Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q04322 (GYL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable GTPase-activating protein GYL1
Alternative name(s):
GYP5-like protein 1
Gene names
Name:GYL1
Synonyms:APP2
Ordered Locus Names:YMR192W
ORF Names:YM9646.04
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length720 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable GTPase-activating protein which stimulates the GTP hydrolysis rate by GYP5 of YPT1 and SEC4. Involved in ER to Golgi trafficking and polarized exocytosis. Ref.5 Ref.6

Subunit structure

Interacts with GYP5 and RVS167. Is part of SEC4-containing complexes. Ref.5 Ref.6

Subcellular location

Cytoplasm. Bud. Bud neck Ref.5.

Miscellaneous

Present with 2010 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GYP5 family.

Contains 1 Rab-GAP TBC domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 720720Probable GTPase-activating protein GYL1
PRO_0000208062

Regions

Domain297 – 477181Rab-GAP TBC
Coiled coil572 – 696125 Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.9
Modified residue171Phosphothreonine Ref.7 Ref.8
Modified residue371Phosphoserine Ref.7 Ref.8
Modified residue731Phosphoserine Ref.8
Modified residue1391Phosphoserine Ref.8
Cross-link498Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q04322 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: E1423DB4F15F7267

FASTA72082,131
        10         20         30         40         50         60 
MNSNEDIHEE RIEVPRTPHQ TQPEKDSDRI ALRDEISVPE GDEKAYSDEK VEMATTNASS 

        70         80         90        100        110        120 
NFGSNESAKD GESIGAFSNP HEALMQSKLR EESQSKTILP SDDLSQQLET EESKVEEALK 

       130        140        150        160        170        180 
RITSPPLPPR ADCIEESASA LKSSLPPVLA GNKNDQAPLD RPQLPPRQVV NAETLHLKAP 

       190        200        210        220        230        240 
HGNATPSKSP TSAVGNSSSS TPPTLPPRRI EDPLDLAAQK HFLASTFKRN MLFYKSEDNS 

       250        260        270        280        290        300 
IKCDLDKNIL NLKEDSKKIN NNEIPEEVSS FWLKVIGDYQ NILINDIETL HFQLSRGIPA 

       310        320        330        340        350        360 
AYRLVVWQLV SYAKSKSFDP IYETYLTEMA PFDVQEFENQ LKMMDEVPSE YVKRISNVLK 

       370        380        390        400        410        420 
AYLLFDPECE FSTDIAYIIN MILDVCEEEA NAFGLLVRLM KVYGLRLLFL PSASEIDILC 

       430        440        450        460        470        480 
YKFDRLVEEF YPEIHNHMVE KGVRSSMFLP GFFTTLFQKK LPTEIQPRIG DMVFLEGIDS 

       490        500        510        520        530        540 
IMRILATLLS NSRDHLLKMG FDDMLELLKS GLLDAYIKQN DGTRGDTLLS NECMDKLLQD 

       550        560        570        580        590        600 
SMMKVAITPK TMKKYSSEYE EIHRLDNEKE VQYKSITEKN LHLQKHVRKL ENDYTSLNRE 

       610        620        630        640        650        660 
HVTIANELVK NRLNIESVLN ENNGYKLQIL DLKKKLDSEK KKQVLGVYVP NDLKKDLEET 

       670        680        690        700        710        720 
MKKNTQVMDE NLKLQDRISE LERLIEEIKT ANKNGTLFEY SNSKNNPLGA GWSGFKKVFK 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]"A proteomic strategy for gaining insights into protein sumoylation in yeast."
Denison C., Rudner A.D., Gerber S.A., Bakalarski C.E., Moazed D., Gygi S.P.
Mol. Cell. Proteomics 4:246-254(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-498.
Strain: EJY251-11b.
[5]"Gyp5p and Gyl1p are involved in the control of polarized exocytosis in budding yeast."
Chesneau L., Dupre S., Burdina A., Roger J., Le Panse S., Jacquet M., Cuif M.-H.
J. Cell Sci. 117:4757-4767(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH GYP5, IDENTIFICATION IN SEC4-CONTAINING COMPLEXES.
[6]"Interaction of the Saccharomyces cerevisiae cortical actin patch protein Rvs167p with proteins involved in ER to Golgi vesicle trafficking."
Friesen H., Colwill K., Robertson K., Schub O., Andrews B.
Genetics 170:555-568(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GYP5 AND RVS167.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17 AND SER-37, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17; SER-37; SER-73 AND SER-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z47815 Genomic DNA. Translation: CAA87813.1.
BK006946 Genomic DNA. Translation: DAA10090.1.
PIRS50920.
RefSeqNP_013917.1. NM_001182698.1.

3D structure databases

ProteinModelPortalQ04322.
SMRQ04322. Positions 271-562.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35370. 36 interactions.
DIPDIP-2891N.
IntActQ04322. 18 interactions.
MINTMINT-436672.
STRING4932.YMR192W.

Proteomic databases

PaxDbQ04322.
PeptideAtlasQ04322.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR192W; YMR192W; YMR192W.
GeneID855230.
KEGGsce:YMR192W.

Organism-specific databases

CYGDYMR192w.
SGDS000004804. GYL1.

Phylogenomic databases

eggNOGCOG5210.
GeneTreeENSGT00750000117238.
OMAHEERIEV.
OrthoDBEOG72G1GN.

Enzyme and pathway databases

BioCycYEAST:G3O-32879-MONOMER.

Gene expression databases

GenevestigatorQ04322.

Family and domain databases

InterProIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMSSF47923. SSF47923. 2 hits.
PROSITEPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978768.
PROQ04322.

Entry information

Entry nameGYL1_YEAST
AccessionPrimary (citable) accession number: Q04322
Secondary accession number(s): D6W016
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families