Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar basic amino acid transporter 1

Gene

VBA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transporter required for vacuolar uptake of at least histidine and lysine.1 Publication

GO - Molecular functioni

  • basic amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • basic amino acid transmembrane transport Source: GOC
  • basic amino acid transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32788-MONOMER.

Protein family/group databases

TCDBi2.A.1.48.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar basic amino acid transporter 1
Gene namesi
Name:VBA1
Ordered Locus Names:YMR088C
ORF Names:YM9582.13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR088C.
SGDiS000004694. VBA1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030VacuolarSequence analysisAdd
BLAST
Transmembranei31 – 5121HelicalSequence analysisAdd
BLAST
Topological domaini52 – 10049CytoplasmicSequence analysisAdd
BLAST
Transmembranei101 – 12121HelicalSequence analysisAdd
BLAST
Topological domaini122 – 13110VacuolarSequence analysis
Transmembranei132 – 15221HelicalSequence analysisAdd
BLAST
Topological domaini153 – 16614CytoplasmicSequence analysisAdd
BLAST
Transmembranei167 – 18721HelicalSequence analysisAdd
BLAST
Topological domaini188 – 1903VacuolarSequence analysis
Transmembranei191 – 21121HelicalSequence analysisAdd
BLAST
Topological domaini212 – 23221CytoplasmicSequence analysisAdd
BLAST
Transmembranei233 – 25321HelicalSequence analysisAdd
BLAST
Topological domaini254 – 2552VacuolarSequence analysis
Transmembranei256 – 27621HelicalSequence analysisAdd
BLAST
Topological domaini277 – 29216CytoplasmicSequence analysisAdd
BLAST
Transmembranei293 – 31321HelicalSequence analysisAdd
BLAST
Topological domaini314 – 33118VacuolarSequence analysisAdd
BLAST
Transmembranei332 – 35221HelicalSequence analysisAdd
BLAST
Topological domaini353 – 36513CytoplasmicSequence analysisAdd
BLAST
Transmembranei366 – 38621HelicalSequence analysisAdd
BLAST
Topological domaini387 – 41933VacuolarSequence analysisAdd
BLAST
Transmembranei420 – 44021HelicalSequence analysisAdd
BLAST
Topological domaini441 – 4488CytoplasmicSequence analysis
Transmembranei449 – 46921HelicalSequence analysisAdd
BLAST
Topological domaini470 – 52859VacuolarSequence analysisAdd
BLAST
Transmembranei529 – 54921HelicalSequence analysisAdd
BLAST
Topological domaini550 – 56213CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 562562Vacuolar basic amino acid transporter 1PRO_0000173428Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence analysis
Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence analysis
Glycosylationi504 – 5041N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ04301.
PeptideAtlasiQ04301.

Interactioni

Protein-protein interaction databases

BioGridi35263. 9 interactions.
DIPiDIP-888N.

Structurei

3D structure databases

ProteinModelPortaliQ04301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000141762.
InParanoidiQ04301.
OMAiFYLWRSI.
OrthoDBiEOG799317.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q04301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTLDETSNL LPPPEEAEAP PLEQKFHEYN LALPKFPILF SLWLGSFLSS
60 70 80 90 100
LDSTIVANIM NRVAEEFSES SKKQWIATSF LLTNTAFQPL YGKLSDITGR
110 120 130 140 150
KSALLTAQFF FGLGCLLTCF ARNVTEFSIA RAICGIGAGG LNAISSIAVS
160 170 180 190 200
DICTARERGV YQGYANIVFG FGQLLGAPLG GVFIETIGWR ALFGIQVPVI
210 220 230 240 250
MLCSVLAIKN INIKLFHVPP MKERYTLKNL SRIDIFGSLS LVATISGVLF
260 270 280 290 300
LCSSQLNKLY LALFTIGSFI VFILVERYYA TEKILPFELL TRSFCLSSAV
310 320 330 340 350
TVISSFVVFG EIFRSPIYLQ LLQNISVTKT GLFLIFPSIS VAVGSLVTGW
360 370 380 390 400
VLRNTKINLA HCAYQIIFGG MIMQLLGLGL GYFLLSHLNP DYTIYDMLES
410 420 430 440 450
ITFRSNSIWW KLIYVFASVL VSFGYACLLV ATLVSIVFTV EKSQQGTMTG
460 470 480 490 500
VFYLWRSIGN VLGASLTLVS YENSLSSMLW NYMFKTKRDD EYHFTKKQYY
510 520 530 540 550
SLINDSSYLR GPNFPTDIFV RILDVYKKAF LISYIPNIAL AAVGIVLSLY
560
LVKHTYKRSS SS
Length:562
Mass (Da):62,640
Last modified:November 1, 1997 - v1
Checksum:i08C6675482A5F3F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49259 Genomic DNA. Translation: CAA89235.1.
BK006946 Genomic DNA. Translation: DAA09985.1.
PIRiS54464.
RefSeqiNP_013806.1. NM_001182588.1.

Genome annotation databases

EnsemblFungiiYMR088C; YMR088C; YMR088C.
GeneIDi855113.
KEGGisce:YMR088C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49259 Genomic DNA. Translation: CAA89235.1.
BK006946 Genomic DNA. Translation: DAA09985.1.
PIRiS54464.
RefSeqiNP_013806.1. NM_001182588.1.

3D structure databases

ProteinModelPortaliQ04301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35263. 9 interactions.
DIPiDIP-888N.

Protein family/group databases

TCDBi2.A.1.48.1. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBiQ04301.
PeptideAtlasiQ04301.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR088C; YMR088C; YMR088C.
GeneIDi855113.
KEGGisce:YMR088C.

Organism-specific databases

EuPathDBiFungiDB:YMR088C.
SGDiS000004694. VBA1.

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000141762.
InParanoidiQ04301.
OMAiFYLWRSI.
OrthoDBiEOG799317.

Enzyme and pathway databases

BioCyciYEAST:G3O-32788-MONOMER.

Miscellaneous databases

NextBioi978452.
PROiQ04301.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A family of basic amino acid transporters of the vacuolar membrane from Saccharomyces cerevisiae."
    Shimazu M., Sekito T., Akiyama K., Ohsumi Y., Kakinuma Y.
    J. Biol. Chem. 280:4851-4857(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiVBA1_YEAST
AccessioniPrimary (citable) accession number: Q04301
Secondary accession number(s): D6VZR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.