855230.
KEGG	sce:YMR192W.
Organism-specific databases
CYGD	YMR192w.
SGD	S000004804. GYL1.
Phylogenomic databases
eggNOG	COG5210.
GeneTree	ENSGT00700000104071.
OMA	LNREHVT.
OrthoDB	EOG43246M.
Gene expression databases
Genevestigator	Q04322.
GermOnline	YMR192W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000195. Rab-GTPase-TBC_dom. [Graphical view]
Pfam	PF00566. RabGAP-TBC. 1 hit. [Graphical view]
SMART	SM00164. TBC. 1 hit. [Graphical view]
SUPFAM	SSF47923. RabGAP_TBC. 2 hits.
PROSITE	PS50086. TBC_RABGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978768.

Entry information

Entry name

GYL1_YEAST

Accession

Primary (citable) accession number: Q04322
Secondary accession number(s): D6W016

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents