Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar basic amino acid transporter 1

Gene

VBA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transporter required for vacuolar uptake of at least histidine and lysine.1 Publication

GO - Molecular functioni

  • basic amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • basic amino acid transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32788-MONOMER.

Protein family/group databases

TCDBi2.A.1.48.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar basic amino acid transporter 1
Gene namesi
Name:VBA1
Ordered Locus Names:YMR088C
ORF Names:YM9582.13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR088C.
SGDiS000004694. VBA1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30VacuolarSequence analysisAdd BLAST30
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Topological domaini52 – 100CytoplasmicSequence analysisAdd BLAST49
Transmembranei101 – 121HelicalSequence analysisAdd BLAST21
Topological domaini122 – 131VacuolarSequence analysis10
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Topological domaini153 – 166CytoplasmicSequence analysisAdd BLAST14
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 190VacuolarSequence analysis3
Transmembranei191 – 211HelicalSequence analysisAdd BLAST21
Topological domaini212 – 232CytoplasmicSequence analysisAdd BLAST21
Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
Topological domaini254 – 255VacuolarSequence analysis2
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Topological domaini277 – 292CytoplasmicSequence analysisAdd BLAST16
Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
Topological domaini314 – 331VacuolarSequence analysisAdd BLAST18
Transmembranei332 – 352HelicalSequence analysisAdd BLAST21
Topological domaini353 – 365CytoplasmicSequence analysisAdd BLAST13
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Topological domaini387 – 419VacuolarSequence analysisAdd BLAST33
Transmembranei420 – 440HelicalSequence analysisAdd BLAST21
Topological domaini441 – 448CytoplasmicSequence analysis8
Transmembranei449 – 469HelicalSequence analysisAdd BLAST21
Topological domaini470 – 528VacuolarSequence analysisAdd BLAST59
Transmembranei529 – 549HelicalSequence analysisAdd BLAST21
Topological domaini550 – 562CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001734281 – 562Vacuolar basic amino acid transporter 1Add BLAST562

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi123N-linked (GlcNAc...)Sequence analysis1
Glycosylationi324N-linked (GlcNAc...)Sequence analysis1
Glycosylationi504N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ04301.
PRIDEiQ04301.

Interactioni

Protein-protein interaction databases

BioGridi35263. 9 interactors.
DIPiDIP-888N.

Structurei

3D structure databases

ProteinModelPortaliQ04301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000141762.
InParanoidiQ04301.
OMAiFYLWRSI.
OrthoDBiEOG092C2834.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTLDETSNL LPPPEEAEAP PLEQKFHEYN LALPKFPILF SLWLGSFLSS
60 70 80 90 100
LDSTIVANIM NRVAEEFSES SKKQWIATSF LLTNTAFQPL YGKLSDITGR
110 120 130 140 150
KSALLTAQFF FGLGCLLTCF ARNVTEFSIA RAICGIGAGG LNAISSIAVS
160 170 180 190 200
DICTARERGV YQGYANIVFG FGQLLGAPLG GVFIETIGWR ALFGIQVPVI
210 220 230 240 250
MLCSVLAIKN INIKLFHVPP MKERYTLKNL SRIDIFGSLS LVATISGVLF
260 270 280 290 300
LCSSQLNKLY LALFTIGSFI VFILVERYYA TEKILPFELL TRSFCLSSAV
310 320 330 340 350
TVISSFVVFG EIFRSPIYLQ LLQNISVTKT GLFLIFPSIS VAVGSLVTGW
360 370 380 390 400
VLRNTKINLA HCAYQIIFGG MIMQLLGLGL GYFLLSHLNP DYTIYDMLES
410 420 430 440 450
ITFRSNSIWW KLIYVFASVL VSFGYACLLV ATLVSIVFTV EKSQQGTMTG
460 470 480 490 500
VFYLWRSIGN VLGASLTLVS YENSLSSMLW NYMFKTKRDD EYHFTKKQYY
510 520 530 540 550
SLINDSSYLR GPNFPTDIFV RILDVYKKAF LISYIPNIAL AAVGIVLSLY
560
LVKHTYKRSS SS
Length:562
Mass (Da):62,640
Last modified:November 1, 1997 - v1
Checksum:i08C6675482A5F3F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49259 Genomic DNA. Translation: CAA89235.1.
BK006946 Genomic DNA. Translation: DAA09985.1.
PIRiS54464.
RefSeqiNP_013806.1. NM_001182588.1.

Genome annotation databases

EnsemblFungiiYMR088C; YMR088C; YMR088C.
GeneIDi855113.
KEGGisce:YMR088C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49259 Genomic DNA. Translation: CAA89235.1.
BK006946 Genomic DNA. Translation: DAA09985.1.
PIRiS54464.
RefSeqiNP_013806.1. NM_001182588.1.

3D structure databases

ProteinModelPortaliQ04301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35263. 9 interactors.
DIPiDIP-888N.

Protein family/group databases

TCDBi2.A.1.48.1. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBiQ04301.
PRIDEiQ04301.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR088C; YMR088C; YMR088C.
GeneIDi855113.
KEGGisce:YMR088C.

Organism-specific databases

EuPathDBiFungiDB:YMR088C.
SGDiS000004694. VBA1.

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000141762.
InParanoidiQ04301.
OMAiFYLWRSI.
OrthoDBiEOG092C2834.

Enzyme and pathway databases

BioCyciYEAST:G3O-32788-MONOMER.

Miscellaneous databases

PROiQ04301.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVBA1_YEAST
AccessioniPrimary (citable) accession number: Q04301
Secondary accession number(s): D6VZR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.